Abstract: The present invention relates to a culture medium for disperse mycobacteria comprising at least one detergent and an antifoam agent, whereby said antifoam agent is a member of the silicone family. In addition, the invention provides for methods for the activation of dipressed mycobacteria, for obtaining a standardized mycobyterial culture or for the production of (a), purified protein derivates(s) (PDD) which comprise the cultivation of mycobacteria in the active medium disclosed herein. Furthermore, the use of standardized mycobacterial cultures obtained by the method of the invention for the preparation of pharmaceutical or diagnostic compositions is described.

Abstract: The present invention relates to a recombinant Vibrio cholerae strain, a vaccine comprising said strain, the use of said strain, and methods for its production. The recombinant strain according to the invention is comprising DNA sequences coding for at least the ETEC pili CFA/1, CS3 and CS6, wherein the DNA sequences are operationally linked to one or more promoters. The strain of the invention may be used in a vaccine composition for immunization against ETEC and/or cholera. The present invention further relates to methods for enhancing the surface expression of ETEC pili in a V. cholerae strain.

Abstract: The present invention relates to a culture medium for disperse mycobacteria comprising at least one detergent and an antifoam agent, whereby said antifoam agent is a member of the silicone family. In addition, the invention provides for methods for the activation of dipressed mycobacteria, for obtaining a standardized mycobyterial culture or for the production of (a), purified protein derivates(s) (PDD) which comprise the cultivation of mycobacteria in the active medium disclosed herein. Furthermore, the use of standardized mycobacterial cultures obtained by the method of the invention for the preparation of pharmaceutical or diagnostic compositions is described.