Abstract: A dynamic loose vehicle formation method based on real-time online navigation map, which belongs to the field of vehicle formation technologies. The method includes a driver inputs travel information of a destination and waypoints in a GIS app; the GIS app uploads the travel information to a GIS server; the driver chooses to join a recommended formation or create a formation in the GIS app; when the driver chooses the formation and joins the formation, the formation information is displayed on the GIS app interface and sent to other vehicles within the formation for information interaction; when the driver chooses to leave the formation and leaves the formation, the formation is adjusted and the GIS server updates the formation information.

Abstract: The present invention uses microfluidics droplets for studying the growth dynamics of communities of bacteria in the presence of various perturbations such as various chemicals or other microorganisms and to establish microbial assemblies model which is representative of a microbiota of interest and to examine their growth dynamics after perturbations such as with an array of chemicals or other microorganisms. In particular, the present invention refers to a method and apparatus for assessing the effect of a chemical or biological substance on the growth of microorganisms.

Abstract: In a first aspect, the present invention relates to a method of producing a library of microorganisms, the method comprising the steps of: a. providing a first fluid comprising at least one single cell, b. dispersing said first fluid comprising at least one single cell in a second fluid, thereby obtaining a plurality of single-layer microfluidic droplets, wherein at least one single- layer microfluidic droplet comprises at least one single cell, wherein the second fluid is immiscible with the first fluid, c. optionally, adding to said at least one single-layer microfluidic droplet a third fluid comprising a sensing compound, wherein the third fluid is miscible with said first fluid, and wherein the third fluid is immiscible with said second fluid, d. injecting said at least one single-layer microfluidic droplet optionally comprising the sensing compound into a fourth fluid, wherein said fourth fluid is immiscible with said second fluid, thereby obtaining at least one double-layer microfluidic droplet, e.

Abstract: The invention relates to a method for the identification of a first microorganism potentially secreting an effector compound, said first microorganism thereby having either i. an inhibitory effect on the cell division activity of a second microorganism or ii. an enhancing effect on the cell division activity of a second microorganism, the method comprising: a. providing a cell from a first microorganism which potentially produces an effector compound of interest and a cell from a second detector microorganism; b. introducing both cells into a microdroplet for incubation; c. introducing the microdroplet into a microfluidic system; d. analyzing in said microfluidic system the cell of the second microorganism for the exhibition of an enhanced growth effect or the exhibition of an inhibited growth effect stemming from said effector compound. The invention also relates to a microorganism or effector compound identified by the method according to the invention.

Abstract: The invention relates to a microfluidic system in which the droplets flow through an off-chip delay line in a linear sequential order. This ensures that all the droplets are incubated for the same amount of time and under the same conditions as they flow through the delay line. The invention further relates to the use of this microfluidic system for high throughput screening methods or in vitro evolution methods.

Abstract: The invention relates to a method for the identification of a first microorganism potentially secreting an effector compound, said first microorganism thereby having either i. an inhibitory effect on the cell division activity of a second microorganism or ii. an enhancing effect on the cell division activity of a second microorganism, the method comprising: a. providing a cell from a first microorganism which potentially produces an effector compound of interest and a cell from a second detector microorganism; b. introducing both cells into a microdroplet for incubation; c. introducing the microdroplet into a microfluidic system; d. analyzing in said microfluidic system the cell of the second microorganism for the exhibition of an enhanced growth effect or the exhibition of an inhibited growth effect stemming from said effector compound. The invention also relates to a microorganism or effector compound identified by the method according to the invention.

Abstract: A method for the analysis of microorganisms, which produce a compound, the method comprising: a. providing a microorganism which produces a compound of interest and a detector microorganism which comprises a reporter gene or reporter gene operon, wherein the microorganism producing said compound of interest and the detector microorganism are combined into single droplets, wherein each droplet comprises at least one cell of each strain; b. subjecting the droplets to a microfluidic system; c. analyzing the droplets for the activation of the reporter gene of the detector strain; d. sorting and collecting the droplets comprising the detector microorganism with expressed reporter gene.