Abstract: Fluorescence microscopy method comprising: illuminating a scattering sample with a coherent excitation light beam having a wavefront with an initial configuration for exciting fluorescent emitters in the scattering sample; acquiring an initial image; selecting target pixels in an area of the initial image; optimising the initial configuration of the wavefront for each target pixel for decreasing the speckle grain size and obtaining a final image; subtracting the initial image from the final image for each target pixel, obtaining an image; fitting the image with a Gaussian function with free center coordinates for each target pixel; generating an image containing a Gaussian distribution centered at the coordinates and intensity, and with a waist equal to an average size S of speckle grain of the scattering sample, for each target pixel; and generating a final image of the sample by summing the images of the target pixels.

Abstract: Spectrometer for analyzing the spectrum of a Brillouin scattered light including input means receiving the scattered light, and selecting means for selecting and separating specific multiple frequency components of the scattered light. The selecting means has at least one main input, and at least an optical detector is coupled to the selecting means for measuring the intensity of the different frequency components and reconstructing the spectrum profile of the scattered light. The selecting means include an optical integrated circuit having at least one optical ring resonator of a first type having an input waveguide for receiving the light from the input means, a closed loop waveguide having an effective refractive index neff, and an output waveguide. The selecting means further have at least a modulator element of the effective refractive index neff coupled to the closed loop waveguide of the optical ring resonator of the first type.

Abstract: Spectrometer for analyzing the spectrum of a Brillouin scattered light including input means receiving the scattered light, and selecting means for selecting and separating specific multiple frequency components of the scattered light. The selecting means has at least one main input, and at least an optical detector is coupled to the selecting means for measuring the intensity of the different frequency components and reconstructing the spectrum profile of the scattered light. The selecting means include an optical integrated circuit having at least one optical ring resonator of a first type having an input waveguide for receiving the light from the input means, a closed loop waveguide having an effective refractive index neff, and an output waveguide. The selecting means further have at least a modulator element of the effective refractive index neff coupled to the closed loop waveguide of the optical ring resonator of the first type.

Abstract: A spectrometer is provided which comprises a Virtually Imaged Phased Array member configured to receive an electromagnetic input radiation and to generate an electromagnetic output radiation, in which a spectrum of the electromagnetic output radiation is dispersed along a dispersion axis (x) transverse to an optical axis (z) of propagation of the electromagnetic output radiation; a Fourier lens adapted to convert the output electromagnetic radiation into a spectral pattern; an image sensor adapted to detect the spectral pattern; and at least one diffraction mask arranged along the optical axis (z) for propagating the output electromagnetic radiation, in a position not coincident with a spectral plane of the spectrometer. Through the diffraction mask, which comprises a material blocking the transmission of the output electromagnetic radiation, an aperture is obtained which allows the transmission of the output electromagnetic radiation, and whose edge comprises at least one inclined segment.

Abstract: Fluorescence microscopy method comprising: illuminating a scattering sample with a coherent excitation light beam having a wavefront with an initial configuration for exciting fluorescent emitters in the scattering sample; acquiring an initial image; selecting target pixels in an area of the initial image; optimising the initial configuration of the wavefront for each target pixel for decreasing the speckle grain size and obtaining a final image; subtracting the initial image from the final image for each target pixel, obtaining an image; fitting the image with a Gaussian function with free center coordinates for each target pixel; generating an image containing a Gaussian distribution centered at the coordinates and intensity, and with a waist equal to an average size S of speckle grain of the scattering sample, for each target pixel; and generating a final image of the sample by summing the images of the target pixels.

Abstract: A spectrometer is provided which comprises a Virtually Imaged Phased Array member configured to receive an electromagnetic input radiation and to generate an electromagnetic output radiation, in which a spectrum of the electromagnetic output radiation is dispersed along a dispersion axis (x) transverse to an optical axis (z) of propagation of the electromagnetic output radiation; a Fourier lens adapted to convert the output electromagnetic radiation into a spectral pattern; an image sensor adapted to detect the spectral pattern; and at least one diffraction mask arranged along the optical axis (z) for propagating the output electromagnetic radiation, in a position not coincident with a spectral plane of the spectrometer. Through the diffraction mask, which comprises a material blocking the transmission of the output electromagnetic radiation, an aperture is obtained which allows the transmission of the output electromagnetic radiation, and whose edge comprises at least one inclined segment.