Patents by Inventor Guilhem Faure

Guilhem Faure has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250171811
    Abstract: Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of CRISPR systems and transposable elements. CRISPR-associated transposons (CASTs) are bacterial RNA-guided Tn7-like transposons that can insert large DNA cargoes at targeted loci in bacteria. To adapt CAST for use on the human genome, Applicants screened 30 CAST I-B systems for activity on extrachromosomal DNA in human cells. Of the six active orthologs identified, Applicants engineered a I-B2 system from Tolypothrix sp. PCC7910 (TolCAST) to achieve RNA-guided DNA insertion in the genome of living human cells.
    Type: Application
    Filed: February 3, 2025
    Publication date: May 29, 2025
    Inventors: Feng Zhang, Makoto Saito, Guilhem Faure
  • Publication number: 20250120915
    Abstract: Described in certain example embodiments herein are engineered delivery vesicle generations systems capable of producing engineered delivery vesicles containing two or more different retroelement polypeptides. Also described herein are methods of making and using the engineered delivery vesicles, such as to deliver one or more cargoes.
    Type: Application
    Filed: December 23, 2024
    Publication date: April 17, 2025
    Inventors: Guilhem Faure, Feng Zhang, Blake Lash, Rumya Raghavan, Victoria Madigan
  • Publication number: 20250064976
    Abstract: Described herein are engineered paraneoplastic Ma protein (PNMA) capable of forming a capsid. In some embodiments, the engineered PNMA proteins comprise one or more modifications that enhance binding or loading of a cargo into the capsid, one or more modifications that modify cell-specificity of the capsid, one or more modifications that enhance intracellular delivery of the capsid, or a combination thereof. Also described herein are delivery systems comprising capsids comprising an engineered PNMA protein and a cargo.
    Type: Application
    Filed: November 15, 2024
    Publication date: February 27, 2025
    Inventors: Feng Zhang, Victoria Madigan, Yugang Zhang, Rumya Raghavan, Max Wilkinson, Guilhem Faure, Rhiannon Macrae
  • Publication number: 20240409920
    Abstract: Recent advances in the understanding of two CRISPR-Cas systems, namely, Type VI (Cas13a-d) and Type III (Type III-A-B), have shown both of them to have the ability to efficiently target RNA. Provided herein are compositions and methods for trans-splicing precursor mRNA using a catalytically-inactive Cas protein (dCas) and a trans-splicing construct comprising a guide RNA, an intron, a splice acceptor, donor RNA, and a poly A tail. The dCas is capable of forming a complex with the guide RNA and directing sequence-specific binding of the complex to a target precursor mRNA for genetic modification and any polypeptides derived thereof. The technology has important potential therapeutic applications such as correcting genetic mutations through exon replacement, insertion of transgenes, and increasing gene expression, and in non-therapeutic applications such as cell- and tissue-specific diagnostics.
    Type: Application
    Filed: October 14, 2022
    Publication date: December 12, 2024
    Inventors: Feng Zhang, Michael Segel, Guilhem Faure, Catherine Liu
  • Publication number: 20240110203
    Abstract: The present application provides systems, methods and compositions used for targeted gene modification, targeted insertion, perturbation of gene transcripts, nucleic acid editing. Novel nucleic acid targeting systems comprise components of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and transposable elements. Specifically, the disclosure provides an engineered composition comprising: a programmable DNA-binding protein and two or more Tn7-like transposition proteins, wherein at least one of the Tn7-like transposition proteins is connected to the DNA-binding protein or otherwise capable of forming a complex with the DNA-binding protein, wherein the DNA-binding protein comprising a Cas protein including a Cas12k protein, and wherein two or more Tn7-like transposition proteins consisting of TnsB, TnsC, and TniQ.
    Type: Application
    Filed: January 7, 2022
    Publication date: April 4, 2024
    Inventors: Feng Zhang, Jonathan Strecker, Guilhem Faure
  • Patent number: 11851702
    Abstract: Systems and methods for rapid diagnostics related to the use of isothermal amplification reagents for detection of microbial species, including coronavirus, and methods of use, are provided.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: December 26, 2023
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure
  • Publication number: 20230265420
    Abstract: The present application provides systems, methods and compositions used for targeted gene modification, targeted insertion, perturbation of gene transcripts, nucleic acid editing. Novel nucleic acid targeting systems comprise components of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and transposable elements.
    Type: Application
    Filed: June 18, 2021
    Publication date: August 24, 2023
    Inventors: Feng Zhang, Jonathan Strecker, Alim Ladha, Guilhem Faure
  • Patent number: 11639523
    Abstract: Engineered or non-naturally occurring systems and compositions comprising a novel Cas12b and a guide molecule. Also provided include methods of use the systems and compositions, including in treating and diagnosing diseases.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: May 2, 2023
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure
  • Publication number: 20230127948
    Abstract: Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences for detection of coronavirus, including multiplex lateral flow diagnostic devices and methods of use, are provided.
    Type: Application
    Filed: February 12, 2021
    Publication date: April 27, 2023
    Inventors: Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure, Feng Zhang
  • Publication number: 20230091690
    Abstract: Described herein are guided excision-transposition systems, methods of making, and uses thereof.
    Type: Application
    Filed: December 30, 2020
    Publication date: March 23, 2023
    Applicants: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Feng Zhang, Guilhem Faure, Daniel Strebinger, Makoto Saito
  • Publication number: 20220380758
    Abstract: Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of CRISPR systems and transposable elements.
    Type: Application
    Filed: October 30, 2020
    Publication date: December 1, 2022
    Applicants: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Makoto Saito, Feng Zhang, Guilhem Faure
  • Publication number: 20220325334
    Abstract: Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences for detection of coronavirus, including multiplex lateral flow diagnostic devices and methods of use, are provided.
    Type: Application
    Filed: April 27, 2022
    Publication date: October 13, 2022
    Applicants: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure
  • Patent number: 11453907
    Abstract: Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences for detection of coronavirus, including multiplex lateral flow diagnostic devices and methods of use, are provided.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: September 27, 2022
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure
  • Publication number: 20210292823
    Abstract: Systems and methods for rapid diagnostics related to the use of isothermal amplification reagents for detection of microbial species, including coronavirus, and methods of use, are provided.
    Type: Application
    Filed: June 5, 2020
    Publication date: September 23, 2021
    Inventors: Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure
  • Publication number: 20210292824
    Abstract: Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences for detection of coronavirus, including multiplex lateral flow diagnostic devices and methods of use, are provided.
    Type: Application
    Filed: June 5, 2020
    Publication date: September 23, 2021
    Inventors: Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure
  • Publication number: 20210292721
    Abstract: Engineered or non-naturally occurring systems and compositions comprising a novel Cas12b and a guide molecule. Also provided include methods of use the systems and compositions, including in treating and diagnosing diseases.
    Type: Application
    Filed: June 5, 2020
    Publication date: September 23, 2021
    Inventors: Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure