Abstract: The present proposals relate to methods for detection of whole-cell bacteria, for example whole-cell pathogenic bacteria, in particular drug-resistant bacteria. These proposals also include apparatus and kits for use in such methods.

Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of:

Abstract: Disclosed is a method of detecting a nucleic acid sequence of interest in a sample, the method comprising contacting the sample with first and second probes; the first probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest; the second probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest but complementary to that portion of the first probe which is non-complementary to the sequence of interest, such that the first and second probes are capable of hybridising to the sequence of interest in an adjacent or substantially adjacent manner, so as to allow complementary portions of the first and second probes to hybridize to each other; causing extension of the first probe with a nucleic acid polymerase, using the second probe as a template; and detecting directly or indirectly the extension of

Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of: (a) adding first and second nucleic acid probes to a sample comprising the sequence of interest, so as to form a complex comprising three strands of nucleic acid, wherein the first probe comprises the full length sequence of a first strand of a double stranded promoter, the target sequence comprises an end part of a second strand of the double stranded promoter which is complementary to a part of the first strand, and the second probe comprises the rest of the second strand of the double stranded promoter which is complementary to a part of the first strand, such that a functional promoter is formed when the first probe is hybridized to both the target sequence and to the second probe; (b) adding a polymerase which recognizes the promoter, so as to cause the de novo synthesis of nucleic acid from the promoter present in the complex; and (c) detecting directly or indirectly the

Abstract: An oligonucleotide containing at least one cytidine analog labeled at C4 with a reporter group.

Abstract: An oligonucleotide containing at least one cytidine analog labeled at C4 with a reporter group.

Abstract: Polynucleotides are labeled with chemiluminescent acridine esters or luminescent lanthanides. Labelling is preferably carried out by incorporating a functional group into a nucleotide or polynucleotide at the C4 position of the pyrimidine portion or the C6 position of the purine portion and bonding a chemiluminescent acridine ester or a luminescent lanthanide to the functional group. The labeled polynucleotides are useful for direct detection of homologous polynucleotide sequences.