Abstract: Various methods for visualization of output from a liquid-liquid chromatographic instrument are provided. One or more analytes detected by a liquid-liquid chromatographic instrument are visualized by providing a data set comprising a plurality of data points corresponding to one or more analytes detected by the instrument, wherein the data points comprise at least one parameter related to a K-value or a parameter from which a K-value can be determined. A K-value is calculated for at least a portion of the data set, and at least a portion of those K-values transformed by a reciprocal transformation to generate output data having a transformed K-value, wherein the transformed K-value is a real number for all K undergoing the transformation, thereby ensuring that all analytes detected by the instrument are plotted in a single chromatogram. The output data is provided to a user.

Abstract: Application of a reference mixture of natural products for systematic analysis and comparison of the properties of biphasic solvent systems in counter-current/partition chromatography. Because the reference mixture is comprised of compounds with varying polarities, functional groups, and structural features it provides a rational method for mapping the optimal resolution polarity range of a particular solvent system. The mapping of optimal resolution polarity ranges of solvent systems provided for the description of the overall optimal resolution polarity range of a solvent system family, comprised of the same solvents in different proportions. By comparing the reference mixture performance in the individual members of a solvent system family, the solvent system that best functions as the representative of, or portal to, the solvent system families is determined. Use of the reference mixture also afforded a method to compare the overall optimal resolution polarity ranges of solvent system families.

Abstract: Provided herein are various methods and systems for analyzing natural products by quantitative proton nuclear magnetic resonance (qHNMR). A method is provided for quantitative and qualitative determination of a natural product by 1HNMR and decoupling 13C nuclei from the protons in the sample containing the natural product. The resultant spectrum wherein the decoupling provides a cleaner spectrum is used to provide both structural and quantitative information about species within the sample. In an aspect, the decoupling is provided by globally optimized alternating-phase rectangular pulses (GARP). The methods presented herein are optionally used to detect impurities in a reference material and verify the purity level of a reference material.

Abstract: Various methods for visualization of output from a liquid-liquid chromatographic instrument are provided. One or more analytes detected by a liquid-liquid chromatographic instrument are visualized by providing a data set comprising a plurality of data points corresponding to one or more analytes detected by the instrument, wherein the data points comprise at least one parameter related to a K-value or a parameter from which a K-value can be determined. A K-value is calculated for at least a portion of the data set, and at least a portion of those K-values transformed by a reciprocal transformation to generate output data having a transformed K-value, wherein the transformed K-value is a real number for all K undergoing the transformation, thereby ensuring that all analytes detected by the instrument are plotted in a single chromatogram. The output data is provided to a user.

Abstract: Application of a reference mixture of natural products for systematic analysis and comparison of the properties of biphasic solvent systems in counter-current/partition chromatography. Because the reference mixture is comprised of compounds with varying polarities, functional groups, and structural features it provides a rational method for mapping the optimal resolution polarity range of a particular solvent system. The mapping of optimal resolution polarity ranges of solvent systems provided for the description of the overall optimal resolution polarity range of a solvent system family, comprised of the same solvents in different proportions. By comparing the reference mixture performance in the individual members of a solvent system family, the solvent system that best functions as the representative of, or portal to, the solvent system families is determined. Use of the reference mixture also afforded a method to compare the overall optimal resolution polarity ranges of solvent system families.