Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

Abstract: The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. In accordance with the present invention, a selectable marker gene has been recombinantly targeted to the brachyury locus to allow the isolation and characterization of cell populations that comprise brachyury positive mesendoderm and mesoderm cells. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Abstract: The invention provides a method for generating a transgenic eukaryotic cell population having a modified human Rosa26 locus, which method includes introducing a functional DNA sequence into the human Rosa26 locus of starting eukaryotic cells. Also provided are targeting vectors useful in the method, as well as a cell population and a transgenic non-human animal comprising a modified human Rosa26 locus. Finally, the invention provides an isolated DNA sequence corresponding to the human Rosa26 locus.

Abstract: A cover for an empty rail missile launcher that can be used in flight. The cover is shaped to reflect radar signals transmitted by a radar transmitter away from the radar transmitter to reduce detectibility by radar. The cover may also be coated with radar absorbent material to reduce detectibility by radar. Hangers are used to mount the cover to the rail missile launcher. The cover is provided with a grounding mechanism to dissipate precipitation static. A restraint mechanism is provided to prevent the cover from inadvertently sliding off the rail missile launcher.

Abstract: A cover for an empty rail missile launcher that can be used in flight. The cover is shaped to reflect radar signals transmitted by a radar transmitter away from the radar transmitter to reduce detectibility by radar. The cover may also be coated with radar absorbent material to reduce detectibility by radar. Hangers are used to mount the cover to the rail missile launcher. The cover is provided with a grounding mechanism to dissipate precipitation static. A restraint mechanism is provided to prevent the cover from inadvertently sliding off the rail missile launcher.

Abstract: Methods and compositions for producing NKX6-1+ pancreatic progenitor cells and/or insulin producing cells from an endodermal cell population, the method comprising contacting the endodermal cell population with an EGF component, a Nicotinamide component and/or a Noggin component, optionally a combination of at least one EGF component and at least one nicotinamide component to induce the differentiation of at least one endodermal cell into a NKX6-1+ pancreatic progenitor cell, the combination optionally further comprising at least one Noggin component.

Abstract: There is described herein a method of enriching a population of stem cells for hematopoietic progenitors. The method comprises inducing hematopoietic differentiation in a population of human embryonic stem cells or human induced pluripotent stem cells; sorting the population based on expression of CD43 and at least one of CD34, CD31 and CD144; and selecting a fraction that is at least one of CD34+CD43?, CD31+CD43? and CD144+CD43?. Also provided are populations of hematopoietic progenitors obtained by the methods described herein.

Abstract: The present invention relates to methods for enriching pluripotent stem cell-derived cardiomyocyte progenitor cells and cardiomyocyte cells based on SIRPA expression.

Abstract: The present invention provides methods for inducing the differentiation of cardiac progenitor cells and cell populations produced by the methods of the invention. The invention further provides a method of screening for agents that affect cardiomyocytes, and a method of cardiomyocyte replacement therapy.

Abstract: The invention provides a method for generating a transgenic eukaryotic cell population having a modified human Rosa26 locus, which method includes introducing a functional DNA sequence into the human Rosa26 locus of starting eukaryotic cells. Also provided are targeting vectors useful in the method, as well as a cell population and a transgenic non-human animal comprising a modified human Rosa26 locus. Finally, the invention provides an isolated DNA sequence corresponding to the human Rosa26 locus.

Abstract: The present invention provides methods for adenoassociated virus-mediated site-specific integration of a transgene into a stem cell. Stem cells having a transgene integrated therein, and differentiated cells generated from the stem cells are also provided.

Abstract: The present invention provides populations of human cardiovascular progenitor cells, methods of making such cells, and methods of using the cells for production of populations of cardiovascular colonies and populations of cardiomyocytes. Methods of cardiomyocytes replacement therapy are also provided.

Abstract: The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Abstract: An in vitro method for the differentiation of functionally mature neutrophils from stem cells is disclosed. Also disclosed are methods of producing genetically modified neutrophils in vitro and methods of using the neutrophils produced according to the invention.

Abstract: The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Abstract: The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Abstract: A wireless remote controlled mirror having lights to illuminate a subject being viewed in the mirror, while enabling a user to selectively adjust a view of the subject from a remote location. When attached to a rear seat of a vehicle, the mirror can be remotely adjusted to view a child in a rear-facing safety seat. The mirror includes a base adapted to mount to an object, a reflective lens that moves relative to the base, one or more electric motors to drive the reflective lens, and a receiver that detects a command signal from a wireless remote controller and energizes a motor to reorient the reflective lens to view the child. A plurality of light sources are selectively energized to emit light toward the subject, so that the mirror is usable after dark. The mirror can alternatively be mounted on other portions of a vehicle.

Abstract: A wireless remote controlled mirror enabling a user to selectively adjust a view from a remote location. A preferred embodiment enables a driver to remotely control a mirror attached to a rear seat of a vehicle to remotely adjust the view of a child in a rear-facing safety seat. A preferred embodiment includes a base that mounts to an object (such as the safety seat or the rear seat), a reflective lens that moves relative to the base, one or more electric motors to drive the reflective lens, and a receiver that detects a command signal from a wireless remote controller and energizes the one or more motors to reorient the reflective lens to view the child. The mirror can alternatively be mounted on other portions of a vehicle, e.g., as a remote controlled side mirror.

Abstract: A wireless remote controlled mirror having lights to illuminate a subject being viewed in the mirror, while enabling a user to selectively adjust a view of the subject from a remote location. When attached to a rear seat of a vehicle, the mirror can be remotely adjusted to view a child in a rear-facing safety seat. The mirror includes a base adapted to mount to an object, a reflective lens that moves relative to the base, one or more electric motors to drive the reflective lens, and a receiver that detects a command signal from a wireless remote controller and energizes a motor to reorient the reflective lens to view the child. A plurality of light sources are selectively energized to emit light toward the subject, so that the mirror is usable after dark. The mirror can alternatively be mounted on other portions of a vehicle.

Abstract: A wireless remote controlled mirror enabling a user to selectively adjust a view from a remote location. A preferred embodiment enables a driver to remotely control a mirror attached to a rear seat of a vehicle to remotely adjust the view of a child in a rear-facing safety seat. A preferred embodiment includes a base that mounts to an object (such as the safety seat or the rear seat), a reflective lens that moves relative to the base, one or more electric motors to drive the reflective lens, and a receiver that detects a command signal from a wireless remote controller and energizes the one or more motors to reorient the reflective lens to view the child. The mirror can alternatively be mounted on other portions of a vehicle, e.g., as a remote controlled side mirror.