Abstract: The present application relates to the field of cell immunity, and discloses an interacting protein screening platform for preparing a cell membrane. A cell membrane protein and a membrane protein encoding gene to be tested are respectively expressed in different cells; after staining, cells expressing different membrane proteins and cells expressing target proteins are co-incubated; and then interacting membrane proteins are obtained by screening, and specific sequences thereof are determined by sequencing. The screening platform provided in the present application can screen a variety of cell membrane proteins and membrane protein-based engineering cells, and has strong specificity, thereby greatly improving the efficiency of screening interacting proteins and reducing the difficulty, providing a simple and convenient tool for studying the mechanism of action of cell membrane proteins, and improving the screening throughput.

Abstract: Disclosed herein are trogocytosis based TCR ligand discovery platforms and methods of using the same, trogocytosis based TCR discovery platforms and methods of using the same, and trogocytosis based ligand discovery platforms and methods of using the same. Also disclosed herein are isolated cells, nucleotides, sequences, and sequence databases produced using trogocytosis based discovery platforms.