Patents by Inventor Gulilat Gebeyehu

Gulilat Gebeyehu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190264230
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Application
    Filed: November 21, 2018
    Publication date: August 29, 2019
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Publication number: 20190024167
    Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
    Type: Application
    Filed: June 28, 2018
    Publication date: January 24, 2019
    Inventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
  • Patent number: 10155780
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes. The present invention is in the field of molecular biology, and more particularly in the field of transfection, which utilizes new compounds and methods for the introduction of nucleic acids into eukaryotic cells.
    Type: Grant
    Filed: October 28, 2013
    Date of Patent: December 18, 2018
    Assignee: MOLECULAR TRANSFER, INC.
    Inventors: Gulilat Gebeyehu, Joel Jessee
  • Publication number: 20180340188
    Abstract: Disclosed herein are transfection complexes comprising at least one cell surface ligand; at least one helper lipid component; and a transfection enhancer. Also disclosed are pharmaceutical compositions comprising the disclosed transfection complexes, and a pharmaceutically acceptable carrier. Further, disclosed are methods of transfecting a cell, the method comprising the steps of: obtaining a transfection complex as disclosed; and contacting a cell with the transfection complex.
    Type: Application
    Filed: August 26, 2016
    Publication date: November 29, 2018
    Applicant: MOLECULAR TRANSFER, INC.
    Inventors: Gulilat GEBEYEHU, Joel JESSE
  • Patent number: 10138497
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: November 27, 2018
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Patent number: 10041117
    Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: August 7, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
  • Publication number: 20180002724
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Application
    Filed: September 18, 2017
    Publication date: January 4, 2018
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Patent number: 9765359
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Grant
    Filed: January 12, 2016
    Date of Patent: September 19, 2017
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Publication number: 20170253888
    Abstract: Disclosed herein are transfection complexes comprising at least one cell surface ligand; at least one helper lipid component; and a transfection enhancer. Also disclosed are pharmaceutical compositions comprising the disclosed transfection complexes, and a pharmaceutically acceptable carrier. Further, disclosed are methods of transfecting a cell, the method comprising the steps of: obtaining a transfection complex as disclosed; and contacting a cell with the transfection complex.
    Type: Application
    Filed: March 1, 2017
    Publication date: September 7, 2017
    Inventors: Gulilat GEBEYEHU, Joel JESSEE
  • Publication number: 20170029448
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes. The present invention is in the field of molecular biology, and more particularly in the field of transfection, which utilizes new compounds and methods for the introduction of nucleic acids into eukaryotic cells.
    Type: Application
    Filed: October 28, 2013
    Publication date: February 2, 2017
    Applicant: MOLECULAR TRANSFER, INC.
    Inventors: Gulilat GEBEYEHU, Joel JESSEE
  • Patent number: 9358300
    Abstract: Disclosed are compounds capable of facilitating transport of biologically active agents or substances into cells having the general structure: wherein Q is selected from the group consisting of N, O and S; L is any bivalent organic radical capable of linking each Q, such as C, CH, (CH2)l, or {(CH2)i—Y—(CH2)j}k, wherein Y is selected from the group consisting of CH2, an ether, a polyether, an amide, a polyamide, an ester, a sulfide, a urea, a thiourea, a guanidyl, a carbamoyl, a carbonate, a phosphate, a sulfate, a sulfoxide, an imine, a carbonyl, and a secondary amino group and wherein Y is optionally substituted by —X1-L?-X2—Z or —Z; R1 R6, independently of one another, are selected from the group consisting of H, —(CH2)p-D-Z, an alkyl, an alkenyl, an aryl, and an alkyl or alkyl ether optionally substituted by one or more of an alcohol, an aminoalcohol, an amine, an amide, an ether, a polyether, a polyamide, an ester, a mercaptan, an alkylthio, a urea, a thiourea, a guanidyl, or a carbamoyl group, and wh
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: June 7, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Yongliang Chu, Malek Masoud, Gulilat Gebeyehu
  • Publication number: 20160130607
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Application
    Filed: January 12, 2016
    Publication date: May 12, 2016
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Patent number: 9259475
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: February 16, 2016
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Publication number: 20160024138
    Abstract: The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.
    Type: Application
    Filed: June 24, 2015
    Publication date: January 28, 2016
    Inventors: Gulilat GEBEYEHU, Richard Pires
  • Patent number: 9085797
    Abstract: The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: July 21, 2015
    Assignee: Life Technologies Corporation
    Inventors: Gulilat Gebeyehu, Richard Pires
  • Publication number: 20150190522
    Abstract: Disclosed are compounds capable of facilitating transport of biologically active agents or substances into cells having the general structure: wherein Q is selected from the group consisting of N, O and S; L is any bivalent organic radical capable of linking each Q, such as C, CH, (CH2)l, or {(CH2)i—Y—(CH2)j}k, wherein Y is selected from the group consisting of CH2, an ether, a polyether, an amide, a polyamide, an ester, a sulfide, a urea, a thiourea, a guanidyl, a carbamoyl, a carbonate, a phosphate, a sulfate, a sulfoxide, an imine, a carbonyl, and a secondary amino group and wherein Y is optionally substituted by —X1-L?-X2—Z or —Z; R1 R6, independently of one another, are selected from the group consisting of H, —(CH2)p-D-Z, an alkyl, an alkenyl, an aryl, and an alkyl or alkyl ether optionally substituted by one or more of an alcohol, an aminoalcohol, an amine, an amide, an ether, a polyether, a polyamide, an ester, a mercaptan, an alkylthio, a urea, a thiourea, a guanidyl, or a carbamoyl group, and wh
    Type: Application
    Filed: July 21, 2014
    Publication date: July 9, 2015
    Inventors: Yongliang CHU, Malek MASOUD, Gulilat GEBEYEHU
  • Publication number: 20150037792
    Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
    Type: Application
    Filed: July 11, 2014
    Publication date: February 5, 2015
    Inventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
  • Publication number: 20140213637
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Application
    Filed: October 16, 2013
    Publication date: July 31, 2014
    Applicant: Molecular Transfer Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Patent number: 8785200
    Abstract: Disclosed are cationic lipid compounds and compositions of lipid aggregates for delivery of macromolecules and other compounds into cells. The compounds can be used alone or in combination with other compounds to prepare liposomes and other lipid aggregates suitable for transfection or delivery of compounds to target cells, either in vitro or in vivo. The compounds are preferably polycationic and preferably form highly stable complexes with various anionic macromolecules, particularly polyanions such as nucleic acids. These compounds have the property, when dispersed in water, of forming lipid aggregates which associate strongly, via their cationic portion, with polyanions. Also disclosed are intermediates for preparing the compound and compositions of the invention and methods of using the compounds to introduce other compounds into cells.
    Type: Grant
    Filed: March 14, 2012
    Date of Patent: July 22, 2014
    Assignee: Life Technologies Corporation
    Inventors: Yongliang Chu, Malek Masoud, Gulilat Gebeyehu
  • Patent number: 8759499
    Abstract: Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.
    Type: Grant
    Filed: March 28, 2011
    Date of Patent: June 24, 2014
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu