Abstract: According to the process of the invention, sets of restriction fragments from the DNA of an individual are prepared, these restriction fragments being separated by size. Probes are prepared by enzymatic hydrolysis of DNA from a genome bank of the species to which this individual belongs, separation of the fragments obtained as a function of their size, labeling these probes and placing them in contact, under hybridization conditions, with the aforementioned sets of restriction fragments. Finally, selection is made of the probes (capable of hybridizing with said set of restriction fragments) which do not give hybridization profiles identical to those obtained with known probes recognizing variable number tandem repeat regions. Applications for the probes thus obtained include, particularly, processes for identifying an individual, consanguinity testing, and investigating the origin of a seed.

Abstract: According to the process of the invention, sets of restriction fragments from the DNA of an individual are prepared, these restriction fragments being separated by size. Probes are prepared by enzymatic hydrolysis of DNA from a genome bank of the species to which this individual belongs, separation of the fragments obtained as a function of their size, labeling these probes and placing them in contact, under hybridization conditions, with the aforementioned sets of restriction fragments. Finally, selection is made of the probes (capable of hybridizing with said set of restriction fragments) which do not give hybridization profiles identical to those obtained with known probes recognizing variable number tandem repeat regions. Applications for the probes thus obtained include, particularly, processes for identifying an individual, consanguinity testing, and investigating the origin of a seed.

Abstract: A process for detection of a new polymorphic locus in a DNA sequence is characterized by the following stages. Polynucleotides are prepared by forming an unspecified oligonucleotide including 5 to 10 bases which are repeated 5 to 100 times. A genomic bank is explored by hybridization under low stringency conditions using the prepared polynucleotides which are labelled. The clones that react with the polynucleotides are analyzed by the Southern method. The restriction fragments detected by the polynucleotides are purified and labelled. The genomic DNA of non-consanguineous individuals is analyzed by the Southern method under highly stringent hybridization conditions, with the obtained restriction fragments. The restriction fragments corresponding to new polymorphic loci are selected.