Patents by Inventor Giuliana Franzosi
Giuliana Franzosi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11326192Abstract: The present invention relates to a variant of oleaginous yeast of the species Trichosporon oleaginosus characterized by mutations affecting cell wall synthesis that change the morphology thereof with respect to the wild-type strain of the same species. In particular, thanks to said mutations, cell aggregates are formed which, with respect to the wild-type strain of the same species, lower the viscosity of the culture broth, are more easily separable therefrom and thus make their recovery easier. Said variant of oleaginous yeast of the species Trichosporon oleaginosus is also characterized by yields of oleaginous cellular biomass and intracellular accumulation of lipids that are similar or even higher than those of the wild-type strain. Further, the present invention relates to a process for the production of lipids through said variant of oleaginous yeast of the species Trichosporon oleaginosus.Type: GrantFiled: July 17, 2018Date of Patent: May 10, 2022Assignee: Versalis S.p.A.Inventors: Valentina Rodighiero, Giuliana Franzosi, Concetta Compagno, Claudia Capusoni
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Patent number: 10889841Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: GrantFiled: January 30, 2019Date of Patent: January 12, 2021Assignee: ENI S.p.A.Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
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Publication number: 20200149076Abstract: The present invention relates to a variant of oleaginous yeast of the species Trichosporon oleaginosus characterized by mutations affecting cell wall synthesis that change the morphology thereof with respect to the wild-type strain of the same species. In particular, thanks to said mutations, cell aggregates are formed which, with respect to the wild-type strain of the same species, lower the viscosity of the culture broth, are more easily separable therefrom and thus make their recovery easier. Said variant of oleaginous yeast of the species Trichosporon oleaginosus is also characterized by yields of oleaginous cellular biomass and intracellular accumulation of lipids that are similar or even higher than those of the wild-type strain. Further, the present invention relates to a process for the production of lipids through said variant of oleaginous yeast of the species Trichosporon oleaginosus.Type: ApplicationFiled: July 17, 2018Publication date: May 14, 2020Applicant: Versalis S.p.A.Inventors: Valentina RODIGHIERO, Giuliana FRANZOSI, Concetta COMPAGNO, Claidia CAPUSONI
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Publication number: 20190161778Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: ApplicationFiled: January 30, 2019Publication date: May 30, 2019Applicant: ENI S.p.A.Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
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Patent number: 10301656Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: GrantFiled: December 29, 2015Date of Patent: May 28, 2019Assignee: ENI S.p.A.Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
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Publication number: 20170369911Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra- cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.Type: ApplicationFiled: December 29, 2015Publication date: December 28, 2017Applicant: ENI S.p.A.Inventors: Giuliana FRANZOSI, Daniela CUCCHETTI, Daniele BIANCHI, Silvia GALAFASSI, Concetta COMPAGNO
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Patent number: 9650275Abstract: Integrated process for the production of bio-oil from sludge coming from a wastewater purification plant comprising the following steps: (a) sending wastewater to said purification plant obtaining sludge; (b) subjecting the sludge obtained in said step (a) to liquefaction, obtaining a mixture Including an oily phase consisting of bio-oil, a solid phase and an aqueous phase; (c) sending the aqueous phase obtained in said step (b) to said purification plant. The bio-oil (or “bio-crude”) thus obtained can be advantageously used in the production of bio-fuels which can be used as such, or in a mixture with other automotive fuels. Otherwise, said bio-oil (or “bio-crude”) can be used as such (bio-fuel), or in a mixture with fossil fuels (combustible oil, coal, etc.), for the generation of electric energy or heat.Type: GrantFiled: February 24, 2012Date of Patent: May 16, 2017Assignee: ENI S.P.A.Inventors: Aldo Bosetti, Daniele Bianchi, Giuliana Franzosi
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Patent number: 9234221Abstract: Process for the production of lipids from biomass comprising polysaccharides. The biomass is subjected to acid hydrolysis to obtain a solid phase and an aqueous phase. The solid phase is subjected to acid or to enzymatic hydrolysis obtaining a further solid phase and a further aqueous phase. The aqueous phases obtained from both hydrolysis steps are subjected to fermentation with an oleaginous yeast. The obtained fermentation broth comprises oleaginous cellular biomass which is subjected to microfiltration for the isolation of lipids. Said lipids can be used in the production of biodiesel or green diesel.Type: GrantFiled: October 14, 2011Date of Patent: January 12, 2016Assignee: ENI S.P.A.Inventors: Giuliana Franzosi, Francesca Pizza, Daniela Cucchetti, Daniele Bianchi, Concetta Maria Compagno, Silvia Galafassi
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Patent number: 9006502Abstract: A process for the production of bio-oil from solid urban waste, comprising the following steps: a) subjecting said solid urban waste to liquefaction, obtaining a mixture including an oily phase consisting of bio-oil, a solid phase and an aqueous phase; b) subjecting the aqueous phase obtained in the liquefaction step a) to fermentation, obtaining a fermented biomass; c) feeding the fermented biomass obtained in the fermentation step b) to the liquefaction step a). The bio-oil (or bio-crude) thus obtained can be advantageously used in the production of biofuels which can be used as such or mixed with other motor vehicle fuels. Alternatively, this bio-oil (or bio-crude) can be used as such (biocombustible) or mixed with fossil combustibles (combustible oil, coal, etc.) for the generation of electric energy or heat.Type: GrantFiled: September 1, 2010Date of Patent: April 14, 2015Assignee: ENI S.p.A.Inventors: Aldo Bosetti, Daniele Bianchi, Giuliana Franzosi, Marco Ricci
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Patent number: 8999164Abstract: Process for the purification of an aqueous stream coming from the Fischer-Tropsch reaction which comprises: —feeding said aqueous stream containing the organic by-products of the reaction to a distillation column, obtaining two output streams: —an aqueous stream (i) exiting from the column head, enriched with alcohols having from 1 to 20 carbon atoms, preferably from 1 to 9 carbon atoms, and other optional volatile compounds; an aqueous stream (ii) exiting from the column bottom, enriched with organic acids having from 1 to 10 carbon atoms, preferably from 2 to 6 carbon atoms; —subjecting said aqueous stream (ii) to an anaerobic biological treatment obtaining a purified aqueous stream (iii); wherein said anaerobic biological treatment is carried out with a hydraulic retention time lower than 15 hours, preferably ranging from 4 hours to 10 hours, more preferably ranging from 5 hours to 8 hours.Type: GrantFiled: February 18, 2010Date of Patent: April 7, 2015Assignee: ENI S.p.A.Inventors: Giuliana Franzosi, Pietro Cesti, Roberto Paglino, Lino Carnelli, Roberta Miglio
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Patent number: 8969605Abstract: A process for producing bio-oil from municipal solid waste, the process including: a) liquifying municipal solid waste, to obtain a mixture containing an oily phase containing bio-oil, a solid phase, and a first aqueous phase; b) treating the first aqueous phase from a) with an adsorbing material, to obtain a second aqueous phase; c) fermenting the second aqueous phase from b), to obtain a biomass; d) subjecting the biomass obtained in c) to the liquification a). The bio-oil obtained is advantageously used in the production of biofuels for motor vehicles or for the generation of electric energy or heat.Type: GrantFiled: March 30, 2011Date of Patent: March 3, 2015Assignee: ENI S.p.A.Inventors: Aldo Bosetti, Giuliana Franzosi
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Publication number: 20140020284Abstract: Integrated process for the production of bio-oil from sludge coming from a wastewater purification plant comprising the following steps: (a) sending wastewater to said purification plant obtaining sludge; (b) subjecting the sludge obtained in said step (a) to liquefaction, obtaining a mixture Including an oily phase consisting of bio-oil, a solid phase and an aqueous phase; (c) sending the aqueous phase obtained in said step (b) to said purification plant. The bio-oil (or “bio-crude”) thus obtained can be advantageously used in the production of bio-fuels which can be used as such, or in a mixture with other automotive fuels. Otherwise, said bio-oil (or “bio-crude”) can be used as such (bio-fuel), or in a mixture with fossil fuels (combustible oil, coal, etc.), for the generation of electric energy or heat.Type: ApplicationFiled: February 24, 2012Publication date: January 23, 2014Applicant: Eni S.P.A.Inventors: Aldo Bosetti, Daniele Bianchi, Giuliana Franzosi
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Publication number: 20130289289Abstract: Process for the production of lipids from biomass comprising polysaccharides. The biomass is subjected to acid hydrolysis to obtain a solid phase and an aqueous phase. The solid phase is subjected to acid or to enzymatic hydrolysis obtaining a further solid phase and a further aqueous phase. The aqueous phases obtained from both hydrolysis steps are subjected to fermentation with an oleaginous yeast. The obtained fermentation broth comprises oleaginous cellular biomass which is subjected to microfiltration for the isolation of lipids. Said lipids can be used in the production of biodiesel or green diesel.Type: ApplicationFiled: October 14, 2011Publication date: October 31, 2013Applicant: Eni S.P.A.Inventors: Giuliana Franzosi, Francesca Pizza, Daniela Cucchetti, Daniele Bianchi, Concetta Maria Compagno, Silvia Galafassi
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Publication number: 20130090487Abstract: A process for producing bio-oil from municipal solid waste, the process including: a) liquifying municipal solid waste, to obtain a mixture containing an oily phase containing bio-oil, a solid phase, and a first aqueous phase; b) treating the first aqueous phase from a) with an adsorbing material, to obtain a second aqueous phase; c) fermenting the second aqueous phase from b), to obtain a biomass; d) subjecting the biomass obtained in c) to the liquification a). The bio-oil obtained is advantageously used in the production of biofuels for motor vehicles or for the generation of electric energy or heat.Type: ApplicationFiled: March 30, 2011Publication date: April 11, 2013Applicant: ENI S.p.A.Inventors: Aldo Bosetti, Giuliana Franzosi
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Publication number: 20120172642Abstract: A process for the production of bio-oil from solid urban waste, comprising the following steps: a) subjecting said solid urban waste to liquefaction, obtaining a mixture including an oily phase consisting of bio-oil, a solid phase and an aqueous phase; b) subjecting the aqueous phase obtained in the liquefaction step a) to fermentation, obtaining a fermented biomass; c) feeding the fermented biomass obtained in the fermentation step b) to the liquefaction step a). The bio-oil (or bio-crude) thus obtained can be advantageously used in the production of biofuels which can be used as such or mixed with other motor vehicle fuels. Alternatively, this bio-oil (or bio-crude) can be used as such (biocombustible) or mixed with fossil combustibles (combustible oil, coal, etc.) for the generation of electric energy or heat.Type: ApplicationFiled: September 1, 2010Publication date: July 5, 2012Applicant: ENI S.p.A.Inventors: Aldo Bosetti, Daniele Bianchi, Giuliana Franzosi, Marco Ricci
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Publication number: 20120055868Abstract: Process for the purification of an aqueous stream coming from the Fischer-Tropsch reaction which comprises: —feeding said aqueous stream containing the organic by-products of the reaction to a distillation column, obtaining two output streams: —an aqueous stream (i) exiting from the column head, enriched with alcohols having from 1 to 20 carbon atoms, preferably from 1 to 9 carbon atoms, and other optional volatile compounds; an aqueous stream (ii) exiting from the column bottom, enriched with organic acids having from 1 to 10 carbon atoms, preferably from 2 to 6 carbon atoms; —subjecting said aqueous stream (ii) to an anaerobic biological treatment obtaining a purified aqueous stream (iii); wherein said anaerobic biological treatment is carried out with a hydraulic retention time lower than 15 hours, preferably ranging from 4 hours to 10 hours, more preferably ranging from 5 hours to 8 hours.Type: ApplicationFiled: February 18, 2010Publication date: March 8, 2012Applicant: ENI S.p.A.Inventors: Giuliana Franzosi, Pietro Cesti, Roberto Paglino, Lino Carnelli, Roberta Miglio
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Patent number: 5801048Abstract: The present invention relates to a process for producing large amounts of 7.beta.-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture.Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium.The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7.beta.-(4-carboxybutanamido)-cephalosporanic acid.Type: GrantFiled: September 11, 1996Date of Patent: September 1, 1998Assignee: Ministero Dell `Universita` E Della Ricerca Scientifica E TecnologicaInventors: Wilhelmus Van Der Goes, Antonella Bernardi, Aldo Bosetti, Giuliana Franzosi, Pietro Cesti
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Patent number: 5612210Abstract: The present invention relates to a process for producing large amounts of 7.beta.-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture.Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium.The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7.beta.-(4-carboxybutanamido)-cephalosporanic acid.Type: GrantFiled: December 30, 1994Date of Patent: March 18, 1997Assignee: Ministero Dell'Universita' e Della Ricerca Scientifica e TecnologicaInventors: Wilhelmus Van der Goes, Antonella Bernardi, Aldo Bosetti, Pietro Cesti, Giuliana Franzosi
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Patent number: 5332663Abstract: 7-amino-cephalosporanic acids having the formula ##STR1## are obtained by submitting compounds having the following formula to enzymatic conversion: ##STR2## wherein R represents H, OH, O--CO--R", R" being an alkyl radical with from 1 to 4 carbon atoms, R.sup.1 represents a carboxylic group. The reaction is carried out in the presence of a microorganism selected from the Pseudomonas, Bacillus, Achromobacter genera, or in the presence of an enzyme, either free or immobilized, derived therefrom.Type: GrantFiled: July 14, 1992Date of Patent: July 26, 1994Assignee: Ministero dell'Universita' e della Ricerca Scientifica e TecnologicaInventors: Ezio Battistel, Pietro Cesti, Giuliana Franzosi, Vilhelmus van der Goes
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Patent number: 5302528Abstract: A process is described for the separation of the optical isomers of alphayl- or alpha-aryloxy- carboxylic acids by means of the stereoselective hydrolysis of racemic alkyl esters of the above acids in the presence of bacteria or their enzymes, selected from the species Brevibacterium, Bacteridium, Micrococcus, and Bacillus.Type: GrantFiled: April 22, 1992Date of Patent: April 12, 1994Assignee: Ministero Dell `Universita` e Della Ricerca Scientifica e TechnologicaInventors: Ezio Battistel, Daniele Bianchi, Pietro Cesti, Giuliana Franzosi, Roberto Tassinari, Sandro Spezia