Patents by Inventor Gunter Bertholdt

Gunter Bertholdt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20160024462
    Abstract: The invention relates to a method for producing a cell culture (9) of human or animal cells, in particular mammalian cells, for the purpose of carrying out medical or pharmacological assays. According to the invention, the method comprises at least the following steps: inserting a substrate (3) of microbially produced cellulose into a vessel (1); introducing a nutrient solution (2) into the vessel (1); applying human or animal cells (4) to the substrate (3); conditioning the vessel contents to approximately body temperature; and waiting until the substrate (3) is completely covered by a cell lawn (5), i.e. confluence has been reached. Medical or pharmacological assays can then be carried out on the cell culture (9) thus produced.
    Type: Application
    Filed: March 25, 2014
    Publication date: January 28, 2016
    Applicant: XELLUTEC GMBH
    Inventor: Günter Bertholdt
  • Patent number: 8993324
    Abstract: In a process for the cultivation of living cells, in which the cells are cultivated on a support structure (14), the support structure (14) comprises cellulose. A process for the production of a support structure (14) of cellulose for the cultivation of living cells comprises the steps: preparation of a hollow mould; cultivation of cellulose-forming organisms in an interior space formed by the hollow mould, in order to allow the support structure (14) to grow in the interior space; demoulding of the hollow mould. In the step of demoulding the hollow mould, at least part (2, 3, 4) of the hollow mould is irreversibly deformed.
    Type: Grant
    Filed: February 12, 2008
    Date of Patent: March 31, 2015
    Assignee: Bioregeneration GmbH
    Inventor: Günter Bertholdt
  • Patent number: 8444700
    Abstract: An elongate hollow body (1) comprising crystalline cellulose has on an inside wall a plurality of protuberances (3, 4) which extend into a lumen of the hollow body (1). A process for the production of an elongate hollow body (1) comprising crystalline cellulose comprises the steps: preparation of a hollow mold (12); cultivation of cellulose-forming organisms in an interior space formed by the hollow mold (12), in order to allow the hollow body (1) to grow in the interior space; demolding of the hollow mold (12). In the step of demolding the hollow mold (12), at least part of the hollow mold (12) is irreversibly deformed.
    Type: Grant
    Filed: February 12, 2008
    Date of Patent: May 21, 2013
    Assignee: Bioregeneration GmbH
    Inventor: Günter Bertholdt
  • Patent number: 8251687
    Abstract: Process for the production of a long hollow cellulose body, where cellulose-forming organisms are cultivated in an interior space formed by a hollow mold, said space substantially having the shape of the long hollow body, in order to allow the long hollow body to grow in the interior space, wherein the direction of growth of the hollow body runs substantially from a first longitudinal side of the interior space to a second longitudinal side substantially opposite the first.
    Type: Grant
    Filed: February 19, 2007
    Date of Patent: August 28, 2012
    Assignee: Bioregeneration GmbH
    Inventor: Günter Bertholdt
  • Publication number: 20110319607
    Abstract: The invention relates to a method for producing a body (7) comprising cellulose with the help of cellulose-producing organisms, which are supplied with a nutrient solution. A particularly uniform structure and density of the cellulose bodies can be achieved when a vessel (1) having a membrane (4) is supplied, said membrane being permeable to the nutrient solution and is substantially impermeable to the organisms, wherein the organisms are cultivated on one side of the membrane (4), where a gas atmosphere prevails, and the nutrient solution is arranged on the other side of the membrane and passes through the membrane (4) in the direction of the organisms, to supply them.
    Type: Application
    Filed: November 9, 2009
    Publication date: December 29, 2011
    Applicant: BIOREGENERATION GMBH
    Inventors: Gunter Bertholdt, Dirk Weuster-Botz, Michael Hofinger, Katharina Seiffe
  • Publication number: 20100136691
    Abstract: In a process for the production of a structure comprising crystalline cellulose (10), cellulose (10) -forming organisms are at least partly cultured in a hollow mould (5). The hollow mould (5) is produced by means of a 3D printer (1), which builds up at least a part (2a, 2b) of the hollow mould (5) in layers from a modelling material.
    Type: Application
    Filed: April 10, 2008
    Publication date: June 3, 2010
    Applicant: bioregeneration GmbH
    Inventor: Günter Bertholdt
  • Publication number: 20100121431
    Abstract: An elongate hollow body (1) comprising crystalline cellulose has on an inside wall a plurality of protuberances (3, 4) which extend into a lumen of the hollow body (1). A process for the production of an elongate hollow body (1) comprising crystalline cellulose comprises the steps: preparation of a hollow mould (12); cultivation of cellulose-forming organisms in an interior space formed by the hollow mould (12), in order to allow the hollow body (1) to grow in the interior space; demoulding of the hollow mould (12). In the step of demoulding the hollow mould (12), at least part of the hollow mould (12) is irreversibly deformed.
    Type: Application
    Filed: February 12, 2008
    Publication date: May 13, 2010
    Applicant: Bioregeneration GmbH
    Inventor: Günter Bertholdt
  • Publication number: 20100041148
    Abstract: In a process for the cultivation of living cells, in which the cells are cultivated on a support structure (14), the support structure (14) comprises cellulose. A process for the production of a support structure (14) of cellulose for the cultivation of living cells comprises the steps: preparation of a hollow mould; cultivation of cellulose-forming organisms in an interior space formed by the hollow mould, in order to allow the support structure (14) to grow in the interior space; demoulding of the hollow mould. In the step of demoulding the hollow mould, at least part (2, 3, 4) of the hollow mould is irreversibly deformed.
    Type: Application
    Filed: February 12, 2008
    Publication date: February 18, 2010
    Applicant: bioregeneraton GmbH
    Inventor: Günter Bertholdt
  • Publication number: 20090011161
    Abstract: Process for the production of a long hollow cellulose body, where cellulose-forming organisms are cultivated in an interior space formed by a hollow mould, said space substantially having the shape of the long hollow body, in order to allow the long hollow body to grow in the interior space, wherein the direction of growth of the hollow body runs substantially from a first longitudinal side of the interior space to a second longitudinal side substantially opposite the first.
    Type: Application
    Filed: February 19, 2007
    Publication date: January 8, 2009
    Inventor: Gunter Bertholdt
  • Patent number: 5114854
    Abstract: For a microinjection of liquids or suspensions of substances into living cells, a stage and a cannula, which is movably mounted and extends at an oblique angle relative to the stage, are arranged to be movable relative to each other, the cells being contained in a culture vessel on the stage, the tip of the cannula constitutes a pointer and is moved in a plane z.sub.1, which is parallel to the stage and is disposed above the cells on the stage, the tip is moved to a position exactly over the location which is desired for an injection and thereafter, the cannula is moved away from the selected cell in the direction of the projection of the cannula on the plane and over a distance which depends on the angle between the cannula and the plane, the cannula is thus moved to a setback position, in which the axis of the cannula extends through the desired location, the cannula is then axially moved to puncture the cell and is subsequently reversed.
    Type: Grant
    Filed: February 21, 1989
    Date of Patent: May 19, 1992
    Assignee: Firma Eppendorf-Netheler-Hinz GmbH
    Inventor: Gunter Bertholdt