Abstract: The invention relates to a cooling apparatus, especially for cryogenically preserving biological samples, including a duct (5) for delivering a coolant (3) to a cooling chamber (1), a heater (6) that has an adjustable first heating performance (P2) for heating the coolant (3) delivered to the cooling chamber (1), a first temperature sensor (8-10) for measuring the temperature (T2-T4) in the cooling chamber (1), a second temperature sensor (7) for measuring the temperature (Ti) of the coolant (3) delivered to the cooling chamber (I), and a regulator (11) for regulating the temperature. The regulator (11) is embodied as a multiple regulator which detects several temperatures (T1-T4) as control variables and/or adjusts several heating performances (P1, P2) as manipulated variables. The invention further relates to a corresponding operating method.

Abstract: The invention relates to a cooling apparatus, especially for cryogenically preserving biological samples, including a duct (5) for delivering a coolant (3) to a cooling chamber (1), a heater (6) that has an adjustable first heating performance (P2) for heating the coolant (3) delivered to the cooling chamber (1), a first temperature sensor (8-10) for measuring the temperature (T2-T4) in the cooling chamber (1), a second temperature sensor (7) for measuring the temperature (Ti) of the coolant (3) delivered to the cooling chamber (I), and a regulator (11) for regulating the temperature. The regulator (11) is embodied as a multiple regulator which detects several temperatures (T1-T4) as control variables and/or adjusts several heating performances (P1, P2) as manipulated variables. The invention further relates to a corresponding operating method.

Abstract: A method for forming a three-dimensional cell arrangement (1) of biological cells is disclosed, including the steps of preparation of the cell arrangement (1) on a flexible substrate (10) and deformation of the substrate (10), wherein the deformation of the substrate (10) is brought about by an attractive force exerted by the cells (2) on the substrate (10). A substrate (10), is also disclosed, made from a flexible material and including a substrate surface (11) for adhesion of a cell arrangement (1) of biological cells, wherein the substrate surface (11) has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate (10) and the substrate (10) has a flexibility such that the substrate (10) is deformable with the action of the attraction force.

Abstract: A conditioning device (100) for biological cells includes a support (10), adapted to support at least one biological cell (1) in a supporting zone (11), and a contact device (20), adapted to support at least one conditioning sample (3) and to displace the at least one conditioning sample (3) relative to the support (10) in such a manner that the at least one conditioning sample (3) touches the at least one biological cell (1), the contact device (20) including at least two mechanical supporting elements (21, 22) that can be displaced from various spatial directions towards the support (10). The invention also relates to methods for conditioning, especially imprinting or differentiating biological cells and to uses of the conditioning device.

Abstract: A conditioning device (100) for biological cells includes a support (10), adapted to support at least one biological cell (1) in a supporting zone (11), and a contact device (20), adapted to support at least one conditioning sample (3) and to displace the at least one conditioning sample (3) relative to the support (10) in such a manner that the at least one conditioning sample (3) touches the at least one biological cell (1), the contact device (20) including at least two mechanical supporting elements (21, 22) that can be displaced from various spatial directions towards the support (10). The invention also relates to methods for conditioning, especially imprinting or differentiating biological cells and to uses of the conditioning device.

Abstract: A method for forming a three-dimensional cell arrangement (1) of biological cells is disclosed, including the steps of preparation of the cell arrangement (1) on a flexible substrate (10) and deformation of the substrate (10), wherein the deformation of the substrate (10) is brought about by an attractive force exerted by the cells (2) on the substrate (10). A substrate (10), is also disclosed, made from a flexible material and including a substrate surface (11) for adhesion of a cell arrangement (1) of biological cells, wherein the substrate surface (11) has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate (10) and the substrate (10) has a flexibility such that the substrate (10) is deformable with the action of the attraction force.

Abstract: A method and devices for controlling access to a data processing device, in particular a data memory, having an access filter circuit (10) are described, whereby the access filter circuit (10) is operated with enabled access in a read-write mode at a normal operating temperature and with limited access at an access protection temperature which is lower than the normal operating temperature.

Abstract: In the method proposed, microparticles suspended in a liquid or droplets suspended in a liquid with which they are immiscible are shaped by high-frequency electric fields in a three-dimensional electrode array of a size in the micrometer or submicrometer range and subsequently consolidated by prior art chemical bonding procedures or by physical methods. The disposition, geometry and control of the electrodes determine the shape of the particles. The particles themselves must have a conductivity and/or relative dielectric constant lower than the solution surrounding them. For some, this can be achieved only at certain frequencies in the kHz and MHz band which are determined by the passive electrical properties of the particles and the surrounding solution. The particles or droplets are repelled by the electrodes so that they are shaped in the free solution without making contact with any surface and can then be consolidated.