Abstract: A spatio-temporal profilometer performs time-resolved spatial profilometry and includes a substrate, a tapered optical collimator waveguide, a fluid channel, and a light-fluid interaction volume. The tapered optical collimator waveguide receives diverging light, internally reflects it, and collimates it. The fluid channel receives a fluid comprising microparticles and communicates the microparticles into the fluid channel. The light-fluid interaction volume is disposed in the fluid channel and provided by an overlap within the fluid channel of the collimated light from the tapered optical collimator waveguide and the fluid. The spatio-temporal profilometer produces product light from the collimated light in response to the microparticles interacting with the collimated light in the light-fluid interaction volume from which is determined a spatial and temporal profile of microparticles in the fluid channel.

Abstract: Embodiments of the present invention described herein provide a device that can measure a single particle in flow several times along a microchannel with integrated waveguides that carry optical signals (e.g. excitation, emission, transmission, and scattered light) to and from measurement regions. Embodiments of the present invention used to perform multiple measurements of particles, such as microspheres or cells, traveling in a sample fluid through a microfluidic channel achieve lower uncertainties, discriminate complex samples, and account for sources of uncertainty that might be related to the shape, deformability, stability, or activity of objects in a liquid sample.

Abstract: An optical flow meter includes a substrate; a microchannel with a fluid receiver; a fluid transmitter; a fluid member with an optical interaction region; a photo interaction region; an analytical light path, such that analytical light interacts with an analyte in a photo interaction region subsequent to an interaction of a pre-analyte with activation light in an optical interaction region to produce analyte; and a detection light path disposed in the substrate, arranged at an oblique angle or right angle to the fluid member proximate to the photo interaction region, and that: receives the photoanalyte light from the photo interaction region; and communicates the photoanalyte light from the microchannel to a photodetector, the optical flow meter determines a flow rate of the analyte.

Abstract: A multiplexed amplitude modulation photometer includes a microchannel; a first input light path that: receives a first modulated light at a first modulation frequency; and communicates the first modulated light to a first optical region that receives the first analyte that produces a first output light including the first modulation frequency communicated to a first detection light path; the first optical region; the first detection light path that receives the first output light; a second input light path that: receives a second modulated light with a second modulation frequency; and communicates second modulated light to a second optical region that receives the second analyte that produces a second output light with the second modulation frequency communicated to a second detection light path; the second optical region; and the second detection light path that receives the second output light from the second optical region.

Abstract: An optical flow meter includes a substrate; a microchannel with a fluid receiver; a fluid transmitter; a fluid member with an optical interaction region; a photo interaction region; an analytical light path, such that analytical light interacts with an analyte in a photo interaction region subsequent to an interaction of a pre-analyte with activation light in an optical interaction region to produce analyte; and a detection light path disposed in the substrate, arranged at an oblique angle or right angle to the fluid member proximate to the photo interaction region, and that: receives the photoanalyte light from the photo interaction region; and communicates the photoanalyte light from the microchannel to a photodetector, the optical flow meter determines a flow rate of the analyte.

Abstract: A kit includes a reference probe to react with a first sample; and an analyte probe to combine with a second sample, a marker and a spectral probe to react to form the analyte probe to combine with the second sample, the marker to react with the reference probe to form the analyte probe to combine with the second sample, or a combination thereof. A process for staining includes forming a reference composition by reacting a reference probe and a first sample to form a reference; and forming an analyte composition by combining an analyte probe and a second sample to form an analyte.