Abstract: An additive material is applied to a substrate, such as a paper web used as a wrapping material for cigarette manufacture. A predetermined pattern of additive material is applied to the outer surface of the wrapping material of a formed cigarette, and most preferably of a formed filtered cigarette. In particular, an application system for applying additive material of a controlled type, in a controlled manner and in a controlled location on the wrapping material of a formed two-up filtered cigarette rod is located within a tipping machine. During controlled rotation of each such formed rod (e.g., due to cooperation of a transfer drum and a laser cam), additive material is applied to the outer surface of a desired location of the wrapping material of each such rod.

Abstract: A novel isolated nucleic acid which corresponds to a gene located on human chromosome 2p21-16.3 is provided, and a polypeptide encoded thereby, together with mouse and chicken orthologs. The encoded polypeptides share a PGECCPLP motif and include an insulin-like growth factor binding domain, cysteine-rich repeats, an RGD motif and transmembrane domain, and interact with members of the transforming growth factor superfamily. The nucleic acids of the invention, and polypeptides encoded thereby, may be useful in the diagnosis and treatment of diseases including eye defects, neurodegenerative diseases, renal and kidney disease, bone and tooth abnormalities, wounds and skin damage.