Abstract: A protein microarray, a use thereof, and a detection method thereof are provided. The protein microarray includes a carrier with a protein array block on a surface thereof and at least two kinds of proteins immobilized on the protein array block. The at least two kinds of proteins include an extracellular region or a receptor binding region of the spike protein of a virus or a variant thereof. The protein microarray can detect the protective efficacy of vaccines, antibody drugs, or small molecule drugs against viral infection, or detect the immune response of an individual after vaccination or infection with a variant strain of the virus. The detection method of protein microarray includes the steps of adding the serum, a first fluorescent labeled human angiotensin converting enzyme 2, and a second fluorescent labeled anti-human immunoglobulin antibody to the protein microarray array and detecting optical signals.

Abstract: A protein microarray, a detection method thereof, and an evaluation method thereof are provided. The protein microarray includes a carrier including a protein array block on a surface thereof and at least one protein immobilized on the protein array block. The at least one protein includes a spike protein and a nucleocapsid protein, and can bind to a first antibody in a to-be-tested sample. A method for detecting virus infection or evaluating the ability of a bioagent to block virus infection includes the steps of respectively adding blood, serum, plasma or the bioagent and a second antibody to the protein microarray and detecting an optical signal. The protein microarray can be used to detect a coronavirus and an influenza virus, and achieve the effect of quickly, sensitively, and accurately confirming whether a subject is infected by the virus.