Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.

Abstract: In one example, a centrifugal fan includes a bearing, a shaft rotatably supported by the bearing, fan blades operatively connected to and rotatable with the shaft to take in air axially and to exhaust air radially, and a groove in the shaft extending axially along an outer surface of the shaft through the bearing.

Abstract: Systems and methods for continuous production of at least one biological molecule of interest are provided. More specifically, the systems include (i) at least one culture bioreactor; (ii) at least one hydrocyclone; and (iii) at least one production bioreactor; wherein the culture bioreactor and the production bioreactor are linked by the hydrocyclone. The methods include the steps of (i) culturing a plurality of host cells capable of producing a biologic molecule of interest in at least one culture bioreactor; (ii) inoculating at least one production bioreactor with cells obtained from step (i); and (iii) culturing the cells in the production bioreactor.

Abstract: The invention relates to methods of improving protein production, e.g., large-scale commercial protein production, e.g., antibody production, utilizing a modified fed-batch cell culture method comprising a cell growth phase and a polypeptide production phase. The modified fed-batch cell culture method combines both cell culture perfusion and fed-batch methods to achieve higher titers of polypeptide products. Because the modified fed-batch cell culture method of the invention produces higher polypeptide product titers than fed-batch culture alone, it will substantially improve commercial-scale protein production. The invention also relates to a perfusion bioreactor apparatus comprising a fresh medium reservoir connected to a bioreactor by a feed pump, a recirculation loop connected to the bioreactor, wherein the recirculation loop comprises a filtration device, e.g., ultrafiltration or microfiltration, and a permeate pump connecting the filtration device to a permeate collection container.

Abstract: A method for increasing the force required to separate a solidified deformable material into at least two portions includes adding unmodified fibers and/or modified fibers having high tensile strength, high modulus of elasticity and high shock resistance to the deformable material; the modified fibers having surfaces with integral protuberances and/or attached silica particles emanating from each surface. The modified fibers, when mixed with the deformable material, ultimately form a solidified matrix having increased tensile strength (when compared to the solidified material without having the modified fibers) and increased resistance to separating into two or more portions when a force impacts or strikes a portion of the solidified deformable material.

Abstract: A method for assembling a shell section of a wind turbine blade includes providing a support, the support comprising a surface having a primary surface portion configured for supporting a first component and a secondary surface portion configured for supporting a second component. The method also includes arranging a second component on the secondary surface portion such that an outer surface of the second component is facing the secondary surface portion. The method further includes arranging a first component on the primary surface portion such that an outer surface of the first component is facing the primary surface portion, and such that a first primary joint surface of the first component is facing a second joint surface of the second component. Arranging the first component includes applying a force to the first component to force the outer surface of the first component towards the primary surface portion and the first primary joint surface towards the second joint surface.

Abstract: A stencil device for forming configurations in deformable material includes a first member having ridge portions integrally joined to channel portions that promote the insertion of the ridge portions of the first member into deformable material; and a second member having ridge portions integrally joined to protrusion portions that promote the insertion of the ridge portions of the second member into deformable material; whereby, the first and second members ultimately form a design pattern across a preselected surface of deformable material by alternating the insertion and removal of said first and second members across the preselected surface of the deformable material.

Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.

Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

Abstract: A reusable pool mold for forming a swimming pool coping or overhang above a peripheral portion of pool water in accordance with the present invention includes multiple bracket members detachably secured to a liner receiver for a swimming pool. Each bracket having a lower portion of an outer vertical member integrally joined to an outer portion of a horizontal member, and having an inner portion of said horizontal member integrally joined to an upper portion of an inner vertical member. Each bracket member includes a securing member for detachably securing a bracket member to the liner receiver. The pool mold includes a form member that continuously extends above a water portion of a swimming pool; the form member receiving deformable material that ultimately solidifies, whereupon, the form member and brackets are removed from the solidified material to reveal a configured coping or overhang continuously extending above a peripheral portion of water inside the swimming pool.

Abstract: A reusable countertop mold in accordance with the present invention includes a bracket member detachably secured to a rail portion of a cabinet member for a substantially horizontal substrate that receives deformable material upon a surface of the substrate. The bracket member includes vertical, horizontal and channel members integrally joined together for enabling a channel member to removably receive the rail member. The mold further includes a locking bolt for detachably securing the bracket member to the rail portion of the support member.

Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

Abstract: A reusable countertop mold in accordance with the present invention includes a bracket member detachably secured to a rail portion of a cabinet member for a substantially horizontal substrate that receives deformable material upon a surface of the substrate. The bracket member includes vertical, horizontal and channel members integrally joined together for enabling a channel member to removably receive the rail member. The mold further includes a locking bolt for detachably securing the bracket member to the rail portion of the support member.

Abstract: The invention describes a core shell microcapsule wherein the capsule shell is hydrolysable.

Abstract: A stencil device for forming configurations in deformable material includes a first member having ridge portions integrally joined to channel portions that promote the insertion of the ridge portions of the first member into deformable material; and a second member having ridge portions integrally joined to protrusion portions that promote the insertion of the ridge portions of the second member into deformable material; whereby, the first and second members ultimately form a design pattern across a preselected surface of deformable material by alternating the insertion and removal of said first and second members across the preselected surface of the deformable material.

Abstract: A reusable countertop mold in accordance with the present invention includes a bracket member detachably secured to a rail portion of a cabinet member for a substantially horizontal substrate that receives deformable material upon a surface of the substrate. The bracket member includes vertical, horizontal and channel members integrally joined together for enabling a channel member to removably receive the rail member. The mold further includes a locking bolt for detachably securing the bracket member to the rail portion of the support member.

Abstract: An apparatus and method for securing a deformable material to a wall structure such that the deformable material is allowed to solidify to form a predetermined configuration upon the wall structure, includes securing a fabric member to a wall structure, the fabric member having a configuration that supports a deformable material that ultimately solidifies; a plurality of offset strips (when required) that separate the fabric member from the wall structure to provide an air gap; and fasteners that maintain the relative position of the fabric upon the wall structure throughout the application of the deformable material upon the fabric member, the configuring of a design into the deformable material, and after the deformable material has solidified.

Abstract: A reusable pool mold for forming a swimming pool coping or overhang above a peripheral portion of pool water in accordance with the present invention includes multiple bracket members detachably secured to a liner receiver for a swimming pool. Each bracket having a lower portion of an outer vertical member integrally joined to an outer portion of a horizontal member, and having an inner portion of said horizontal member integrally joined to an upper portion of an inner vertical member. Each bracket member includes a securing member for detachably securing a bracket member to the liner receiver. The pool mold includes a form member that continuously extends above a water portion of a swimming pool; the form member receiving deformable material that ultimately solidifies, whereupon, the form member and brackets are removed from the solidified material to reveal a configured coping or overhang continuously extending above a peripheral portion of water inside the swimming pool.

Abstract: A method for assembling a shell section of a wind turbine blade includes providing a support, the support comprising a surface having a primary surface portion configured for supporting a first component and a secondary surface portion configured for supporting a second component. The method also includes arranging a second component on the secondary surface portion such that an outer surface of the second component is facing the secondary surface portion. The method further includes arranging a first component on the primary surface portion such that an outer surface of the first component is facing the primary surface portion, and such that a first primary joint surface of the first component is facing a second joint surface of the second component. Arranging the first component includes applying a force to the first component to force the outer surface of the first component towards the primary surface portion and the first primary joint surface towards the second joint surface.

Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N?1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N?1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.