Abstract: A chromatography device having a housing having an inlet and an outlet. At least two layers of media disposed between the inlet and the outlet inside of the housing forming a media stack, and wherein at least one of the layers comprises a functionalized layer. A spacer ring disposed between at the least two layers of media forming an air gap between them.

Abstract: A chromatography device having a housing having an inlet and an outlet. At least two layers of media disposed between the inlet and the outlet inside of the housing forming a media stack, with at least one of the layers comprising a functionalized layer. An optional spacer ring disposed between the two layers of media forming an air gap between them. A non-functionalized sealing layer disposed between the inlet and the outlet inside of the housing as the last layer of media in the media stack within the housing as a fluid passes from the inlet to the outlet through the media stack. A margin of the sealing layer in contact with the housing; the margin being compressed by the housing forming a compressive seal to prevent fluid from leaking to the outlet past the compressive seal.

Abstract: Described herein is a filtration media comprising: (i) a first filtration medium comprising an anion exchange nonwoven substrate, wherein the anion exchange nonwoven substrate comprises a plurality of quaternary ammonium groups; and (ii) a second filtration medium comprising a functionalized microporous membrane wherein the functionalized microporous membrane comprises a plurality of guanidyl groups; wherein the first filtration medium is positioned upstream of the second filtration medium.

Abstract: A method of purifying a biological composition includes: disposing loose cationic ligand-functionalized staple fibers and a biological composition within a mixing volume of a vessel; agitating the biological composition and the loose cationic ligand-functionalized staple fibers while they are in intimate contact with each other within the mixing volume to provide modified fibers and a purified biological composition; and separating at least a portion of the purified biological composition from the modified fibers and any loose cationic ligand-functionalized staple fibers with which it is in contact. The loose cationic ligand-functionalized staple fibers have a modified surface layer comprising a grafted acrylic polymer comprising 10 to 100 percent by weight of a cationically-ionizable monomer unit. An article for purifying a biological composition includes: a vessel having a mixing volume disposed therein; and the loose cationic ligand-functionalized staple fibers disposed within the mixing volume.

Abstract: Described herein is a filtration media comprising: (i) a first filtration medium comprising an anion exchange nonwoven substrate, wherein the anion exchange nonwoven substrate comprises a plurality of quaternary ammonium groups; and (ii) a second filtration medium comprising a functionalized microporous membrane wherein the functionalized microporous membrane comprises a plurality of guanidyl groups; wherein the first filtration medium is positioned upstream of the second filtration medium.

Abstract: A method of purifying a biological composition includes: disposing loose cationic ligand-functionalized staple fibers and a biological composition within a mixing volume of a vessel; agitating the biological composition and the loose cationic ligand-functionalized staple fibers while they are in intimate contact with each other within the mixing volume to provide modified fibers and a purified biological composition; and separating at least a portion of the purified biological composition from the modified fibers and any loose cationic ligand-functionalized staple fibers with which it is in contact. The loose cationic ligand-functionalized staple fibers have a modified surface layer comprising a grafted acrylic polymer comprising 10 to 100 percent by weight of a cationically-ionizable monomer unit. An article for purifying a biological composition includes: a vessel having a mixing volume disposed therein; and the loose cationic ligand-functionalized staple fibers disposed within the mixing volume.

Abstract: A filter frame assembly is disclosed comprising a perimeter member surrounding a fluid aperture adapted to accommodate a fluid flow from an upstream direction to a downstream direction, the perimeter member comprising a perimeter upstream surface, a perimeter downstream surface, and a perimeter outer surface connecting the perimeter upstream surface to the perimeter downstream surface. A gasket carrier is attached to the perimeter member and comprises a carrier outer portion facing generally away from the fluid aperture and a first outer portion sealing member projecting from the carrier outer portion.

Abstract: Described herein is a membrane separation module and articles and methods thereof, wherein the membrane separation module has a series of repeating layers, each layer comprising (a) a selectively permeable membrane; and (b) at least one support layer, wherein the support layer comprises a plurality of flow channels and a plurality of rails extending from the support layer, wherein the sides of two adjacent rails form a flow channel, wherein the plurality of rails on the support layer comprise a thermoplastic polymer at the distal end of at least a portion of the plurality of rails, and wherein the distal end of the plurality of rails contacts the selectively permeable membrane to form a bonded stack.

Abstract: A filter frame assembly is disclosed comprising a perimeter member surrounding a fluid aperture adapted to accommodate a fluid flow from an upstream direction to a downstream direction, the perimeter member comprising a perimeter upstream surface, a perimeter downstream surface, and a perimeter outer surface connecting the perimeter upstream surface to the perimeter downstream surface. A gasket carrier is attached to the perimeter member and comprises a carrier outer portion facing generally away from the fluid aperture and a first outer portion sealing member projecting from the carrier outer portion.

Abstract: A maskless process generates a patterned coating on a polymeric substrate, wherein the pattern is at least partially defined by the substrate topography. The process uses a high intensity/high fluence energy source to strike a coated substrate, thus selectively removing a portion of the coating. The amount and area of the coating removed is dependent on the substrate topography and the applied energy, and this forms a pattern of residual coating material on the substrate.

Abstract: An antenna adapted for placement in the window of a vehicle includes a flexible substrate having a pair of opposing surfaces, at least one of which is provided with electrically conductive metal traces in the form of a pattern configured to receive and transmit one or more electromagnetic signals. The pitch, trace width, and thickness of the metal traces are selected such that the antenna has an area-averaged resistivity of no greater than about 1 ohm/square for a pre-determined area-averaged transmission value in the visible region.