Abstract: A computer based biological sequence analysis method provides, after a training phase adopting data from screening experiments, either an evaluation of an input sequence expressing the performance with reference to the chemical-physical feature object of the screening experiment, or at least an optimized output sequence. The method provides the use of a set or library of sequences derived from DMS experiments and SELEX for the generation of a second set of high efficiency biological sequences, whereby high efficiency means, for example, high catalysis capacity, high fitness, high ability to bind to a specific target, high fluorescence activity and, in general, a high performance with reference to the chemical-physical properties of a molecule which are defined at the start and can be selected through experiments.