Abstract: A reconstruction system is presented for reconstructing morphology of biological cells. The system includes data input and output utilities, memory, and a data processor, and is configured for data communication with an image data provider to receive raw image data comprising a sequence of image frames acquired from the cell during a movement of the cell and being indicative of optical path delay (OPD) of light propagation through the biological cell. The data processor is configured and operable to process the raw image data and determine 3D dynamic morphological data of the cell. The data processor includes: a modeling utility; a position recovery module; and an analyzer module.