Abstract: The present disclosure provides a colloidal gold immunochromatographic joint inspection card for diquat and paraquat in the field of on-site rapid detection, as well as a preparation method and application. The joint inspection card includes a PVC base plate, where the PVC base plate is sequentially provided with a sample pad, a gold-labelled pad, a nitrocellulose membrane and a water-absorbing pad along the chromatographic direction; the gold-labelled pad is adsorbed with an antibody solution labeled with paraquat colloidal gold and an antibody solution labeled with diquat colloidal gold; the nitrocellulose membrane is provided with a T1 detection line, a T2 detection line and a quality control line along the chromatographic direction; and an area of the T1 detection line is coated with paraquat complete antigen, an area of the T2 detection line is coated with diquat complete antigen, and the quality control line is coated with goat anti-mouse IgG.