Patents by Inventor Gjalt Huisman

Gjalt Huisman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Engineered imine reductases and methods for the reductive animation of ketone and amine compounds
    Patent number: 9193957
    Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: May 9, 2013
    Date of Patent: November 24, 2015
    Assignee: Codexis, Inc.
    Inventors: Haibin Chen, Steven J. Collier, Jovana Nazor, Joly Sukumaran, Derek Smith, Jeffrey C. Moore, Gregory Hughes, Jacob Janey, Gjalt Huisman, Scott Novick, Nicholas Agard, Oscar Alvizo, Gregory Cope, Wan Lin Yeo, Stefanie Ng Minor
  • Enone reductases
    Patent number: 8883475
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: October 23, 2012
    Date of Patent: November 11, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
  • Polynucleotides encoding engineered ketoreductase polypeptides
    Patent number: 8877475
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
    Type: Grant
    Filed: September 11, 2012
    Date of Patent: November 4, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • Ketoreductase polypeptides for the production of (S,E)-methyl 2-(3-(3-(2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-hydroxypropyl)benzoate
    Patent number: 8617853
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: December 31, 2013
    Assignee: Codexis, Inc.
    Inventors: Jack Liang, Birthe Borup, Vesna Mitchell, Emily Mundorff, James Lalonde, Gjalt Huisman
  • Engineered imine reductases and methods for the reductive animation of ketone and amine compounds.
    Publication number: 20130302859
    Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Application
    Filed: May 9, 2013
    Publication date: November 14, 2013
    Applicant: CODEXIS,INC.
    Inventors: CHEN Haibin, Steven J. Collier, Jovana NAZOR, Joly SUKUMARAN, Derek SMITH, Jeffrey C. MOORE, Gregory HUGHES, Jacob JANEY, Gjalt HUISMAN, Scott NOVICK, Nicholas AGARD, Oscar ALVIZO, Gregory COPE, Wan Lin YEO, Stephanie NG
  • Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
    Patent number: 8426178
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2- thienyl)-1-propanamine.
    Type: Grant
    Filed: August 27, 2009
    Date of Patent: April 23, 2013
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • POLYNUCLEOTIDES ENCODING ENGINEERED KETOREDUCTASE POLYPEPTIDES
    Publication number: 20130005017
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
    Type: Application
    Filed: September 11, 2012
    Publication date: January 3, 2013
    Applicant: CODEXIS, INC.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • Enone reductases
    Patent number: 8329438
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: December 23, 2009
    Date of Patent: December 11, 2012
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
  • Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
    Patent number: 8288141
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
    Type: Grant
    Filed: August 27, 2009
    Date of Patent: October 16, 2012
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • EVOLUTION OF WHOLE CELLS AND ORGANISMS BY RECURSIVE SEQUENCE RECOMBINATION
    Publication number: 20120252681
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Application
    Filed: December 2, 2011
    Publication date: October 4, 2012
    Applicant: CODEXIS MAYFLOWER HOLDINGS, LLC
    Inventors: Stephen del Cardayre, Matthew Tobin, Willem P.C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswaran Subramanian, Linda A. Castle, Claus M. Krebber, Steven H. Bass, Ying-Xin Zhang, Tony Cox, Gjalt Huisman, Ling Yuan, Joseph A. Affholter
  • KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF (S,E)-METHYL 2-(3-(3-(2-(7-CHLOROQUINOLIN-2-YL)VINYL)PHENYL)-3-HYDROXYPROPYL)BENZOATE
    Publication number: 20120184000
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Application
    Filed: December 19, 2011
    Publication date: July 19, 2012
    Applicant: CODEXIS, INC.
    Inventors: Jack Liang, Birthe Borup, Vesna Mitchell, Emily Mundorff, James Lalonde, Gjalt Huisman
  • AMIDASES AND METHODS OF THEIR USE
    Publication number: 20120021469
    Abstract: The disclosure relates to engineered amidase polypeptides and processes of using the polypeptides for chiral resolution of amino acid amide compounds. The disclosure further relates to the polynucleotides that encode the engineered amidase polypeptides and related vectors, host cells, and methods for making the engineered amidase polypeptides.
    Type: Application
    Filed: March 26, 2010
    Publication date: January 26, 2012
    Applicant: CODEXIS, INC.
    Inventors: Owen Gooding, Robert J. Jones, Gjalt Huisman, Jie Yang, Louis Clark
  • EVOLUTION OF WHOLE CELLS AND ORGANISMS BY RECURSIVE SEQUENCE RECOMBINATION
    Publication number: 20110190140
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Application
    Filed: October 15, 2009
    Publication date: August 4, 2011
    Applicant: Maxygen, Inc.
    Inventors: Stephen del Cardayre, Matthew Tobin, Willem P.C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswatan Subramanian, Linda A. Castle, Claus M. Krebber, Steven H. Bass, Ying-Xin Zhang, Tony Cox, Gjalt Huisman, Ling Yuan, Joseph A. Affholter
  • Ketoreductase polypeptides for the production of ()-3-hydroxythiolane
    Patent number: 7977078
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
    Type: Grant
    Filed: August 24, 2008
    Date of Patent: July 12, 2011
    Assignee: Codexis, Inc.
    Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Rama Voladri, James Lalonde, Gjalt Huisman
  • ENONE REDUCTASES
    Publication number: 20100190218
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Application
    Filed: December 23, 2009
    Publication date: July 29, 2010
    Applicant: CODEXIS, INC.
    Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
  • KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF 3-ARYL-3-HYDROXYPROPANAMINE FROM A 3-ARYL-3-KETOPROPANAMINE
    Publication number: 20100173369
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
    Type: Application
    Filed: August 27, 2009
    Publication date: July 8, 2010
    Applicant: CODEXIS, INC.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF A 3-ARYL-3-HYDROXYPROPANAMINE FROM A 3-ARYL-3-KETOPROPANAMINE
    Publication number: 20100151534
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
    Type: Application
    Filed: August 27, 2009
    Publication date: June 17, 2010
    Applicant: CODEXIS, INC.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives
    Publication number: 20070161094
    Abstract: The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives The present invention also provides methods and compositions for preparing vicinal cyano, hydroxyl substituted carboxylic acid esters.
    Type: Application
    Filed: August 10, 2006
    Publication date: July 12, 2007
    Applicant: Codexis, Inc.
    Inventors: S. Davis, John Grate, David Gray, John Gruber, Gjalt Huisman, Steven Ma, Lisa Newman, Roger Sheldon, Li A. Wang
  • Evolution of whole cells and organisms by recursive sequence recombination
    Publication number: 20070048775
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Application
    Filed: August 17, 2006
    Publication date: March 1, 2007
    Inventors: Stephen Cardayre, Matthew Tobin, Willem Stemmer, Jon Ness, Jeremy Minshull, Phillip Patten, Venkiteswaran Subramanian, Linda Castle, Claus Krebber, Steven Bass, Ying-Xin Zhang, Tony Cox, Gjalt Huisman, Ling Yuan, Joseph Affholter
  • Evolution of whole cells and organisms by recursive sequence recombination
    Publication number: 20070026446
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Application
    Filed: August 17, 2006
    Publication date: February 1, 2007
    Inventors: Stephen del Cardayre, Matthew Tobin, Willem Stemmer, Jon Ness, Jeremy Minshull, Phillip Patten, Venkiteswaran Subramanian, Linda Castle, Claus Krebber, Steven Bass, Ying-Xin Zhang, Tony Cox, Gjalt Huisman, Ling Yuan, Joseph Affholter