Abstract: Anti-selectin antibodies for reducing probability of incidence of polytraumatic events, such as organ failure.

Abstract: The present invention provides a process for the expression in appropriate host cells of recombinant DNA which is under the control of an inducible promotor. In order to obtain the soluble form of recombinant protein, which would usually be obtained in insoluble form, the induction of the promotor is limited to less than 10% of the maximum induction in comparison with a standard system and the transcription rate of the DNA coding for the protein is correspondingly limited.

Abstract: The invention provides an expression vector useful in expressing foreign genes in procaryotes. The vector consists of the promotor/operator region and initiation point of translation of the mg1 operon. This combination is referred to as a regulation sequence. In order to prepare this, desired portions of the mg1 operon are cut from the genome of a cell which can utilize galactose via appropriate restriction endonucleases. This is then inserted into a DNA vector.

Abstract: The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has1.) the nucleic acid sequence shown in FIG. (1),2.) a sequence corresponding to it within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.

Abstract: The invention concerns a recombinant DNA which contains(1) the sequence shown in SEQ ID NO: 1,(2) a sequence corresponding to this sequence within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent hybridizing conditionswhereby the DNA sequence codes for a protein with N-carbamoyl sarcosine amidohydrolase activity.Furthermore the invention concerns a recombinant vector with the DNA according to the present invention as well as a process for the isolation of a recombinant protein with N-carbamoyl sarcosine amidohydrolase activity and its use for the determination of creatinine.

Abstract: A distributing and smoothing tool has a grip (2) releasably connected to a trowel blade (1). A fastening rail (10) has a top hat shape, in cross section, with outwardly bent flanges (14,15) which rest on the upper face (16) of the trowel blade (1). The flanges (14, 15) are connected to the upper face (16) by rows of spot welds (17,18), one row along each flange. The grip (2) is supported by a grip base (5), which is appropriately formed in a U-shape in cross section, and positioned on the fastening rail (10). The end faces (26,27) of the limbs (6,7) of the grip base (5) are supported on the faces of the flanges (14,15) away from the trowel blade 1. An attachment (19) of the fastening rail (10), engages into a recess (20) of the grip base (5), and a knurled screw (23) engages into a threaded borehole (21) of the fastening rail (10) and secures to the grip base (5).

Abstract: For the preparation of cholesterol esterases with variable substrate specificity by cloning of a cholesterol esterase gene, the active center of which has the sequence -Gly-His-Ser-X-Gly-, wherein X signifies an amino acid, into a vector, transformation of a micro-organism with this vector and expression of the cholesterol esterase gene, one exchanges the amino acid X of the active center for another amino acid by mutagenesis.

Abstract: A frame for supporting a piece of equipment, particularly an air conditioning unit, onto the roof of a building is constructed using fastener means integral to the sheet metal so that the use of nuts, bolts or other fastening devices is eliminated. In particular, the fastening means comprise a slot cut into one piece of sheet metal and a dimple and tongue punched out of a second piece of sheet metal. The tongue is slipped into and over the slot until the dimple locks into the slot and thus holds the two pieces of sheet metal together.

Abstract: The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has(1) the nucleic acid sequence shown in FIG. (1),(2) a sequence corresponding to it within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.

Abstract: A frame for supporting a piece of equipment, particularly an air conditioning unit, onto the roof of a building is constructed using fastener means integral to the sheet metal so that the use of nuts, bolts or other fastening devices is eliminated. In particular, the fastening means comprises a slot cut into one piece of sheet metal and a dimple and tongue punched out of a second piece of sheet metal. The tongue is slipped into and over the slot until the dimple locks into the slot and thus holds the two pieces of sheet metal together.

Abstract: The present invention provides a process for the production of proteins or protein-containing gene products by transformation of eukaryotic host cells with a recombinant DNA molecule containing the gene for the desired protein, culturing the cells and isolating the gene product after expression, wherein, as host cells, there is used a yeast strain which is deficient in proteases A and B.

Abstract: The present invention provides a pyruvate oxidase which decarboxylates pyruvate to form inter alia hydrogen peroxide and is active without the addition of FAD, thiamine pyrophosphate and divalent metal ions. The amino acid sequence of the enzyme changes at least one proline in position 178 and alanine in position 458 to a different amino acid. The present invention also provides a process for the preparation of this pyruvate oxidase and methods of use thereof.

Abstract: A method of inactivating the biological activity of natural or recombinant DNA in a biomass, by adding a percarboxylic acid containing 1 to 3 carbon atoms, one of its salts, an alkali metal peroxide, or an alkali metal peroxomonosulfate and subsequently heating the mixture to 60.degree. to 100.degree. C.

Abstract: The present invention provides a pyruvate oxidase which decarboxylates pyruvate to form inter alia hydrogen peroxide and is active without the addition of FAD, thiamine pyrophosphate and divalent metal ions. The amino acid sequence of the enzyme changes at least one proline in position 178 and alanine in position 458 to a different amino acid. The present invention also provides a process for the preparation of this pyruvate oxidase and methods of use thereof.

Abstract: A stable mutant form of creatine amidohydrolase is disclosed. This enzyme is formed via expression of altered DNA, which may be in plasmid form. Also disclosed is a method for determining creatinine using the mutant form.

Abstract: A device for measuring elongation of a structure includes mounts formed at least partially of electrically insulating ceramic material, the mounts having at least two points to be attached to a structure for shifting the mounts relative to each other upon elongation of the structure, and electrically conducting parts disposed on the mounts having electrical properties being influenced by the shifting of the mounts, at least a portion of each of the electrically conducting parts being subjected to mechanical stresses, and at least the portions of the electrically conducting parts being formed of electrically conductive ceramic material.