Abstract: A vise assembly is disclosed. In one example, the vise assembly includes a vise body and a jaw pusher movably coupled to the vise body. The jaw pusher includes a jaw insert having a first top edge and a second top edge, where the first top edge is an outward extending radius edge. A jaw is provided having a jaw top and a jaw bottom, the jaw bottom including a recess for receiving the jaw insert, the recess including a ramped surface extending longitudinally along a top edge for receiving the jaw insert radius edge and wherein the positioned on the jaw pusher without the use of tools.

Abstract: A vise assembly is disclosed. In one example, the vise assembly includes a vise body and a jaw pusher movably coupled to the vise body. The jaw pusher includes a jaw insert having a first top edge and a second top edge, where the first top edge is an outward extending radius edge. A jaw is provided having a jaw top and a jaw bottom, the jaw bottom including a recess for receiving the jaw insert, the recess including a ramped surface extending longitudinally along a top edge for receiving the jaw insert radius edge and wherein the positioned on the jaw pusher without the use of tools.

Abstract: The engineering of a pathway converting CH4 to acetate and eventually to liquid fuels is disclosed. The engineered pathway involves an engineered reversal of the natural pathway for acetate conversion to CH4 by microbes and coupling the engineered pathway to existing and future technologies for microbial or chemical conversion of acetate to liquid fuels. In one aspect, methods for producing modified pathways and/or microbes are provided. In another aspect engineered microbes, including Methanosarcina acetivorans, that incorporate the engineered pathway are provided, which can mediate conversion of CH4 to acetate for ultimate conversion to liquid fuels. In a further aspect, specific modifications to the components of the pathway are provided.