Patents by Inventor Gudrun Schmitz-Agheguian
Gudrun Schmitz-Agheguian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8008054Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: GrantFiled: June 3, 2010Date of Patent: August 30, 2011Assignee: Roche Molecular Systems, Inc.Inventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Publication number: 20110020898Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: ApplicationFiled: June 3, 2010Publication date: January 27, 2011Applicant: Roche Diagnostics GmBHInventors: Waltraud ANKENBAUER, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Patent number: 7759107Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: GrantFiled: June 9, 2008Date of Patent: July 20, 2010Assignee: Roche Diagnostics GmbHInventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Publication number: 20090093043Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: ApplicationFiled: June 9, 2008Publication date: April 9, 2009Applicant: Roche Diagnostics GmBHInventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Patent number: 7425423Abstract: A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: GrantFiled: October 1, 1997Date of Patent: September 16, 2008Assignee: Roche Diagnostics GmbHInventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Publication number: 20060099596Abstract: The present invention is directed to a method for identification of a pathogenic organism from a predetermined group of pathogens, comprising (i) isolating a clinical sample containing at least partially purified nucleic acid, (ii) subjecting at least a first aliquot of said clinical specimen to at least one amplification and detection reaction in one reaction vessel comprising (iia) an amplification step using at least a first set of amplification primers capable of amplifying a preselected nucleic acid sequence region from several or all members of said predetermined group of pathogens, (iib) a detection step using at least 2, 3 or multiple hybridization reagents, said reagents together being capable of specifically detecting a pre-selected nucleic acid sequence region from all members of said group of pathogens, said detection step (iib) comprising steps monitoring hybridization of each of said hybridization reagents at a pre-selected temperature, said hybridization being indicative for at least the genusType: ApplicationFiled: December 2, 2003Publication date: May 11, 2006Applicant: Roche Molecular Systems, Inc.Inventors: Gerd Haberhausen, Thomas Emrich, Gregor Sagner, Martin Moczko, Gudrun Schmitz-Agheguian
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Publication number: 20050019789Abstract: A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophilum. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.Type: ApplicationFiled: February 2, 2004Publication date: January 27, 2005Applicant: Roche Diagnostics GMBHInventors: Waltraud Ankenbauer, Gudrun Schmitz-Agheguian, Elizaveta Bonch-Osmolovskaya, Vitaly Svetlichny, Ursula Markau, Bernhard Angerer, Astrid Reiser
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Patent number: 6692932Abstract: A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophiluml. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.Type: GrantFiled: June 10, 1999Date of Patent: February 17, 2004Inventors: Waltraud Ankenbauer, Gudrun Schmitz-Agheguian, Elizaveta Bonch-Osmolovskaya, Vitaly Svetlichny, Ursula Markau, Bernhard Angerer, Astrid Reiser
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Patent number: 6468775Abstract: A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3′-5′ exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.Type: GrantFiled: November 22, 1999Date of Patent: October 22, 2002Assignee: Roche Molecular Systems Inc.Inventors: Waltraud Ankenbauer, Ursula Markau, Vitaly Svetlichny, Gudrun Schmitz-Agheguian, Astrid Reiser, Bernhard Angerer, Christine Ebenbichler, Frank Laue, Elizaveta Bonch-Osmolovskaya
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Patent number: 5134069Abstract: The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Streptomyces. It can be used to recognize and cleave the double-stranded DNA sequence ##STR2## and its complementary sequence.Type: GrantFiled: November 16, 1990Date of Patent: July 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Bruno Frey, Gudrun Schmitz-Agheguian, Michael Jarsch, Christoph Kessler
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Patent number: 5134067Abstract: The type II restriction endonuclease RleAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Rhizobium. It can be used to recognize and cleave the double-stranded DNA sequence CCCACA(N).sub.12/9 and its complementary sequence.Type: GrantFiled: November 16, 1990Date of Patent: July 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Michael Jarsch, Gudrun Schmitz-Agheguian, Christoph Kessler
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Patent number: 5134068Abstract: Type II restriction endonuclease McrI is disclosed. This endonuclease has recognition sequence: ##STR1## wherein R is G or A and Y is C or T, and the cleavage site indicated by the arrows. The endonuclease is preferably obtained from organism of genus Micrococcus. It can be used to recognize and cleave double stranded DNA sequence:5'-CGRYCG-3'and its complementary sequence.Type: GrantFiled: November 16, 1990Date of Patent: July 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Hans J. Hoeltke, Michael Jarsch, Gudrun Schmitz-Agheguian, Christoph Kessler