Abstract: An encapsulated probiotic, an encapsulating layer and a method of encapsulating probiotics are disclosed. The encapsulated probiotic includes an encapsulating layer and probiotics, and the encapsulating layer includes a nano-film having two layers for encapsulating probiotics. A first layer is formed through biological macromolecules and metal ions on surfaces of the probiotics by covalent cross-linking or metal chelating action in situ, and a second layer is formed by interactions between a bio-enzyme and the biological macromolecules. The nano-film formed in situ on cell walls of probiotics is used for encapsulation and protection of probiotics.

Abstract: A biomass-based encapsulating material for protection of probiotic activity and an encapsulating method are disclosed. The biomass-based encapsulating material includes a nano-film having two layers for encapsulating probiotics. A first layer is formed through natural biological macromolecules and metal ions on surfaces of the probiotics by covalent cross-linking or metal chelating action in situ, and a second layer is formed by interactions between a bio-enzyme and the natural biological macromolecules. The nano-film formed in situ on cell walls of probiotics is used for encapsulation and protection of probiotics. The nano-film is made of natural biomass, has low cost and has no toxic side effects. The relative activity of encapsulated probiotics is close to or higher than 90%, whether they are cultivated in environments under high concentration of antibiotics or active-free radicals. The relative activity of unprotected probiotics is below 30%, or even completely unable to survive.