Abstract: Systems, methods, compositions, and the like, such as diagnostics, medicaments, and therapeutics, etc., relating to Neuropeptide FF2 Receptor, also known as GPR74. Such diagnostics and therapeutics, etc., include for example the nucleic acids, peptides, proteins, etc., of GPR74, as well as antibody and nucleic acid based compositions, treatments, assays, agonists, antagonists, probes, antisense, and gene therapies. The diagnosis, treatment, inhibition, etc., can be, for example, of Alzheimer's disease, of diseases of myocytes, such as myocardial infarct and diabetes-related conditions and congestive heart failure, and of Parkinson's disease.

Abstract: This invention relates to the discovery of nucleic acids and proteins associated with aging processes. The identification of these aging-associated nucleic acids and proteins have diagnostic uses in detecting the aging status of a cell population as well as application for treating or delaying cellular and physiological changes that occur with aging or the onset of diseases typically associated with aging.

Abstract: The present invention comprises systems, methods, compositions and the like, such as diagnostics, medicaments and therapeutics, relating to GPR 38 and Alzheimer's disease and Parkinson's disease, inflammatory bowel diseases including ulcerative colitis and Crohn's disease, Hodgkin's disease, glioblastoma and carcinomas including breast, colon, lung (small cell and adenocarcinoma) pancreatic (small cell and adenocarcinoma), ovarian, and prostate. Such diagnostics and therapeutics include peptide, protein, antibody and nucleic acid based compositions, including agonists, antagonists, probes, antisense and gene therapy compositions.

Abstract: Diagnostics, therapeutics and the like based on anaphylatoxin C3a receptor directed against Alzheimer's disease and Parkinson's disease. The compositions and methods and the like can include one or more of peptide, protein, antibody, and nucleic acid components, and can be useful, for example, as agonists, antagonists, probes, antisense and gene therapy compositions and otherwise as may be desired.

Abstract: Systems, methods, compositions, and the like, such as diagnostics, medicaments, and therapeutics relating to CCXCR1 and allergic rhinitis, rheumatoid arthritis, cancers including ovarian, colonic, pancreatic, and prostatic carcinoma, and wound healing. Such diagnostics and therapeutics include peptide, protein, antibody, and nucleic acid based compositions, including agonists, antagonists, probes, antisense, and gene therapy compositions.

Abstract: The present invention provides antigenic peptides for GPCRs and antibodies relating thereto, and related systems, methods, compositions, and the like, such as diagnostics and medicaments. Where antibodies against a given GPCR are not known, the present invention provides such antibodies, and preferred antigenic sequences for producing such antibodies. Where antibodies against a given GPCR are known, the present invention provides preferred antigenic peptides for producing antibodies that exhibit improved specificity, affinity or capacity to perform antibody-related actions relative to the known antibodies.

Abstract: This invention relates to the discovery of nucleic acids associated with cell proliferation, cell cycle arrest, cell death and premature aging and uses therefor.

Abstract: An expert system and software method for image recognition optimized for the repeating patterns characteristic of organic material. The method is performed by computing parameters across a two dimensional grid of pixels (rather than a one dimensional scan) with intensity values for each pixel having precision of eight significant bits. The parameters are fed to multiple neural networks, one for each parameter, which were each trained with images showing the tissue, structure, or nucleus to be recognized and trained with images likely to be presented that do not include the material to be recognized. Each neural network then outputs a measure of similarity of the unknown material to the known material on which the network was trained. The outputs of the multiple neural networks are aggregated by an associative voting matrix. A sub-neural network is used for each identified mode of data degradation in the input data.

Abstract: This invention relates to the discovery of the association of certain nucleic acid sequences and proteins with breast cancer, the use of such sequences as a diagnostic indicator and treatments based on the association.

Abstract: The present invention relates to the discovery of nucleic acids and proteins that are markers for specific normal or cancerous tissues. The identification of these markers has diagnostic uses in detecting cancer in a subject, as well as in determining the tissue of origin for a cancerous cell. These tissue-specific molecules further allow the tissue specific expression of a polynucleotide or protein of interest. In addition, the tissue-specific molecules of the invention have therapeutic applications for targeting compounds to a tissue of interest.

Abstract: This invention relates to the discovery of nucleic acids and proteins associated with the aging processes, such as cell proliferation and senescence, and in particular with skin aging. The identification of these aging-associated nucleic acids and proteins have diagnostic uses in detecting the aging status of a cell population as well as application for gene therapy and the delaying of the aging process.

Abstract: The described invention includes methods and assays to identify compounds that interact with a binding domain of a protein by a competition assay with a labeled ligand targeted to the peptides of the binding domain of that protein. These assays can be used for high throughput compound screening, or used to identify sites of compound interaction with proteins in tissue samples.

Abstract: This invention provides methods for the isolation of complete representation of cellular messenger ribonucleic acid from wax-embedded tissue samples. Expression of genes of interest can thus be fortuitously determined and cDNA probes can be readily developed.

Abstract: The present invention relates generally to high throughput screening methods. More particularly, the present invention provides screening methods that can readily be used to identify simultaneously multiple proteins or compounds that interact with multiple ligands, using a tagged array of ligands.

Abstract: The present invention provides gene subtraction methods that can be used to identify and isolate from hybridization mixtures differentially expressed nucleic acid and/or commonly expressed nucleic acid between two populations of nucleic acid. The methods of the present invention employ a restriction endonuclease recognition site that is also recognized in whole or in part by a second restriction endonuclease (e.g., HinPI and BssHII or HhaI and HinP1I) to create different ends (e.g., sticky ends) between the first and second nucleic acid populations. In addition, the methods of the present invention use a selective ligation step, a biotinylated nucleotide extension step, or both to differentiate between homoduplexes, which allow for the identification of sequences that are different between the two nucleic acid populations, and heteroduplexes, which allow for the identification of sequences that are common to the two nucleic acid populations.

Abstract: The invention disclosed here allows for the differential isolation of nucleic acid sequences that are present in one nucleic acid population and not in another. The method is based upon using restriction endonucleases to digest two populations of nucleic acid, preferably cDNAs, preferably ligating different sets of adaptors to each of the two nucleic acid populations, followed by hybridization, restriction digestion and isolation of the desired molecules. The unique aspects of this invention include the use of a restriction enzyme to isolate the target duplex DNA molecule from a hybridization mixture. Certain embodiments of the invention include the direct or indirect incorporation of a capture molecule or ligand (e.g., biotin, dioxigenin) within the amplified nucleic acid fragments, which allows for a system in which molecules can be rescued from both the captured population as well as the effluent or otherwise uncaptured population.