Patents by Inventor Gordon Nye

Gordon Nye has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for measuring cysteine and determining cysteine synthase activity
    Patent number: 6605459
    Abstract: Assays, preferably high throughput assays, for determining cysteine concentration, cysteine synthase activity, and identifying herbicides, fungicides, bactericides, and insecticides. Cysteine concentration is quantitated by contacting cysteine with a coumarin dye capable of conjugating with cysteine but not to O-acetyl serine or sulfide; exciting the conjugate with UV light; and detecting fluorescent light emitted by the conjugate. Cysteine synthase activity is determined by combining O-acetyl-L-serine, sulfide and cysteine synthase to form a reaction mixture under conditions suitable for cysteine production; contacting the reaction mixture with an appropriate coumarin dye; subjecting the reaction mixture to UV light; and detecting fluorescent light emission.
    Type: Grant
    Filed: July 13, 2001
    Date of Patent: August 12, 2003
    Assignee: Paradigm Genetics, Inc.
    Inventors: John Rice, Beth Lanning, John Crawford, Gordon Nye
  • Methods for measuring cysteine and determining cysteine synthase activity
    Publication number: 20030087327
    Abstract: Assays, preferably high throughput assays, for determining cysteine concentration, cysteine synthase activity, and identifying herbicides, fungicides, bactericides, and insecticides. Cysteine concentration is quantitated by contacting cysteine with a coumarin dye capable of conjugating with cysteine but not to O-acetyl serine or sulfide; exciting the conjugate with UV light; and detecting fluorescent light emitted by the conjugate. Cysteine synthase activity is determined by combining O-acetyl-L-serine, sulfide and cysteine synthase to form a reaction mixture under conditions suitable for cysteine production; contacting the reaction mixture with an appropriate coumarin dye; subjecting the reaction mixture to UV light; and detecting fluorescent light emission.
    Type: Application
    Filed: July 13, 2001
    Publication date: May 8, 2003
    Inventors: John Rice, Beth Lanning, John Crawford, Gordon Nye