Patents by Inventor Gregory G. Lavieu

Gregory G. Lavieu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240375097
    Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
    Type: Application
    Filed: September 13, 2023
    Publication date: November 14, 2024
    Inventors: Randall D. LOWE, JR., Alexander J. MASTROIANNI, Mark P. WHITE, Gregory G. LAVIEU, Kristin G. BEAUMONT
  • Patent number: 11801508
    Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
    Type: Grant
    Filed: January 29, 2021
    Date of Patent: October 31, 2023
    Assignee: Berkeley Lights, Inc.
    Inventors: Randall D. Lowe, Jr., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
  • Patent number: 11639495
    Abstract: Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: May 2, 2023
    Assignees: The Regents of the University of California, Berkeley Lights, Inc.
    Inventors: Alexander Marson, Gregory G. Lavieu, Annamaria Mocciaro, Theodore L. Roth, Magali Soumillon, Hayley M. Bennett
  • Publication number: 20230023831
    Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
    Type: Application
    Filed: September 8, 2022
    Publication date: January 26, 2023
    Applicant: BERKLEY LIGHTS, INC.
    Inventors: Kristin G. BEAUMONT, Peter J. BEEMILLER, Volker L.S. KURZ, Gregory G. LAVIEU, Xiaohua WANG, Aathavan KARUNAKARAN
  • Patent number: 11454629
    Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
    Type: Grant
    Filed: June 22, 2020
    Date of Patent: September 27, 2022
    Assignee: Berkeley Lights, Inc.
    Inventors: Kristin G. Beaumont, Peter J. Beemiller, Volker L. S. Kurz, Gregory G. Lavieu, Xiaohua Wang, Aathavan Karunakaran
  • Publication number: 20220250071
    Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
    Type: Application
    Filed: November 23, 2021
    Publication date: August 11, 2022
    Inventors: Troy A. LIONBERGER, Matthew E. FOWLER, Phillip J. M. ELMS, Kevin D. LOUTHERBACK, Randall D. LOWE, JR., Jian GONG, J. Tanner NEVILL, Gang F. WANG, Gregory G. LAVIEU, John A. TENNEY, Aathavan KARUNAKARAN, Anupam SINGHAL, I-Jong LIN
  • Patent number: 11273177
    Abstract: The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: March 15, 2022
    Assignee: Berkeley Lights, Inc.
    Inventors: Kevin T Chapman, Xiaohua Wang, Xiao Guan Radstrom, Yelena Bronevetsky, Guido K Stadler, Gregory G Lavieu, Annamaria Mocciaro
  • Patent number: 11203018
    Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
    Type: Grant
    Filed: October 15, 2018
    Date of Patent: December 21, 2021
    Assignee: Berkeley Lights, Inc.
    Inventors: Troy A. Lionberger, Matthew E. Fowler, Phillip J. M. Elms, Kevin D. Loutherback, Randall D. Lowe, Jr., Jian Gong, J. Tanner Nevill, Gang F. Wang, Gregory G. Lavieu, John A. Tenney, Aathavan Karunakaran, Anupam Singhal, I-Jong Lin
  • Publication number: 20210291171
    Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
    Type: Application
    Filed: January 29, 2021
    Publication date: September 23, 2021
    Applicant: Berkeley Lights, Inc.
    Inventors: Randall D. Lowe, JR., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
  • Patent number: 11103870
    Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: August 31, 2021
    Assignee: Berkeley Lights, Inc.
    Inventors: Gregory G. Lavieu, Annamaria Mocciaro, Xiao Guan Radstrom, Jason M. McEwen, Magali Soumillon, J. Tanner Nevill, Volker L. S. Kurz, Patricia A. Dyck, Ravi K. Ramenani
  • Patent number: 11007520
    Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: May 18, 2021
    Assignee: Berkeley Lights, Inc.
    Inventors: Randall D. Lowe, Jr., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
  • Publication number: 20210011015
    Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
    Type: Application
    Filed: June 22, 2020
    Publication date: January 14, 2021
    Inventors: Kristin G. Beaumont, Peter J. Beemiller, Volker L.S. Kurz, Gregory G. Lavieu, Xiaohua Wang, Aathavan Karunakaran
  • Patent number: 10705082
    Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
    Type: Grant
    Filed: December 7, 2016
    Date of Patent: July 7, 2020
    Assignee: Berkeley Lights, Inc.
    Inventors: Kristin G. Beaumont, Peter J. Beemiller, Volker L. S. Kurz, Gregory G. Lavieu, Xiaohua Wang, Aathavan Karunakaran
  • Publication number: 20200048606
    Abstract: Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.
    Type: Application
    Filed: June 27, 2019
    Publication date: February 13, 2020
    Applicants: The Regents of the University of California, Berkeley Lights, Inc.
    Inventors: Alexander Marson, Gregory G. Lavieu, Annamaria Mocciaro, Theodore L. Roth, Magali Soumillon, Hayley M. Bennett
  • Publication number: 20190275516
    Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
    Type: Application
    Filed: November 20, 2018
    Publication date: September 12, 2019
    Applicant: Berkeley Lights, Inc.
    Inventors: Randall D. Lowe, JR., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
  • Publication number: 20190240665
    Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
    Type: Application
    Filed: October 15, 2018
    Publication date: August 8, 2019
    Inventors: Troy A. LIONBERGER, Matthew E. FOWLER, Phillip J. M. ELMS, Kevin D. LOUTHERBACK, Randall D. LOWE, JR., Jian GONG, Tanner J. NEVILL, Gang F. WANG, Gregory G. LAVIEU, John A. TENNEY, Aathavan KARUNAKARAN
  • Publication number: 20190217297
    Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
    Type: Application
    Filed: January 28, 2019
    Publication date: July 18, 2019
    Applicant: Berkeley Lights, Inc.
    Inventors: Gregory G. Lavieu, Annamaria Mocciaro, Xiao Guan Radstrom, Jason M. McEwen, Magali Soumillon, J. Tanner Nevill, Volker L.S. Kurz, Patricia A. Dyck, Ravi K. Ramenani
  • Patent number: 10239058
    Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: March 26, 2019
    Assignee: Berkeley Lights, Inc.
    Inventors: Gregory G. Lavieu, Annamaria Mocciaro, Xiao Guan Radstrom, Jason M. McEwen, Magali Soumillon, J. Tanner Nevill, Volker L. S. Kurz, Patricia A. Dyck, Ravi K. Ramenani
  • Publication number: 20180147576
    Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
    Type: Application
    Filed: November 2, 2017
    Publication date: May 31, 2018
    Inventors: Gregory G. Lavieu, Annamaria Mocciaro, Xiao Guan Radstrom, Jason M. McEwen, Magali Soumillon, J. Tanner Nevill, Volker L.S. Kurz, Patricia A. Dyck, Ravi K. Ramenani
  • Publication number: 20170184583
    Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
    Type: Application
    Filed: December 7, 2016
    Publication date: June 29, 2017
    Inventors: Kristin G. Beaumont, Peter J. Beemiller, Volker L.S. Kurz, Gregory G. Lavieu, Xiaohua Wang, Aathavan Karunakaran