Abstract: Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from rearranged V-gene sequences of unimmunised mammal.

Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.

Abstract: Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from rearranged V-gene sequences of unimmunised mammal.

Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.

Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.

Abstract: Apparatuses and methods for dispensing magnetic stripe cards, smart cards, other cards, and/or other items from kiosks and other structures are disclosed herein. In one embodiment, a card dispensing apparatus includes at least a first card hopper and a card transport assembly. The first card hopper is configured to hold a stack of cards that includes at least a first card stacked on a second card. The card transport assembly includes a card carrier moveable between a first position proximate to the first card hopper and a second position spaced apart from the first card hopper. The card carrier is configured to lift the first card off the second card when the card carrier is in the first position. The card carrier is further configured to release the first card toward an outlet when the card carrier is in the second position.

Abstract: The invention provides a method for selecting, from a repertoire of polypeptides, a population of functional polypeptides which bind a target ligand in a first binding site and a generic ligand in a second binding site, which generic ligand is capable of binding functional members of the repertoire regardless of target ligand specificity, comprising the steps of: a) contacting the repertoire with the generic ligand and selecting functional polypeptides bound thereto; and b) contacting the selected functional polypeptides with the target ligand and selecting a population of polypeptides which bind to the target ligand. The invention accordingly provides a method by which a polypeptide repertoire is preselected, according to functionality as determined by the ability to bind the generic ligand, and the subset of polypeptides obtained as a result of such preselection is then employed for further selection according to the ability to bind the target ligand.

Abstract: Mobile resource management systems including a location module configured to identify at least one of a plurality of potential customers having a location within a proximity zone. A mobile agent device is associated with the mobile agent and is configured to transmit a location signal indicative of a location of the mobile agent device. A mobile agent location module is configured to receive the signal and to determine the location of the mobile agent device as a function of the received signal. A customer communication module is configured to transmit a notification to the at least one potential customer as a function of the location of the mobile agent device.

Abstract: A method of notifying a potential customer of a mobile agent being proximate to the location of the potential customer including defining a route to be traveled by the mobile agent, defining a proximity zone for the mobile agent as a function of the route, and identifying a potential customer having a location within the proximity zone. The method also includes determining a location of the mobile agent and notifying the potential customer as a function of the determined location of the mobile agent.

Abstract: The present invention relates to a method for the generation of immunoglobulin molecules of predetermined specificity. In particular, the invention relates to a method for the retargeting of the epitope binding specificity of one or more antibodies using single variable domains which exhibit a dominant epitope binding specificity.

Abstract: A mobile resource management system and method for managing the arrival of a mobile agent at a location of a customer, the system including a mobile agent location module, a customer communication module, and a mobile agent communication module. The mobile agent location module is configured for identifying a location of a mobile agent and the customer communication module configured for transmitting a notification to a customer of an availability of the mobile agent for arrival of the mobile agent at a location of the customer as a function, at least in part, of the identified mobile agent location, prompting the customer to confirm the arrival of the mobile agent at the customer location, and receiving a confirmation from the customer in response to the prompting. The mobile agent communication module is configured for transmitting schedule and customer appointment information to the mobile agent in response to receiving the customer confirmation.

Abstract: A customer appointment management system and method with mobile resource management including a customer communication module configured for transmitting to a customer a notification of a pending arrival of a mobile agent at the customer location for an appointment and receiving a confirmation from the customer confirming the appointment. The system also includes a mobile agent communication module configured for transmitting a schedule and customer appointment information to the mobile agent in response to receiving the customer confirmation.

Abstract: The present invention relates to a simple method for generating antibody-based structures suitable for in vivo use. In particular, the invention relates to a method for the generation of antibody-based structures suitable for in vivo use comprising the steps of: (a) selecting an antibody single variable domain having an epitope binding specificity; and (b) attaching the single domain of step (a) to an effector group. Uses of molecules generated using the method of the Invention are also described.

Abstract: Methods and systems for detecting coin fraud in coin-counting machines and other devices that count and/or sort coins and other objects. In one embodiment, the method includes discriminating multiple coins to determine a number of real coins and a number of faux coins. In one aspect of this embodiment, the faux coins can have one or more coin characteristics falling generally close to corresponding characteristics of the real coins. The method can further include determining a quotient based on the number of real coins and faux coins. If the determined quotient is greater than or equal to a selected threshold value, then the transaction can be identified as being possibly fraudulent. In the event of a possibly fraudulent transaction, the method can include controlling the transaction, for example, by returning any uncounted coins to a user, or by halting the transaction.

Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.

Abstract: The invention provides a method for selecting, from a repertoire of polypeptides, a population of functional polypeptides which bind a target ligand in a first binding site and a generic ligand in a second binding site, which generic ligand is capable of binding functional members of the repertoire regardless of target ligand specificity, comprising the steps of: a) contacting the repertoire with the generic ligand and selecting functional polypeptides bound thereto; and b) contacting the selected functional polypeptides with the target ligand and selecting a population of polypeptides which bind to the target ligand. The invention accordingly provides a method by which a polypeptide repertoire is preselected, according to functionality as determined by the ability to bind the generic ligand, and the subset of polypeptides obtained as a result of such preselection is then employed for further selection according to the ability to bind the target ligand.

Abstract: Apparatuses and methods for dispensing cards and other items. In one embodiment, a card dispensing apparatus includes at least first and second card hoppers and a movable card carriage. The card carriage is movable between a first position proximate to the first card hopper and a second position proximate to the second card hopper. The card carriage is configured to receive a first card from the first card hopper when in the first position and a second card from the second card hopper when in the second position. In one aspect of this embodiment, the card carriage can dispense at least the first card into a card outlet chute when the first card has been sufficiently read by a card reader. Alternatively, the card carriage can dispense the first card into a card escrow chute when the first card has not been sufficiently read by the card reader.

Abstract: The present invention comprises a platform on which a ball machine is temporarily mounted and can be secured. Beneath the platform a type of pivot or other variety of mobility providing device(s) is/are attached, to allow the platform to move about in multiple directions and/or rotate. The support platform can be allowed to roll, rotate, tilt and turn while a ball machine is attached to it. Handle(s) can be attached to the platform to allow someone to manually manage/dictate the aim or position of the apparatus, thus control or change the direction of each ball fed when a ball machine is in operation and attached to the structure.

Abstract: Methods and systems for detecting coin fraud in coin-counting machines and other devices that count and/or sort coins and other objects. In one embodiment, the method includes discriminating multiple coins to determine a number of real coins and a number of faux coins. In one aspect of this embodiment, the faux coins can have one or more coin characteristics falling generally close to corresponding characteristics of the real coins. The method can further include determining a quotient based on the number of real coins and faux coins. If the determined quotient is greater than or equal to a selected threshold value, then the transaction can be identified as being possibly fraudulent. In the event of a possibly fraudulent transaction, the method can include controlling the transaction, for example, by returning any uncounted coins to a user, or by halting the transaction.

Abstract: A process control method for controlling a polyolefin polymerization process, such as a solution polyethylene process, which includes five steps. The first step is to flow a stream of a solvent into a polyolefin reactor, such as flowing a stream of heptane solvent into the reactor at essentially a constant rate. The second step is to add a principal monomer to the stream of solvent, such as by adding ethylene at a controllable rate to the stream of solvent. The third step is to add a co-monomer to the stream of solvent, such as by adding 1-octene at a controllable rate to the stream of solvent.