Patents by Inventor GuoFan Hong

GuoFan Hong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
    Publication number: 20050089910
    Abstract: The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated florescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.
    Type: Application
    Filed: October 5, 2004
    Publication date: April 28, 2005
    Inventors: GuoFan Hong, Wei-hua Huang
  • DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
    Patent number: 6818431
    Abstract: The invention relates to genetic modification of DNA polymerase to reduce innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated fluorescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.
    Type: Grant
    Filed: February 24, 2000
    Date of Patent: November 16, 2004
    Assignee: Shanghai Mendel DNA Center Co. Ltd.
    Inventors: GuoFan Hong, Wei-hua Huang
  • Low-temperature cycle extension of DNA with high polymerization specificity
    Publication number: 20030087237
    Abstract: The invention relates to methods for extending a primer or a pair of primers in low-temperature cycle DNA amplification for cycle sequencing and PCR. In particular, the methods contemplate the combined use of moderately thermostable DNA polymerases in the presence of a low concentration of glycerol or ethylene glycol, or the mixtures thereof, as an agent to reduce the melting temperature of DNA (that is, the temperature at which the double-strands of DNA are denatured). Predistributed reaction mixtures of a high-fidelity and high processivity DNA polymerase stable at room temperature for several weeks in ready-to-use kits are also contemplated by the invention.
    Type: Application
    Filed: June 8, 2001
    Publication date: May 8, 2003
    Inventors: Guofan Hong, Yongjie Yang, Jia Zhu
  • DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
    Patent number: 6485909
    Abstract: The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated florescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.
    Type: Grant
    Filed: February 24, 2000
    Date of Patent: November 26, 2002
    Assignee: Shanghai Mendel DNA Center Co., Ltd.
    Inventors: GuoFan Hong, Wei-hua Huang
  • DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
    Patent number: 6165765
    Abstract: The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated florescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.
    Type: Grant
    Filed: September 21, 1998
    Date of Patent: December 26, 2000
    Assignee: Shanghai Institute of Biochemistry, Chinese Academy of Sciences
    Inventors: GuoFan Hong, Wei-hua Huang