Abstract: A method for screening a key stratum for overburden separation grouting in a coal mine includes: selecting overall analysis objects based on coal mine planning; conducting a primary screening of the analysis objects based on a scope of a subsidence zone; conducting a secondary screening of the analysis objects based on whether there is an old goaf; determining key stratums for screened-in analysis objects, prioritizing the key stratums based on a number of separation spaces and a range of a separation height, setting a safe height based on a diversion fissure zone, and prioritizing numbers of groutable layers based on a potential separation space and a water resistance of a rock stratum; prioritizing the groutable layers based on a fault impact; and comprehensively analyzing grouting priority levels of the key stratums based on results of the three prioritizations, and grouting separation layers of the key stratums in order of priority.