Abstract: Provided is a method of analyzing interactions between nucleic acid segments in a nucleic acid complex. Specifically, restriction enzymes that recognize four-base site are used for digestion, followed by a two-step ligation method. The overall process is simple and easy to control, realizing the efficient and sensitive detection of nucleic acid interaction segments.

Abstract: A method for preparing a high-performance tantalum target, a high-performance target prepared by the method, and a use of the high-performance target. The method for preparing the high-performance tantalum target comprises: firstly, preparing a tantalum ingot into a forging blank by a method of cold forging in conjunction with hot forging; then, rolling the forging blank by a hot rolling method; and finally, performing leveling, and performing discharging, milling and surface treatment according to a size of a finished product, so as to obtain the tantalum target. The tantalum target prepared by the method has uniform crystallization, with a grain size between 50 ?m and 120 ?m. A texture component where a texture (110) dominants in the thickness direction of the target is obtained. A total proportion of three textures (111), (110) and (100) is between 40% and 50%, ensuring a consistent sputtering rate of the tantalum target during use.