Abstract: Compositions and methods are provided for diagnosis, prognosis, and/or monitoring of Kawasaki disease or MIS-C (e.g., associated with SARS-CoV-2 infection) in a subject. In some embodiments, the method includes measuring, comparing and weighting the level of particular proteins to other proteins. In other embodiments, the method includes comparison with clinical variable information.

Abstract: Compositions and methods are provided for diagnosis and/or prognosis of peripheral artery disease, aortic stenosis, or cardiovascular events in a subject. In some embodiments, the method includes measuring and comparing the level of particular proteins to other proteins. In other embodiments, the method includes comparison with clinical variable information.

Abstract: Compositions and methods are provided for diagnosis and/or prognosis of acute kidney injury risk following medical procedures in a subject. In some embodiments, the method includes measuring and comparing the level of particular proteins to other proteins. In other embodiments, the method includes measuring proteins levels with clinical variable information and comparing this composite with the composite of other protein levels with clinical variable information.

Abstract: The embodiments disclosed relate to determination of an item of interest in a sample. One embodiment relates to a structure which comprises a process path. The process path comprises a process lane including a process step performance lane where a process step is performed, and a process step avoidance lane where the process step is avoided. A first prime mover is operatively connected with the process path for moving a container holding the sample along the process path. A first pipetting system is operatively associated with the process path for introducing the sample to the container. A second pipetting system is operatively associated with the process path for introducing a reagent to the container. A device is operatively connected with the process path and is selectively engagable with the container for mixing the sample and the reagent in the container.

Abstract: The embodiments disclosed relate to determination of an item of interest in a sample. In one method, a process path comprising a process lane including a process step performance lane where a process step is performed, and a process step avoidance lane where the process step is avoided is provided. A container holding the sample is moved along the process path. The sample is introduced to the container. A reagent is introduced to the container. The sample and the reagent are mixed in the container. The container is selectively positioned in a selected one of the process step performance lane and the process step avoidance lane. The item of interest in the sample is determined based upon a reaction between the sample and the reagent.

Abstract: Embodiments described herein provide methods of performing a process for determining an item of interest in a sample. In one embodiment, a container for holding the sample is accepted in a process lane where a process step is selectively automatically performed on the sample in the container. The process step is selectively automatically performed on the sample in the container. An effective length of the process lane is maintained constant while a physical length of the process lane is selectively varied.

Abstract: Novel methods for covalent attachment of antibodies, antigens, or other molecules to solid phases using extended length heterobifunctional crosslinking reagents are disclosed. The resulting derivatized solid phases can be used in diagnostic assays.

Abstract: Novel polyamino acid based coupling agents are disclosed. These reagents are useful for conjugating proteins (e.g. antibodies to enzymes) for use in diagnostic assays.

Abstract: Novel methods for covalent attachment of antibodies, antigens, or other molecules to solid phases using extended length heterobifunctional crosslinking reagents are disclosed. The resulting derivatized solid phases can be used in diagnostic assays.

Abstract: Novel polyamino acid based coupling agents are disclosed. These reagents are useful for conjugating proteins (e.g. antibodies to enzymes) for use in diagnostic assays.