Abstract: The present disclosure relates to a method for detecting TCEP content in ADC by LC-MS/MS. The ADC samples are derivatized by Ellman reaction, and then the TCEP content is measured by LC-MS/MS. This method can exclude the interference caused by the substances that may react with Ellman in complex ADC samples, which provides with no need for sample pretreatment, and with strong specificity.

Abstract: The present disclosure relates to a method for detecting DTPA content in ADC by LC-MS/MS. The samples are derivatized with Fe3+ under a weak alkaline derivatization condition, and a weak alkaline mobile phase is used, without need for addition of ion-pair reagents. This method has the characteristics of high efficiency, high stability and strong durability, and can well make up for the defects of few DTPA detection methods and complicated operations in the prior art.