Abstract: The present invention is directed to a chromatography device with a stationary phase containing multiple binding moieties. The binding moieties are first solubilized and then immobilized on a stationary phase to create a multiple binding moieties phase for use in a chromatography device. In an alternative to the stationary phase embodiment, a single binding moiety can be directly bonded covalently to a support within the chromatography column. Combinations of constructions involving stationary phase immobilization and direct covalent bonding can also be employed. The multiple binding moiety chromatography devices are useful in investigating interactions among different binding moieties in pharmacological studies and in drug discovery.