Patents by Inventor Gregory W. deHart

Gregory W. deHart has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Activated Formylglycine-Generating Enzymes and Methods of Producing and Using the Same
    Publication number: 20230357814
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Application
    Filed: April 7, 2023
    Publication date: November 9, 2023
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Activated formylglycine-generating enzymes and methods of producing and using the same
    Patent number: 11655492
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Grant
    Filed: June 2, 2021
    Date of Patent: May 23, 2023
    Assignee: R.P. Scherer Technologies, LLC
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Anti-Her2 Antibody-Maytansine Conjugates and Methods of Use Thereof
    Publication number: 20220023440
    Abstract: The present disclosure provides anti-HER2 antibody-maytansine conjugate structures. The disclosure also encompasses methods of production of such conjugates, as well as methods of using the same.
    Type: Application
    Filed: July 22, 2021
    Publication date: January 27, 2022
    Inventors: David Rabuka, Aaron Edward Albers, Robyn M. Barfield, Gregory W. deHart, Penelope M. Drake, Romas Alvydas Kudirka, Albert W. Garofalo, Jesse M. McFarland
  • Activated Formylglycine-Generating Enzymes and Methods of Producing and Using the Same
    Publication number: 20220002775
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Application
    Filed: June 2, 2021
    Publication date: January 6, 2022
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Anti-HER2 antibody-maytansine conjugates and methods of use thereof
    Patent number: 11097013
    Abstract: The present disclosure provides anti-HER2 antibody-maytansine conjugate structures. The disclosure also encompasses methods of production of such conjugates, as well as methods of using the same.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: August 24, 2021
    Assignee: Redwood Bioscience, Inc.
    Inventors: David Rabuka, Aaron Edward Albers, Robyn M. Barfield, Gregory W. deHart, Penelope M. Drake, Romas Alvydas Kudirka, Albert W. Garofalo, Jesse M. McFarland
  • Activated formylglycine-generating enzymes and methods of producing and using the same
    Patent number: 11053529
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Grant
    Filed: May 4, 2020
    Date of Patent: July 6, 2021
    Assignee: R.P. Scherer Technologies, LLC
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Activated Formylglycine-Generating Enzymes and Methods of Producing and Using the Same
    Publication number: 20200370084
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Application
    Filed: May 4, 2020
    Publication date: November 26, 2020
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Activated formylglycine-generating enzymes and methods of producing and using the same
    Patent number: 10683527
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: June 16, 2020
    Assignee: R.P. Scherer Technologies, LLC
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Activated Formylglycine-Generating Enzymes and Methods of Producing and Using the Same
    Publication number: 20190390240
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Application
    Filed: May 16, 2019
    Publication date: December 26, 2019
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Activated formylglycine-generating enzymes and methods of producing and using the same
    Patent number: 10344311
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Grant
    Filed: January 4, 2018
    Date of Patent: July 9, 2019
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • ANTI-HER2 ANTIBODY-MAYTANSINE CONJUGATES AND METHODS OF USE THEREOF
    Publication number: 20190111151
    Abstract: The present disclosure provides anti-HER2 antibody-maytansine conjugate structures. The disclosure also encompasses methods of production of such conjugates, as well as methods of using the same.
    Type: Application
    Filed: September 26, 2018
    Publication date: April 18, 2019
    Inventors: David Rabuka, Aaron Edward Albers, Robyn M. Barfield, Gregory W. deHart, Penelope M. Drake, Romas Alvydas Kudirka, Albert W. Garofalo, Jesse M. McFarland
  • Aldehyde-tagged immunoglobulin polypeptides and methods of use thereof
    Patent number: 10183998
    Abstract: The present disclosure provides aldehyde-tagged immunoglobulin (Ig) polypeptides that can be converted by a formylglycine-generating enzyme to produce a 2-formylglycine (FGly)-modified Ig polypeptide. An FGly-modified Ig polypeptide can be covalently and site-specifically bound to a moiety of interest to provide an Ig conjugate. The disclosure also encompasses methods of production of such aldehyde-tagged Ig polypeptides, FGly-modified Ig polypeptides, and Ig conjugates, as well as methods of use of same.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: January 22, 2019
    Assignee: Redwood Bioscience, Inc.
    Inventors: Robyn M. Barfield, Mark Alan Breidenbach, Gregory W. deHart, David Rabuka
  • Anti-HER2 antibody-maytansine conjugates and methods of use thereof
    Patent number: 10124070
    Abstract: The present disclosure provides anti-HER2 antibody-maytansine conjugate structures. The disclosure also encompasses methods of production of such conjugates, as well as methods of using the same.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: November 13, 2018
    Assignee: Redwood Bioscience, Inc.
    Inventors: David Rabuka, Aaron Edward Albers, Robyn M. Barfield, Gregory W. deHart, Penelope M. Drake, Romas Alvydas Kudirka, Albert W. Garofalo, Jesse M. McFarland
  • Activated Formylglycine-Generating Enzymes and Methods of Producing and Using the Same
    Publication number: 20180223322
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Application
    Filed: January 4, 2018
    Publication date: August 9, 2018
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Activated formylglycine-generating enzymes and methods of producing and using the same
    Patent number: 9951367
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: April 24, 2018
    Assignee: R.P. Scherer Technologies, LLC
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Aldehyde-Tagged Immunoglobulin Polypeptides and Methods of Use Thereof
    Publication number: 20170166639
    Abstract: The present disclosure provides aldehyde-tagged immunoglobulin (Ig) polypeptides that can be converted by a formylglycine-generating enzyme to produce a 2-formylglycine (FGly)-modified Ig polypeptide. An FGly-modified Ig polypeptide can be covalently and site-specifically bound to a moiety of interest to provide an Ig conjugate. The disclosure also encompasses methods of production of such aldehyde-tagged Ig polypeptides, FGly-modified Ig polypeptides, and Ig conjugates, as well as methods of use of same.
    Type: Application
    Filed: November 28, 2016
    Publication date: June 15, 2017
    Inventors: Robyn M. Barfield, Mark Alan Breidenbach, Gregory W. deHart, David Rabuka
  • Aldehyde-tagged immunoglobulin polypeptides and methods of use thereof
    Patent number: 9540438
    Abstract: The present disclosure provides aldehyde-tagged immunoglobulin (Ig) polypeptides that can be converted by a formylglycine-generating enzyme to produce a 2-formylglycine (FGly)-modified Ig polypeptide. An FGly-modified Ig polypeptide can be covalently and site-specifically bound to a moiety of interest to provide an Ig conjugate. The disclosure also encompasses methods of production of such aldehyde-tagged Ig polypeptides, FGly-modified Ig polypeptides, and Ig conjugates, as well as methods of use of same.
    Type: Grant
    Filed: January 13, 2012
    Date of Patent: January 10, 2017
    Assignee: Redwood Bioscience, Inc.
    Inventors: Robyn M. Barfield, Mark Alan Breidenbach, Gregory W. deHart, David Rabuka
  • Activated Formylglycine-Generating Enzymes and Methods of Producing and Using the Same
    Publication number: 20160230205
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Application
    Filed: December 18, 2015
    Publication date: August 11, 2016
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Anti-Her2 Antibody-Maytansine Conjugates and Methods of Use Thereof
    Publication number: 20150352225
    Abstract: The present disclosure provides anti-HER2 antibody-maytansine conjugate structures. The disclosure also encompasses methods of production of such conjugates, as well as methods of using the same.
    Type: Application
    Filed: May 27, 2015
    Publication date: December 10, 2015
    Inventors: David Rabuka, Aaron Edward Albers, Robyn M. Barfield, Gregory W. deHart, Penelope M. Drake, Romas Alvydas Kudirka, Albert W. Garofalo, Jesse M. McFarland
  • Aldehyde-Tagged Immunoglobulin Polypeptides and Methods of Use Thereof
    Publication number: 20120183566
    Abstract: The present disclosure provides aldehyde-tagged immunoglobulin (Ig) polypeptides that can be converted by a formylglycine-generating enzyme to produce a 2-formylglycine (FGly)-modified Ig polypeptide. An FGly-modified Ig polypeptide can be covalently and site-specifically bound to a moiety of interest to provide an Ig conjugate. The disclosure also encompasses methods of production of such aldehyde-tagged Ig polypeptides, FGly-modified Ig polypeptides, and Ig conjugates, as well as methods of use of same.
    Type: Application
    Filed: January 13, 2012
    Publication date: July 19, 2012
    Inventors: Robyn M. Barfield, Mark Alan Breidenbach, Gregory W. deHart, David Rabuka