Abstract: Exemplary embodiments are directed to providing a method of preparing an anatomical vessel contained within a tissue bundle for ligation, including positioning a cannula adjacent to the tissue bundle, wherein the positioning is non-penetrating, the cannula including a first extendable member, a second extendable member, a Doppler wand, and a distal end, transmitting ultrasound signals toward the vessel through the tissue bundle with the Doppler wand, receiving ultrasound signals reflected by the vessel through the tissue bundle with the Doppler wand, invaginating the tissue bundle with the first extendable member on a first side of the tissue bundle, invaginating the tissue bundle with the second extendable member on a second side of the bundle opposite the first side, and interpenetrating a length of ligation material between the first and second extendable members on a side of the vessel opposite the cannula distal end.

Abstract: A low profile parenchymal bolt having a cap, a body, and a base with two rods located through the cap that the base. Levers are located at the cap and are interconnected with the rods. Moving the levers outwardly pulls the rods and base towards the cap compressing the body and causing its outer diameter to increase into contact with an opening in the skull of a patient to form a fluid-tight seal and fixing the bolt in position. A lumen permits mounting an instrument through the bolt and when the body is compressed, the lumen reduces in size thus clamping the instrument in place in the bolt and forming a fluid-tight seal. Through use of non-ferric materials, artifacts in MRI are reduced.