Patents by Inventor Guan-Chiun Lee
Guan-Chiun Lee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11970691Abstract: Provided is an immobilized thermostable trehalose synthase including an amino acid sequence of a trehalose synthase domain and an amino acid sequence of a cellulose binding domain. Also provided is a method for converting maltose into trehalose or for converting sucrose into trehalulose by using the immobilized thermostable trehalose synthase.Type: GrantFiled: March 19, 2021Date of Patent: April 30, 2024Assignee: National Taiwan Normal UniversityInventor: Guan-Chiun Lee
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Patent number: 11685939Abstract: A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil-water mixture is provided, wherein the oil-water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil-water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55° C. to 65° C. and an oil phase layer is extracted. (vi) The cooling and filtering step is performed to obtain cis-unsaturated fatty acid.Type: GrantFiled: April 14, 2021Date of Patent: June 27, 2023Assignees: CHANT OIL CO., LTD., National Taiwan Normal UniversityInventors: Guan-Chiun Lee, Ting-Chun Kuo, Shun-Niang Chen, Chia-Chi Lin
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Publication number: 20220186204Abstract: Provided is an immobilized thermostable trehalose synthase including an amino acid sequence of a trehalose synthase domain and an amino acid sequence of a cellulose binding domain. Also provided is a method for converting maltose into trehalose or for converting sucrose into trehalulose by using the immobilized thermostable trehalose synthase.Type: ApplicationFiled: March 19, 2021Publication date: June 16, 2022Applicant: National Taiwan Normal UniversityInventor: Guan-Chiun LEE
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Publication number: 20220098628Abstract: A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil-water mixture is provided, wherein the oil-water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil-water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55° C. to 65° C. and an oil phase layer is extracted. (vi) The cooling and filtering step is performed to obtain cis-unsaturated fatty acid.Type: ApplicationFiled: April 14, 2021Publication date: March 31, 2022Inventors: Guan-Chiun LEE, Ting-Chun KUO, Shun-Niang CHEN, Chia-Chi LIN
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Patent number: 9970035Abstract: A method for producing biodiesel is provided, which includes providing a recombinant Candida rugosa lipase; reacting the recombinant C. rugosa lipase and a non-edible oil; and isolating the biodiesel from the reacted solution.Type: GrantFiled: April 21, 2016Date of Patent: May 15, 2018Assignee: National Taiwan Normal UniversityInventors: Guan-Chiun Lee, Ting-Chun Kuo
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Publication number: 20170202792Abstract: A method for treating a tau-associated disease is disclosed, which comprises the step of administering a pharmaceutical composition to a subject in need. Particularly, a method for treating Alzheimer's disease is disclosed, which comprises the step of administering a pharmaceutical composition to a subject in need.Type: ApplicationFiled: August 16, 2016Publication date: July 20, 2017Inventors: Hsiu-Mei HSIEH, Ying-Chieh SUN, Guan-Chiun LEE, Guey-Jen LEE-CHEN, Ming-Tsan SU, Hui-Chen HUANG, Yu-Shao HSIEH, Chia-Jen HSU
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Publication number: 20170137850Abstract: A method for producing biodiesel is provided, which includes providing a recombinant Candida rugosa lipase; reacting the recombinant C. rugosa lipase and a non-edible oil; and isolating the biodiesel from the reacted solution.Type: ApplicationFiled: April 21, 2016Publication date: May 18, 2017Inventors: Guan-Chiun LEE, Ting-Chun KUO
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Patent number: 9629835Abstract: A method for treating tau-associated disease by administering a pharmaceutical composition comprising a quinoline derivative to a subject in need is disclosed. Particularly, a method for treating Alzheimer's disease by administering a pharmaceutical composition comprising a quinoline derivative to a subject in need is disclosed. The two disclosed quinoline derivatives have the inhibition effect for GSK-3? kinase activity, so as the two disclosed quinoline derivatives have the ability for inhibiting tau hyperphosphorylation or reducing tau aggregation.Type: GrantFiled: August 16, 2016Date of Patent: April 25, 2017Assignee: National Taiwan Normal UniversityInventors: Guey-Jen Lee-Chen, Hsiu-Mei Hsieh, Guan-Chiun Lee, Ying-Chieh Sun
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Patent number: 9463197Abstract: A method for treating an abnormal polyglutamine-mediated disease is disclosed, which comprises: administering a pharmaceutical composition comprising a trehalose-based compound to a subject in need. Additionally, the pharmaceutical composition optionally further comprises a trehalase inhibitor.Type: GrantFiled: July 15, 2014Date of Patent: October 11, 2016Assignee: NATIONAL TAIWAN NORMAL UNIVERSITYInventors: Guey-Jen Lee-Chen, Hsiu-Mei Hsieh, Guan-Chiun Lee
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Publication number: 20150025028Abstract: A method for treating an abnormal polyglutamine-mediated disease is disclosed, which comprises: administering a pharmaceutical composition comprising a trehalose-based compound to a subject in need. Additionally, the pharmaceutical composition optionally further comprises a trehalase inhibitor.Type: ApplicationFiled: July 15, 2014Publication date: January 22, 2015Inventors: Guey-Jen LEE-CHEN, Hsiu-Mei HSIEH, Guan-Chiun LEE
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Patent number: 7981639Abstract: A method for producing a monosaccharide-rich syrup from starch-containing produce. The method includes treating a starch-containing produce slurry with a first starch hydrolyzing enzyme that hydrolyzes starch to oligosaccharide and a second starch hydrolyzing enzyme that hydrolyzes starch or oligosaccharide to glucose. The starch-containing produce can be further treated with an enzyme that converts glucose to other monosaccharides, or treated with a microorganism that converts glucose to a fermentation product. Also within the scope of this invention is a method for producing a syrup rich in a disaccharide, such as trehalose.Type: GrantFiled: February 19, 2004Date of Patent: July 19, 2011Assignee: Academia SinicaInventors: Jei-Fu Shaw, Guan-Chiun Lee, Jen-Jye Chen
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Patent number: 7927850Abstract: The present invention relates to the isolation and functional identification of a novel acid and heat resistant trehalose synthase enzyme cloned from Picrophilus torridus. More particularly, the present invention discloses the DNA sequence for the Picrophilus torridus trehalose synthase gene, PTTS, which when expressed in a heterologous host such as Escherichia coli, provides enzymatic activity that catalyzes the direct interconversion of maltose and trehalose through intramolecular transglycosylation. Additionally, the present invention teaches methods of use of PTTS for production of trehalose as well as for production of various useful compounds comprising trehalose.Type: GrantFiled: July 21, 2006Date of Patent: April 19, 2011Assignee: Academia SinicaInventors: Jei-Fu Shaw, Guan-Chiun Lee, Yi-Shan Chen
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Patent number: 7655453Abstract: The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.Type: GrantFiled: August 20, 2007Date of Patent: February 2, 2010Assignee: Academia SinicaInventors: Jei-fu Shaw, Guan-chiun Lee, Shye-Jye Tang
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Publication number: 20090053795Abstract: The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.Type: ApplicationFiled: August 20, 2007Publication date: February 26, 2009Applicant: Academia SinicaInventors: Jei-Fu Shaw, Guan-chiun Lee, Shye-Jye Tang
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Patent number: 7468429Abstract: The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.Type: GrantFiled: February 7, 2006Date of Patent: December 23, 2008Assignee: Academia SinicaInventors: Jei-Fu Shaw, Guan-Chiun Lee, Shye-Jye Tang
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Publication number: 20080182311Abstract: The present invention relates to the isolation and functional identification of a novel acid and heat resistant trehalose synthase enzyme cloned from Picrophilus torridus. More particularly, the present invention discloses the DNA sequence for the Picrophilus torridus trehalose synthase gene, PTTS, which when expressed in a heterologous host such as Escherichia coli, provides enzymatic activity that catalyzes the direct interconversion of maltose and trehalose through intramolecular transglycosylation. Additionally, the present invention teaches methods of use of PTTS for production of trehalose as well as for production of various useful compounds comprising trehalose.Type: ApplicationFiled: July 21, 2006Publication date: July 31, 2008Inventors: Jei-Fu Shaw, Guan-Chiun Lee, Yi-Shan Chen
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Publication number: 20060121528Abstract: The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.Type: ApplicationFiled: February 7, 2006Publication date: June 8, 2006Inventors: Jei-Fu Shaw, Guan-Chiun Lee, Shye-Jye Tang
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Patent number: 7052879Abstract: The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.Type: GrantFiled: August 31, 2001Date of Patent: May 30, 2006Assignee: Academia SinicaInventors: Jei-Fu Shaw, Guan-Chiun Lee, Shye-Jye Tang
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Publication number: 20040161829Abstract: A method for producing a monosaccharide-rich syrup from starch-containing produce. The method includes treating a starch-containing produce slurry with a first starch hydrolyzing enzyme that hydrolyzes starch to oligosaccharide and a second starch hydrolyzing enzyme that hydrolyzes starch or oligosaccharide to glucose. The starch-containing produce can be further treated with an enzyme that converts glucose to other monosaccharides, or treated with a microorganism that converts glucose to a fermentation product. Also within the scope of this invention is a method for producing a syrup rich in a disaccharide, such as trehalose.Type: ApplicationFiled: February 19, 2004Publication date: August 19, 2004Inventors: Jei-Fu Shaw, Guan-Chiun Lee, Jen-Jye Chen
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Publication number: 20030124701Abstract: The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.Type: ApplicationFiled: August 31, 2001Publication date: July 3, 2003Inventors: Jei-Fu Shaw, Guan-Chiun Lee, Shye-Jye Tang