Patents by Inventor Gregory T. Bleck
Gregory T. Bleck has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7384738Abstract: The present invention relates to the expression and screening of genomic DNA sequences encoding uncharacterized genes and proteins. The present invention provides systems utilizing unique features of retroviral replication to analyze uncharacterized genes derived from genomic DNA samples. In preferred embodiments, a segment of genomic DNA is inserted between 5? and 3? viral long terminal repeats (LTRs) in a vector (e.g., a plasmid, cosmid, or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic sequences) is then introduced into a retroviral packaging cell. The resulting provirus or proteins expression from the provirus are then analyzed.Type: GrantFiled: March 26, 2003Date of Patent: June 10, 2008Inventors: Robert D. Bremel, Gregory T. Bleck, Michael Imboden, Kurt Eakle
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Patent number: 7378273Abstract: The present invention provides novel regulatory elements and vectors for the expression of one or more proteins in a host cell. The present invention also provides methods for expressing one or more proteins, such as antibodies, in a host cell. These methods utilize the novel regulatory elements and vectors of the present invention for the expression of proteins in a host cell. The host cells are used for producing various protein products, including but not limited to pharmaceutical proteins, antibodies, variants of proteins for use in screening assays, and for direct use in high throughput screening.Type: GrantFiled: September 23, 2004Date of Patent: May 27, 2008Assignee: Gala Design, Inc.Inventor: Gregory T. Bleck
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Patent number: 7332333Abstract: The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.Type: GrantFiled: December 21, 2004Date of Patent: February 19, 2008Assignee: Gala Design, Inc.Inventors: Robert D. Bremel, Linda U. Miller, Gregory T. Bleck
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Patent number: 7169963Abstract: The present invention relates to animals that express exogenous growth factors in their milk, and in particular to pigs that express exogenous IGF-I in their milk. The present invention also relates to methods for increasing piglet weight gain and intestinal lactase activity. The present invention thus provides a method of facilitating piglet development and decreasing piglet mortality.Type: GrantFiled: September 30, 2003Date of Patent: January 30, 2007Assignee: Board of Trustees of the University of IllinoisInventors: Matthew B. Wheeler, Sharon M. Donovan, Gregory T. Bleck, Marcia Monaco-Siegel
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Patent number: 6852510Abstract: The present invention relates to the production of proteins in host cells, and, more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.Type: GrantFiled: June 29, 2001Date of Patent: February 8, 2005Assignee: Gala Design IncInventors: Robert D. Bremel, Linda U. Miller, Gregory T. Bleck
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Publication number: 20040235173Abstract: The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene and methods of making such host cells by serial transduction or transfection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.Type: ApplicationFiled: January 16, 2004Publication date: November 25, 2004Applicant: Gala Design, Inc.Inventors: Gregory T. Bleck, Robert D. Bremel, Linda U. Miller
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Publication number: 20040064841Abstract: The present invention relates to animals that express exogenous growth factors in their milk, and in particular to pigs that express exogenous IGF-I in their milk. The present invention also relates to methods for increasing piglet weight gain and intestinal lactase activity. The present invention thus provides a method of facilitating piglet development and decreasing piglet mortality.Type: ApplicationFiled: September 30, 2003Publication date: April 1, 2004Applicant: Board of Trustees of the University of IllinoisInventors: Matthew B. Wheeler, Sharon M. Donovan, Gregory T. Bleck, Marcia Monaco-Seigel
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Publication number: 20040019920Abstract: The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.Type: ApplicationFiled: March 11, 2003Publication date: January 29, 2004Applicant: Gala Design, Inc.Inventors: Robert D. Bremel, Gregory T. Bleck
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Patent number: 6677500Abstract: The present invention relates to animals that express exogenous growth factors in their milk, and in particular to pigs that express exogenous IGF-I in their milk. The present invention also relates to methods for increasing piglet weight gain and intestinal lactase activity. The present invention thus provides a method of facilitating piglet development and decreasing piglet mortality.Type: GrantFiled: August 15, 2001Date of Patent: January 13, 2004Assignee: Board of Trustees of the University of IllinoisInventors: Matthew B. Wheeler, Sharon M. Donovan, Gregory T. Bleck, Marcia Monaco-Seigel
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Publication number: 20040002062Abstract: The present invention relates to the expression and screening of genomic DNA sequences encoding uncharacterized genes and proteins. The present invention provides systems utilizing unique features of retroviral replication to analyze uncharacterized genes derived from genomic DNA samples. In preferred embodiments, a segment of genomic DNA is inserted between 5′ and 3′ viral long terminal repeats (LTRs) in a vector (e.g., a plasmid, cosmid, or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic sequences) is then introduced into a retroviral packaging cell. The resulting provirus or proteins expression from the provirus are then analyzed.Type: ApplicationFiled: March 26, 2003Publication date: January 1, 2004Applicant: Gala Design, Inc.Inventors: Robert D. Bremel, Gregory T. Bleck, Michael Imboden, Kurt Eakle
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Publication number: 20030224415Abstract: The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene. The present invention further relates to the use of integrating vectors lacking a selectable marker and growth of host cells containing such vectors in the absence of selection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.Type: ApplicationFiled: March 26, 2003Publication date: December 4, 2003Applicant: Gala Design, Inc.Inventors: Robert D. Bremel, Gregory T. Bleck, Dona York, Kurt Eakle
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Publication number: 20030092882Abstract: The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.Type: ApplicationFiled: June 29, 2001Publication date: May 15, 2003Inventors: Robert D. Bremel, Linda U. Miller, Gregory T. Bleck
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Publication number: 20020144296Abstract: The present invention relates to animals that express exogenous growth factors in their milk, and in particular to pigs that express exogenous IGF-I in their milk. The present invention also relates to methods for increasing piglet weight gain and intestinal lactase activity. The present invention thus provides a method of facilitating piglet development and decreasing piglet mortality.Type: ApplicationFiled: August 15, 2001Publication date: October 3, 2002Inventors: Matthew B. Wheeler, Sharon M. Donovan, Gregory T. Bleck, Marcia Monaco-Seigel
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Publication number: 20020106729Abstract: The present invention provides novel regulatory elements and vectors for the expression of one or more proteins in a host cell. The present invention also provides methods for expressing one or more proteins, such as antibodies, in a host cell. These methods utilize the novel regulatory elements and vectors of the present invention for the expression of proteins in a host cell. The host cells are used for producing various protein products, including but not limited to pharmaceutical proteins, antibodies, variants of proteins for use in screening assays, and for direct use in high throughput screening.Type: ApplicationFiled: June 29, 2001Publication date: August 8, 2002Inventor: Gregory T. Bleck
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Publication number: 20020104109Abstract: The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.Type: ApplicationFiled: May 4, 2001Publication date: August 1, 2002Inventors: Robert D. Bremel, Gregory T. Bleck
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Patent number: 5850000Abstract: The present invention relates to the use of a mammary specific .alpha.-lactalbumin protein to assist in the production of recombinant proteins in mammals' milk. The invention also relates to the genetically engineered mammal that produces the desired recombinant product in its milk and to the products produced by the genetically engineered mammal, including the altered composition of milk and the semen which includes the desired .alpha.-lactalbumin protein DNA sequence.Type: GrantFiled: March 22, 1996Date of Patent: December 15, 1998Assignee: Wisconsin Milk Marketing BoardInventors: Gregory T. Bleck, Robert D. Bremel
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Patent number: 5530177Abstract: The present invention relates to the use of a mammary specific .alpha.-lactalbumin regulatory sequence to assist in the production of recombinant proteins in mammals' milk. The invention also relates to the genetically engineered mammal that produces the desired recombinant product in its milk and to the products produced by the genetically engineered mammal, including the altered composition of milk and the semen which includes the desired .alpha.-lactalbumin protein DNA sequence.Type: GrantFiled: June 4, 1993Date of Patent: June 25, 1996Assignee: Wisconsin Milk Marketing BoardInventors: Gregory T. Bleck, Robert D. Bremel