Patents by Inventor Goushi Ishihara

Goushi Ishihara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector
    Patent number: 7253144
    Abstract: We claim a method of expression a thioredoxin-fused membrane protein comprising expressing said thioredoxin-fused membrane protein in a cell-free protein synthesis system in the presence of a non-ionic detergent. It is preferable that the fused membrane protein which is a highly hydrophobic protein, e.g., a G protein-coupled receptor, can be produced in a state being highly soluble and capable of forming a biologically active three-dimensional structure. Also, this invention provides a recombinant polynucleotide encoding said thioredoxin-fused membrane protein which is expressed in the cell-free protein synthesis system.
    Type: Grant
    Filed: November 19, 2004
    Date of Patent: August 7, 2007
    Assignee: Riken
    Inventors: Mikako Shirouzu, Goushi Ishihara, Mihoro Saeki, Mie Goto, Kaori Tajima, Takanori Kigawa, Shigeyuki Yokoyama
  • Process for synthesizing protein using cell-free protein synthesis system
    Patent number: 7045593
    Abstract: A method for producing a protein using a cell-free protein synthesis system comprising a detergent so that the protein can be synthesized without aggregation, is provided. The protein is a protein comprising a hydrophobic region in at least a portion thereof, for example, a membrane protein or its fragment (portion). And the detergent is a mild detergent which would not denature the protein, for example, a nonionic or amphoteric ionic detergent.
    Type: Grant
    Filed: April 26, 2002
    Date of Patent: May 16, 2006
    Assignee: Riken
    Inventors: Kaori Tajima, Takanori Kigawa, Mikako Shirouzu, Takashi Yabuki, Goushi Ishihara, Shigeyuki Yokoyama
  • Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector
    Publication number: 20050255542
    Abstract: A fusion protein of thioredoxin with a target protein is expressed in a cell-free protein synthesis system to thereby synthesize at least a part of the fusion protein as a soluble protein. It is preferable that the target protein is a highly hydrophobic protein such as a membrane protein. In particular, a membrane protein such a G protein-coupled receptor can be produced in a state being highly soluble and capable of forming a biologically active three dimensional structure. Also, provided a recombinant vector for producing such a membrane protein in a cell-free protein synthesis system.
    Type: Application
    Filed: November 19, 2004
    Publication date: November 17, 2005
    Applicant: RIKEN
    Inventors: Mikako Shirouzu, Goushi Ishihara, Mihoro Saeki, Mie Goto, Kaori Tajima, Takanori Kigawa, Shigeyuki Yokoyama
  • Process for synthesizing protein using cell-free protein synthesis system
    Publication number: 20040203091
    Abstract: A method for producing a protein using a cell-free protein synthesis system comprising a detergent so that the protein can be synthesized without aggregation, is provided. The protein is a protein comprising a hydrophobic region in at least a portion thereof, for example, a membrane protein or its fragment (portion). And the detergent is a mild detergent which would not denature the protein, for example, a nonionic or amphoteric ionic detergent.
    Type: Application
    Filed: December 30, 2003
    Publication date: October 14, 2004
    Inventors: Kaori Tajima, Takanori Kigawa, Mikako Shirouzu, Takashi Yabuki, Goushi Ishihara, Shigeyuki Yokoyama