Abstract: In the field of aptamers, closed sequence solution space libraries for aptamer selection and related methods for selecting aptamers for binding to target molecules. Also, a method of treating a disease or disorder in a subject, including diagnosing, monitoring, or predicting a using at least one aptamer obtained by the closed sequence solution space libraries, and treating the diagnosed subject. Further a specific set of aptamers, which may be used for detection and/or quantification of a target molecule.

Abstract: In the field of aptamers, closed sequence solution space libraries for aptamer selection. Also, methods for selecting aptamers for binding to target molecules in which biological samples derived from individuals that differ by phenotype are contacted with the library of aptamers and the aptamer oligonucleotides that bound to the target molecules are covered. Further, methods of treating a disorder or a disease of a subject in which the disorder or disease is diagnosed using the library of aptamers and the subject is treated.

Abstract: Compositions comprising optimized aptamers capable of specifically binding to a surface protein of Clostridium difficile spore are provided. A method for detecting, enriching, separating, and/or isolating Clostridium difficile spores is provided.

Abstract: Compositions comprising optimized aptamers capable of specifically binding to a surface protein of Clostridium difficile spore are provided. A method for detecting, enriching, separating, and/or isolating Clostridium difficile spores is provided.

Abstract: The use of the change in relative frequency of aptamers in a library selected against at least one sample from at least one reference subject of known outcome, prior to and following selection of said library against a sample from a subject, as a means of diagnosing a medical state, disease or condition in said subject. Also, methods for diagnosing a medical state, disease or condition in a subject, involving correlating changes in relative frequency between aptamers to a medical state, disease or condition and diagnosing the subject based on the determined correlations.

Abstract: Disclosed is a method for the selection of aptamers that does not require immobilization of the target or the oligonucleotide library. The method includes: (i) combining a selection library of oligonucleotides that contain a random region flanked by primer recognition sites with blocker oligonucleotides that anneal to the primer recognition sites; (ii) exposing the blocked selection library to an immobilization field including random oligonucleotides and removing unbound selection library oligonucleotides; (iii) recovering the selection library oligonucleotides that bound to the immobilization field and combining with a target analyte of interest; (iv) exposing the selection library oligonucleotides-target analyte mixture to an immobilization field; and (v) recovering those selection library oligonucleotides that did not bind to the immobilization field.

Abstract: Disclosed is a method for the selection of aptamers that does not require immobilization of the target or the oligonucleotide library. The method includes: (i) combining a selection library of oligonucleotides that contain a random region flanked by primer recognition sites with blocker oligonucleotides that anneal to the primer recognition sites; (ii) exposing the blocked selection library to an immobilization field including random oligonucleotides and removing unbound selection library oligonucleotides; (iii) recovering the selection library oligonucleotides that bound to the immobilization field and combining with a target analyte of interest; (iv) exposing the selection library oligonucleotides-target analyte mixture to an immobilization field; and (v) recovering those selection library oligonucleotides that did not bind to the immobilization field.