Patents by Inventor Grant T. Shimamoto

Grant T. Shimamoto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of somatotropin solubilization using dimethylsulfone and naturation
    Patent number: 4731440
    Abstract: A method for solubilization and naturation of somatotropin protein from refractile bodies of host cells is disclosed. The method embraces the discovery that an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, can be effectively used to solubilize refractile bodies containing such somatotropin protein. Once solubilized, somatotropin protein can be natured in an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide bonds. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.
    Type: Grant
    Filed: August 25, 1986
    Date of Patent: March 15, 1988
    Assignee: Monsanto Company
    Inventors: Larry A. Bentle, James W. Mitchell, Stephen B. Storrs, Grant T. Shimamoto
  • Method of somatotropin solubilization and naturation using dimethylsulfoxide
    Patent number: 4694073
    Abstract: A method for solubilization and naturation of somatotropin protein from refractile bodies of host cells is disclosed. The method embraces the discovery that an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, can be effectively used to solubilize refractile bodies containing such somatotropin protein. Once solubilized, somatotropin protein can be natured in an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide bonds. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.
    Type: Grant
    Filed: August 25, 1986
    Date of Patent: September 15, 1987
    Assignee: Monsanto Company
    Inventors: Larry A. Bentle, James W. Mitchell, Stephen B. Storrs, Grant T. Shimamoto