Abstract: The disclosed computer-implemented method may include systems for generating personalized avatar reactions during live video broadcasts. For example, the systems and methods described herein can access a social networking system user's profile to identify an avatar associated with the social networking system user. The systems and methods can generate an avatar reaction by modifying one or more features of the avatar based on a corresponding emoticon reaction. Once generated, the social networking system user can select the avatar reaction for addition to an ephemeral reaction stream associated with a live video broadcast. Various other methods, systems, and computer-readable media are also disclosed.

Abstract: Botulinum neurotoxin proteins and fragments thereof that bind and/or cleave a noncanonical substrate, e.g., non-neuronal SNARE proteins such as human SNAP-23 (hSNAP-23) and SNAP-29 (hSNAP-29), are described.

Abstract: There are provided, inter alia, methods for reacting an enzyme and its substrate, methods for purifying a protein and an enzyme reactor and its use thereof.

Abstract: Disclosed herein, inter alia, are compositions and methods useful for improving detection of analytes. The compositions and methods provided include polymer-wrapped viral particles useful, inter alia, for the detection of PSMA.

Abstract: A method of improving protein functionality includes obtaining a protein such as protein contained within exclusion bodies contained within bacteria or yeast used for the recombinant expression of the protein. The exclusion bodies are solubilized using a chaotropic agent such as guanidine or urea. The protein is then subject to denaturation conditions and optionally purified. A liquid containing the denatured protein is loaded into a vessel that is angled relative to horizontal. The vessel is then rotated in the angled configuration at a rate within that is less than 10,000 RPM for period of time. Refolded protein is formed by the high shear conditions formed in the thin film of fluid formed in the inner surface of the rotating vessel. Refolding can be performed in a batch mode or a continuous mode. The process may be scaled up for industrial applications by using multiple vessels.

Abstract: Provided herein are, inter alia, biosensors and electrochemical cells comprising electronically conductive polymers and viral particles; diagnostic kits; and methods of detecting compounds in samples.

Abstract: The present disclosure provides a method for sequencing nucleic acids. The method can include polymerase catalyzed incorporation of nucleotides into a nascent nucleic acid strand against a nucleic acid template, wherein the polymerase is attached to a charge sensor that detects nucleotide incorporation events. One or more non-natural nucleotide types that each produce a unique signatures at the charge sensor can be used to uniquely identify different nucleotides in the template nucleic acid.

Abstract: There are provided, inter alia, methods for reacting an enzyme and its substrate, methods for purifying a protein and an enzyme reactor and its use thereof.

Abstract: The present disclosure provides a method for sequencing nucleic acids. The method can include polymerase catalyzed incorporation of nucleotides into a nascent nucleic acid strand against a nucleic acid template, wherein the polymerase is attached to a charge sensor that detects nucleotide incorporation events. One or more non-natural nucleotide types that each produce a unique signatures at the charge sensor can be used to uniquely identify different nucleotides in the template nucleic acid.

Abstract: A light control unit that includes a housing sized and shaped as a standard electrical dimmer switch that can reside in an a standard electrical Handy Box and at least one integrated circuit that comprises a brightness dimming control adapted to reduce and increase brightness of one or more light sources, for example a series of light sources, electronically connected to the at least one integrated circuit and a correlated color temperature (CCT) control adapted to change color temperature of light emitted from the one or more light sources.

Abstract: The invention provides methods and compositions for detecting a change in a nucleic acid polymerase conformation involving contacting a nucleic acid polymerase non-covalently attached to a single walled carbon nanotube (SWNT) with a first nucleotide or first nucleotide analog and a template and detecting the conformationally changed nucleic acid polymerase by measuring a first electrical conductance change in the SWNT between the nucleic acid polymerase and the conformationally changed nucleic acid polymerase. The method is useful for sequencing of polynucleotides.

Abstract: A light control unit that includes a housing sized and shaped as a standard electrical dimmer switch that can reside in an a standard electrical Handy Box and at least one integrated circuit that comprises a brightness dimming control adapted to reduce and increase brightness of one or more light sources, for example a series of light sources, electronically connected to the at least one integrated circuit and a correlated color temperature (CCT) control adapted to change color temperature of light emitted from the one or more light sources.

Abstract: There are provided, inter alia, viral compositions including a viral particle in contact with a polymer and the polymer is linked to a recognition moiety, and methods of use thereof.

Abstract: Disclosed herein, inter alia, are compositions and methods useful for improving detection of analytes. The compositions and methods provided include polymer-wrapped viral particles useful, inter alia, for the detection of PSMA.

Abstract: A method of improving protein functionality includes obtaining a protein such as protein contained within exclusion bodies contained within bacteria or yeast used for the recombinant expression of the protein. The exclusion bodies are solubilized using a chaotropic agent such as guanidine or urea. The protein is then subject to denaturation conditions and optionally purified. A liquid containing the denatured protein is loaded into a vessel that is angled relative to horizontal. The vessel is then rotated in the angled configuration at a rate within that is less than 10,000 RPM for period of time. Refolded protein is formed by the high shear conditions formed in the thin film of fluid formed in the inner surface of the rotating vessel. Refolding can be performed in a batch mode or a continuous mode. The process may be scaled up for industrial applications by using multiple vessels.

Abstract: A biosensor that is capable of detecting the presence and/or concentration of an analyte or biomarker includes at least one electrically conductive electrode operatively coupled to an impedance analyzer for measuring the change in the resistive impedance ?ZRe of the electrode in response to an applied alternating current at a plurality of frequencies. In one embodiment, at least one electrode is covered with a self-assembled monolayer that is chemically bonded to a surface. A plurality of virus particles such as phage viruses are immobilized on the self-assembled monolayer and may be exposed to a test or sample solution. The virus particles may be obtained from phage-displayed libraries to detect a wide variety of targets including, for example, DNA, RNA, small molecules, and proteins or polypeptides. In another embodiment, the virus particles are electrostatically bound to a substrate in between a pair of elongated electrodes disposed on a substrate.

Abstract: A single molecule sensing device includes a first electrode, a second electrode and a single-walled carbon nanotube (SWNT) connected to the first and second electrodes. At least one linker molecule having first and second functional groups is functionalized with a sidewall of the SWNT, the at least one linker molecule having the first functional group non-covalently functionalized with a sidewall of the single-walled carbon nanotube. A single sensitizing molecule having at least one functional group is functionalized with the second functional group of the at least one linker molecule.

Abstract: Grafting M13 bacteriophage into an array of poly(3,4-ethylenedioxythiophene) (PEDOT) nanowires generated hybrids of conducting polymers and replicable genetic packages (rgps) such as viruses. The incorporation of rgps into the polymeric backbone of PEDOT occurs during electropolymerization via lithographically patterned nanowire electrodeposition (LPNE). The resultant arrays of rgps-PEDOT nanowires enable real-time, reagent-free electrochemical biosensing of analytes in physiologically relevant buffers.

Abstract: PSMA ligands, compositions, and methods therefore are disclosed where the ligand is a peptide having the sequence X1X2CVEVX3QNSCX4X5 where X1-X5 are independently a natural or non-natural amino acid or a peptide having the sequence CALCEFLG [SEQ ID NO: 1]. Especially preferred aspects include diagnostic reagents for detection and/or quantification of PSMA in a sample, therapeutic reagents, and diagnostic imaging reagents.

Abstract: The transformation yield of electroporation is increased by using higher DNA concentrations and DNA affinity purification. Fusion proteins of a viral coat protein variant and a heterologous polypeptide are useful in phage display systems.