Abstract: A method for preparing an adsorptive media for binding biologic molecules comprising immersing a macroporous support in a first solution of a coupling reagent in a solvent solution for attachment of said coupling reagent to form coupling groups; and, immersing said macroporous support in an incubating solution selected from the group consisting of ligand, nucleotide, oligonucleotide, peptide, polypeptide, protein, and enzyme solutions having an affinity to a biologic target molecule to couple one of said ligands, nucleotides, oligonucleotides, peptides, polypeptides, proteins, and enzymes to at least a portion of said coupling groups of said macroporous support for binding with said biologic target molecule when exposed to said macroporous support.

Abstract: A method for preparing an adsorptive media for binding biologic molecules comprising immersing a macroporous support in a first solution of a coupling reagent in a solvent solution for attachment of said coupling reagent to form coupling groups; and, immersing said macroporous support in an incubating solution selected from the group consisting of ligand, nucleotide, oligonucleotide, peptide, polypeptide, protein, and enzyme solutions having an affinity to a biologic target molecule to couple one of said ligands, nucleotides, oligonucleotides, peptides, polypeptides, proteins, and enzymes to at least a portion of said coupling groups of said macroporous support for binding with said biologic target molecule when exposed to said macroporous support.

Abstract: Separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity group capable of binding to a peptide purification tag on a target protein. Methods of making the separation media and methods of using the separation media are disclosed.

Abstract: Separation media and separation devices containing the same are disclosed. Separation media includes a support substrate and a plurality of separation ligands. The separation ligands include an affinity group capable of binding a metal, capable of binding an enantiomer of a chiral molecule, or both. Methods of using the separation media are also disclosed.

Abstract: Separation media that includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of binding to a diol of a target molecule. The affinity group includes a boronic acid or a benzoxaborole. Methods of making the separation media and methods of using the separation media.

Abstract: Separation media containing dyes is disclosed. Separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity group capable including a dye. The affinity group is capable of binding a biomolecule. Methods of making the separation media and methods of using the separation media are disclosed.

Abstract: A method for preparing an adsorptive media for binding biologic molecules comprising immersing a macroporous support in a first solution of a coupling reagent in a solvent solution for attachment of said coupling reagent to form coupling groups; and, immersing said macroporous support in an incubating solution selected from the group consisting of ligand, nucleotide, oligonucleotide, peptide, polypeptide, protein, and enzyme solutions having an affinity to a biologic target molecule to couple one of said ligands, nucleotides, oligonucleotides, peptides, polypeptides, proteins, and enzymes to at least a portion of said coupling groups of said macroporous support for binding with said biologic target molecule when exposed to said macroporous support.

Abstract: The present technology relates to a cassette assembly having an inlet cassette plate defining an inlet flow path, and an outlet cassette plate defining an outlet flow path. The outlet cassette plate is configured to be arranged in a stack with the inlet cassette plate. A membrane stack is disposed between the inlet cassette plate and the outlet cassette plate. The inlet flow path is configured to be in fluid communication with the outlet flow path through the membrane stack to form an assembly flow path. The cassette assembly is configured to receive an elution solution, and an elution concentration is substantially constant as the inner nominal volume varies.

Abstract: Separation media that includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of recognizing and binding to blood type antigen recognizing domain. Methos of making the separation media and methods of using the separation media.

Abstract: Separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of recognizing and binding to a carbohydrate recognizing domain. Methods of making the separation media and methods of using the separation media are disclosed.

Abstract: Separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity group capable of binding to a carbohydrate. Methods of making the separation media and methods of using the separation media are disclosed.

Abstract: Methods of using a separation media to isolate a target molecule that includes a carbohydrate are disclosed. The separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of recognizing and binding to a carbohydrate.

Abstract: A method for preparing an adsorptive media for binding biologic molecules comprising immersing a macroporous support in a first solution of a coupling reagent in a solvent solution for attachment of said coupling reagent to form coupling groups; and, immersing said macroporous support in an incubating solution selected from the group consisting of ligand, nucleotide, oligonucleotide, peptide, polypeptide, protein, and enzyme solutions having an affinity to a biologic target molecule to couple one of said ligands, nucleotides, oligonucleotides, peptides, polypeptides, proteins, and enzymes to at least a portion of said coupling groups of said macroporous support for binding with said biologic target molecule when exposed to said macroporous support.

Abstract: Disclosed are methods for purifying polynucleotides from a feed solution using oligonucleotide affinity columns at flowrates between 0.5 CV/min to 1000 CV/min. The methods can include several steps including loading a mixture containing the target polynucleotide onto a chromatography media, e.g., a column, that carries a macroporous support with an oligonucleotide affinity ligand bonded to a surface. The affinity ligand can hybridize the targeted polynucleotide and allow for separation and purification of the target.

Abstract: Separation media includes a membrane and a plurality of ligands immobilized on the membrane, the plurality of ligands comprising anion-exchange ligands, cation-exchange ligands, thiophilic ligands, hydrophilic ligands, hydrophobic interaction ligands, or a combination thereof. The separation media may be multimodal. The separation media may be configured for separation of target molecules comprising a nucleic acid, nucleotide, nucleoside, nucleobase, or an analogue or derivative thereof, from a reaction mixture. The separation media may be configured for use with organic solvents. A separation device includes the separation media. Materials including a nucleic acid, nucleotide, nucleoside, nucleobase, or an analogue or derivative thereof, may be purified at high speeds using the separation device.

Abstract: Disclosed are methods for forming an activated membrane that can be further derivatized for use purifying plasmid DNA using hydrophobic interaction separation methods. Activated membrane and derivatized membrane formed by the methods are also described. HIC systems incorporating the derivatized membrane as described herein can exhibit a high plasmid DNA binding capacity and short residence times.

Abstract: A method for preparing an adsorptive media for binding biologic molecules comprising immersing a macroporous support in a first solution of a coupling reagent in a solvent solution for attachment of said coupling reagent to form coupling groups; and, immersing said macroporous support in an incubating solution selected from the group consisting of ligand, nucleotide, oligonucleotide, peptide, polypeptide, protein, and enzyme solutions having an affinity to a biologic target molecule to couple one of said ligands, nucleotides, oligonucleotides, peptides, polypeptides, proteins, and enzymes to at least a portion of said coupling groups of said macroporous support for binding with said biologic target molecule when exposed to said macroporous support.