Abstract: A multi-channel capillary electrophoresis microchip for analysis of multiple samples comprising: at least two sample reservoirs, at least two sample channels corresponding to said sample reservoirs, a sample buffer reservoir, a sample waste reservoir, a separation buffer reservoir, a separation waste reservoir, a sample loading channel, and a separation channel, wherein each of said sample channels is connected at one end to said sample loading channel at a place between said sample buffer reservoir and said intersection of said sample loading channel and said separation channel, and the other end of each of said sample channels is connected to each of said sample reservoirs. A method of capillary electrophoresis separation for sequentially analysis of multiple samples.