Patents by Inventor Guiyang Shi
Guiyang Shi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117396Abstract: The present disclosure discloses a Bacillus licheniformis cell stably and repeatedly used for the conversion and synthesis of D-psicose, and belongs to the field of biotechnology. D-psicose is a functional sugar with high health value and has broad application prospects in food, medicine, health care and other fields. The B. licheniformis cell of the present disclosure efficiently expresses recombinant D-psicose-3-epimerase, and can convert high-concentration fructose as a substrate into D-psicose. After the whole-cell catalyst is reused 10 times, the conversion rate is not less than 30%. Compared with pure enzyme or single-use whole-cell conversion methods, this technology for the synthesis of D-psicose has significant advantages such as low catalyst cost and simple separation and purification.Type: ApplicationFiled: December 14, 2023Publication date: April 11, 2024Inventors: Youran LI, Zhiyong Wu, Guiyang Shi
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Patent number: 11946088Abstract: The disclosure discloses a method for processing oil crops with rhodotorula, and belongs to the technical field of fermentation. The method includes the step of inoculating the rhodotorula (such as Rhodotorula mucilaginosa, Sporidiobolus salmonicolor and Rhodotorula glutinis) that can produce carotenoid into a fermentation medium that contains oil-rich oil crops for solid state fermentation to obtain oil and oil crop meal rich in carotenoid. The carotenoid as a fermentative metabolite of the rhodotorula has bioactivities of resisting oxidation, preventing vascular sclerosis, enhancing immunity and preventing cancers. Contents of carotenoid in the oil and oil crop meal acquired by the method can be up to 9.071 ?g/g and 8.062 ?g/g correspondingly.Type: GrantFiled: June 18, 2021Date of Patent: April 2, 2024Assignee: JIANGNAN UNIVERSITYInventors: Yuanfa Liu, Zhongyang Ding, Yunzhi Jiang, Liting Zhao, Mengmeng Xu, Qi Zhang, Zhenghua Gu, Guiyang Shi
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Patent number: 11434467Abstract: Disclosed is an Aspergillus niger seed continuous culture method, comprising the steps of: (1) at a startup stage, Aspergillus niger spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture.Type: GrantFiled: December 24, 2018Date of Patent: September 6, 2022Assignees: Jiangsu Guoxin Union Energy Co., Ltd, JIANGNAN UNIVERSITYInventors: Guiyang Shi, Zhijie Hu, Youran Li, Xiaodong Jiang, Sai Jin, Fuxin Sun, Cheng Zhang, Dongjiao Zhou, Jiawei Lu, Maodong Miao, Zihao Fan
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Publication number: 20210322497Abstract: The disclosure discloses a method of fermenting soybeans or other oil crops by edible and medicinal fungi, and belongs to the technical field of fermentation. The method involves solid-state fermentation by inoculating edible and medicinal fungi such as i Ganoderma lucidum, Tremella aurantialba, Hericium erinaceus and Cordyceps sinensis that can produce bioactive substances such as active polysaccharides, flavonoids and triterpenoids in a fermentation substrate containing oily crops such as extruded soybeans, peanuts and rapeseeds, thereby obtaining oil and oil crop meal rich in the bioactive substances. In the oil obtained by the method, the content of flavonoids is as high as 0.302 mg/g, and the content of triterpenoids is as high as 65.663 mg/g. In the oil crop meal, the content of active polysaccharides is as high as 60.651 mg/g, the content of flavonoids is as high as 1.599 mg/g, and the content of triterpenoids is as high as 14.225 mg/g.Type: ApplicationFiled: June 19, 2021Publication date: October 21, 2021Inventors: Zhongyang DING, Yuanfa LIU, Yunzhi JIANG, Mengmeng XU, Liting ZHAO, Chen CAO, Qiong WANG, Zhenghua GU, Guiyang SHI
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Publication number: 20210310035Abstract: The disclosure discloses a method for processing oil crops with Rhodotorula, and belongs to the technical field of fermentation. The method includes the step of inoculating the Rhodotorula (such as Rhodotorula mucilaginosa, Sporidiobolus salmonicolor and Rhodotorula glutinis) that can produce carotenoid into a fermentation medium that contains oil-rich oil crops for solid state fermentation to obtain oil and oil crop meal rich in carotenoid. The carotenoid as a fermentative metabolite of the Rhodotorula has bioactivities of resisting oxidation, preventing vascular sclerosis, enhancing immunity and preventing cancers. Contents of carotenoid in the oil and oil crop meal acquired by the method can be up to 9.071 ?g/g and 8.062 ?g/g correspondingly.Type: ApplicationFiled: June 18, 2021Publication date: October 7, 2021Inventors: Yuanfa LIU, Zhongyang DING, Yunzhi JIANG, Liting ZHAO, Mengmeng XU, Qi ZHANG, Zhenghua GU, Guiyang SHI
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Publication number: 20200231929Abstract: Disclosed is an Aspergillus niger seed continuous culture method, comprising the steps of: (1) at a startup stage, Aspergillus niger spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture.Type: ApplicationFiled: December 24, 2018Publication date: July 23, 2020Applicants: Jiangsu Guoxin Union Energy Co.,Ltd, JIANGNAN UNIVERSITYInventors: Guiyang SHI, Zhijie HU, Youran LI, Xiaodong JIANG, Sai JIN, Fuxin SUN, Cheng ZHANG, Dongjiao ZHOU, Jiawei LU, Maodong MIAO, Zihao FAN
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Patent number: 10626380Abstract: The present invention provides a novel method for improving microbial laccase production, which relates to the field of microbial fermentation. The present invention is to add beta-carotene and other types of carotenoids, or microorganisms that produces carotenoids, or a mixture comprising carotenoids into a fermentation system during fermentation of Pleurotus ferulae and other higher fungi. The present invention can improve the laccase production 12 times more than before. With the advantage of a simple process and high yield, the present invention has a bright application prospect.Type: GrantFiled: April 4, 2019Date of Patent: April 21, 2020Assignee: Jiangnan UniversityInventors: Zhongyang Ding, Chaolin Guo, Liting Zhao, Bingxin Lu, Qiong Wang, Lin Peng, Jian Lu, Zhenghua Gu, Guiyang Shi
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Patent number: 10611992Abstract: The present invention discloses a method for rapidly characterizing content variations of triterpenoids in a liquid fermentation process of Antrodia camphorata, belonging to the field of microbial fermentation. According to the method of the present invention, in the liquid fermentation of Antrodia camphorata, an analysis method of rapidly judging the content variations of triterpenoids by rapid on-line or off-line detection and analysis of the content of a volatile aromatic substance ?-terpineol is utilized to implement automatic control of the fermentation process. Predictive analysis of triterpenoids in the fermentation process based on variations in on-line real-time parameters increases the controllability and production predictability of the fermentation process. This is of great significance for the development and utilization of Antrodia camphorata products having various bioactivities and application thereof in industrial production.Type: GrantFiled: November 1, 2018Date of Patent: April 7, 2020Assignee: JIANGNAN UNIVERSITYInventors: Zhongyang Ding, Mengmeng Xu, Zhenghong Xu, Zhijian Huang, Han Wang, Liting Zhao, Guiyang Shi, Gaoqiang Liu, Lianzhong Ai, Zhicai Zhang
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Publication number: 20190233801Abstract: The present invention provides a novel method for improving microbial laccase production, which relates to the field of microbial fermentation. The present invention is to add ?-carotene and other types of carotenoids, or microorganisms that producing carotenoids, or mixture comprising carotenoids into fermentation system during fermentation of Pleurotus ferulae and other higher fungi. The present invention can improve the laccase production for 12 times than before. With the advantages of simple process and high yield, the present invention has a bright application prospect.Type: ApplicationFiled: April 4, 2019Publication date: August 1, 2019Inventors: Zhongyang Ding, Chaolin Guo, Liting Zhao, Bingxin Lu, Qiong Wang, Lin Peng, Jian Lu, Zhenghua Gu, Guiyang Shi
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Patent number: 10266812Abstract: The present invention provides a novel method for improving microbial laccase production, which relates to the field of microbial fermentation. The present invention is to add ?-carotene and other types of carotenoids, or microorganisms that produce carotenoids, or mixtures comprising carotenoids into a fermentation system during fermentation of Pleurotus ferulae and other higher fungi. The present invention can improve the laccase production 12 times more than before, with the advantages of a simple process and high yield.Type: GrantFiled: September 19, 2016Date of Patent: April 23, 2019Assignee: Jiangnan UniversityInventors: Zhongyang Ding, Chaolin Guo, Liting Zhao, Bingxin Lu, Qiong Wang, Lin Peng, Jian Lu, Zhenghua Gu, Guiyang Shi
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Publication number: 20190071629Abstract: The present invention discloses a method for rapidly characterizing content variations of triterpenoids in a liquid fermentation process of Antrodia camphorata, belonging to the field of microbial fermentation. According to the method of the present invention, in the liquid fermentation of Antrodia camphorata, an analysis method of rapidly judging the content variations of triterpenoids by rapid on-line or off-line detection and analysis of the content of a volatile aromatic substance ?-terpineol is utilized to implement automatic control of the fermentation process. Predictive analysis of triterpenoids in the fermentation process based on variations in on-line real-time parameters increases the controllability and production predictability of the fermentation process. This is of great significance for the development and utilization of Antrodia camphorata products having various bioactivities and application thereof in industrial production.Type: ApplicationFiled: November 1, 2018Publication date: March 7, 2019Inventors: Zhongyang DING, Mengmeng XU, Zhenghong XU, Zhijian HUANG, Han WANG, Liting ZHAO, Guiyang SHI, Gaoqiang LIU, Lianzhong AI, Zhicai ZHANG
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Publication number: 20180002677Abstract: The present invention provides a novel method for improving microbial laccase production, which relates to the field of microbial fermentation. The present invention is to add ?-carotene and other types of carotenoids, or microorganisms that produce carotenoids, or mixtures comprising carotenoids into a fermentation system during fermentation of Pleurotus ferulae and other higher fungi. The present invention can improve the laccase production 12 times more than before, with the advantages of a simple process and high yield.Type: ApplicationFiled: September 19, 2016Publication date: January 4, 2018Inventors: Zhongyang Ding, Chaolin Guo, Liting Zhao, Bingxin Lu, Qiong Wang, Lin Peng, Jian Lu, Zhenghua Gu, Guiyang Shi