Patents by Inventor Gregory A. Gambetta

Gregory A. Gambetta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    Publication number: 20080213901
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases.
    Type: Application
    Filed: January 31, 2008
    Publication date: September 4, 2008
    Applicant: Novozymes, Inc.
    Inventors: John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
  • Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    Patent number: 7332341
    Abstract: The present invention relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
    Type: Grant
    Filed: March 31, 2005
    Date of Patent: February 19, 2008
    Assignee: Novozymes, Inc.
    Inventors: John C. Royer, Lynne M. Christanson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
  • HY2 family of bilin reductases
    Patent number: 7033806
    Abstract: This invention identifies a novel family of bilin reductases. Designated herein HY bilin reductases, the enzymes of this invention are useful in a wide variety of contexts including but not limited to the conversion of biliverdins to phytobilins and the assembly of holophytochromes or phytofluors.
    Type: Grant
    Filed: May 29, 2001
    Date of Patent: April 25, 2006
    Assignee: The Regents of the University of California
    Inventors: John Clark Lagarias, Takayuki Kochi, Nicole Frankenberg, Gregory A. Gambetta, Beronda L. Montgomery
  • Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    Publication number: 20050181480
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases.
    Type: Application
    Filed: March 31, 2005
    Publication date: August 18, 2005
    Applicant: Novozymes Biotech, Inc.
    Inventors: John Royer, Lynne Christianson, Gregory Gambetta, Howard Brody, Suzanne Otani, Wendy Yoder
  • Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    Patent number: 6903193
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
    Type: Grant
    Filed: November 10, 2000
    Date of Patent: June 7, 2005
    Assignee: Novozymes Biotech, Inc.
    Inventors: John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
  • Light controlled gene expression utilizing heterologous phytochromes
    Patent number: 6887688
    Abstract: This invention relates to the field of gene expression. In particular this invention relates to the use of heterologous phytochromes to translocate polypeptides into the nucleus of a cell. Where the polypeptides comprise transactivators or repressors this invention provides a system for light-directed gene expression.
    Type: Grant
    Filed: May 29, 2002
    Date of Patent: May 3, 2005
    Assignee: The Regents of the University of California
    Inventors: John Clark Lagarias, Takayuki Kochi, Nicole Frankenberg, Gregory A. Gambetta, Beronda L. Montgomery
  • HY2 family of bilin reductases
    Publication number: 20030104379
    Abstract: This invention identifies a novel family of bilin reductases. Designated herein HY bilin reductases, the enzymes of this invention are useful in a wide variety of contexts including but not limited to the conversion of biliverdins to phytobilins and the assembly of holophytochromes or phytofluors.
    Type: Application
    Filed: May 29, 2001
    Publication date: June 5, 2003
    Applicant: The Regents of the University of California
    Inventors: John Clark Lagarias, Takayuki Kochi, Nicole Frankenberg, Gregory A. Gambetta, Beronda L. Montgomery
  • Light controlled gene expression utilizing heterologous phytochromes
    Publication number: 20030073235
    Abstract: This invention relates to the field of gene expression. In particular this invention relates to the use of heterologous phytochromes to translocate polypeptides into the nucleus of a cell. Where the polypeptides comprise transactivators or repressors this invention provides a system for light-directed gene expression.
    Type: Application
    Filed: May 29, 2002
    Publication date: April 17, 2003
    Applicant: The Regents of the University of California
    Inventors: John Clark Lagarias, Takayuki Kochi, Nicole Frankenberg, Gregory A. Gambetta, Beronda L. Montgomery
  • Methods for producing heterologous polypeptides in trichothecene-deficient
    Patent number: 6180366
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases.
    Type: Grant
    Filed: May 20, 1999
    Date of Patent: January 30, 2001
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder