Abstract: A biodisc comprises a CD-type optical disc with small feature oligonucleotide probes disposed on its surfaces. The biodisc's probes are either custom-fabricated in-situ with a master-duplicate tandem arrangement of discs that allows one disc and a reading/tracking head to control the probe locations being synthesized pass-by-pass on the duplicate. Or the biodisc is mass-produced in a manufacturing process that includes a spin-on-and-peel (SOAP) method to create a nickel stamper for standardized biodisc oligonucleotide probes. Such biodisc is fabricated with four masks only, and these are shifted between depositions to synthesize particular individual 4-mer+ target nucleotide probes.

Abstract: A biodisc comprises a CD-type optical disc with small feature oligonucleotide probes disposed on its surfaces. The biodisc's probes are either custom-fabricated in-situ with a master-duplicate tandem arrangement of discs that allows one disc and a reading/tracking head to control the probe locations being synthesized pass-by-pass on the duplicate. Or the biodisc is mass-produced in a manufacturing process that includes a spin-on-and-peel (SOAP) method with nickel master for standardized biodisc oligonucleotide probes. In one embodiment of the invention, such biodisc is fabricated with four masks only, and these are shifted between depositions to synthesize particular individual 4-mer+ nucleotide probes.