Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.

Abstract: In some implementations, a radiofrequency down converter comprises an input port to receive a radiofrequency input signal, and the down converter includes a first bandpass filter configured to filter the input signal. The down converter includes a mixer stage coupled to the bandpass filter, the mixer stage being configured to generate a mixer output signal by processing the filtered input signal using a gain adjustment device, one or more amplifiers, and a mixer. The down converter includes a signal adjustment stage coupled to receive the mixer output signal, the signal adjustment stage comprising: a temperature compensation device configured to compensate for changes in signal gain due to changes in temperature; a second bandpass filter; a gain adjustment device; one or more amplifiers; and a low pass filter. The down converter comprises an output port coupled to output an adjusted mixer output signal from the signal adjustment stage.

Abstract: The present disclosure relates to unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. The disclosed compositions are useful for treating a subject with a disease or condition, such as a cardiovascular disease. In a further aspect, the cardiovascular disease can be a vascular stenosis or restenosis. Also described herein are methods of making and using the unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Abstract: Implementations of the subject matter described herein relate to mixed reality object rendering based on ambient light conditions. According to the embodiments of the subject matter described herein, while rendering an object a wearable computing device acquires light conditions of the real world, thereby increasing the reality of the rendered object. In particular, the wearable computing deice is configured to acquire an image of an environment where the wearable computing deice is located. The image is adjusted based on a cement parameter used when the image is captured. Subsequently, ambient light information is determined based on the adjusted image. In this way, the wearable computing deice can obtain more real and accurate emblem light information, so as to render to the user an object with enhanced reality. Accordingly, the user can have a better interaction experience.

Abstract: Aspects of the application provide methods of producing substrates having modified surfaces. In some aspects, methods of surface modification involve treating a surface of a substrate with an organic reagent in vapor phase to form an organic layer over the surface. In some aspects, methods of forming a stable surface coating on an oxidized surface are provided.

Abstract: The present invention provides cell assay systems and its use in cell based assays.

Abstract: A reciprocating cutting tool is provided, wherein the tool has a reciprocating saw mode and a jig saw mode. The tool includes a reciprocating mechanism; an actuating mechanism and a cutter-lifting mechanism. The reciprocating mechanism includes a reciprocating rod and the cutter-lifting mechanism includes an actuating member and a pushing member. The actuating member includes a first engaging portion and a second engaging portion when the reciprocating rod is driven to perform a reciprocating motion, the reciprocating rod drives the pushing member to deflect close to the first engaging portion or second engaging portion; when the first engaging portion or the second engaging portion is driven to move close to the pushing member, the first engaging portion or second engaging portion pushes the pushing member towards the side which the first engaging portion or the second engaging portion deflects to force the reciprocating rod to implement a cutter-lifting motion.

Abstract: In some implementations, a radiofrequency down converter comprises an input port to receive a radiofrequency input signal, and the down converter includes a first bandpass filter configured to filter the input signal. The down converter includes a mixer stage coupled to the bandpass filter, the mixer stage being configured to generate a mixer output signal by processing the filtered input signal using a gain adjustment device, one or more amplifiers, and a mixer. The down converter includes a signal adjustment stage coupled to receive the mixer output signal, the signal adjustment stage comprising: a temperature compensation device configured to compensate for changes in signal gain due to changes in temperature; a second bandpass filter; a gain adjustment device; one or more amplifiers; and a low pass filter. The down converter comprises an output port coupled to output an adjusted mixer output signal from the signal adjustment stage.

Abstract: Implementations of the subject matter described herein relate to mixed reality object rendering based on ambient light conditions. According to the embodiments of the subject matter described herein, while rendering an object a wearable computing device acquires light conditions of the real world, thereby increasing the reality of the rendered object. In particular, the wearable computing deice is configured to acquire an image of an environment where the wearable computing deice is located. The image is adjusted based on a cement parameter used w hen the image is captured. Subsequently, ambient light information is determined based on the adjusted image. In this way, the wearable computing deice can obtain more real and accurate emblem light information, so as to render to the user an object with enhanced reality. Accordingly, the user can have a better interaction experience.

Abstract: A cold atmospheric plasma (CAP)-mediated ICB therapy/delivery device are disclosed herein that employs a patch having microneedles that are used to deliver the CAP transdermally along with an immune checkpoint inhibitor for enhancing transdermal treatment efficacy. The hollow-structured microneedle patch can facilitate the transportation of CAP through the skin, causing tumor cell death. The release of cancer antigens then promotes the maturation of dendritic cells in the tumor-draining lymph nodes, subsequently initiating the T cell-mediated immune response. Anti-PDL1 antibody (aPDL1), an immune checkpoint inhibitor (or other immune checkpoint inhibitors), released from the microneedle patch (in some embodiments) further augments the anti-tumor immunity. The transdermal combinational CAP and ICB therapy inhibits tumor growth for both primary tumors as well as distant tumors, with prolonged survival in the tumor-bearing mice. Such results should translate to other species.

Abstract: Implementations of the subject matter described herein relate to mixed reality object rendering based on ambient light conditions. According to the embodiments of the subject matter described herein, while rendering an object, a wearable computing device acquires light conditions of the real world, thereby increasing the reality of the rendered object. In particular, the wearable computing device is configured to acquire an image of an environment where the wearable computing device is located. The image is adjusted based on a camera parameter used when the image is captured. Subsequently, ambient light information is determined based on the adjusted image. In this way, the wearable computing device can obtain more real and accurate ambient light information, so as to render to the user an object with enhanced reality. Accordingly, the user can have a better interaction experience.

Abstract: Methods of preparing surfaces of sample wells are provided. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form an antifouling overlay over a metal oxide surface of the sample well. In some aspects, methods of passivating and/or selectively functionalizing a sample well surface are provided.

Abstract: The present disclosure relates to unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. The disclosed compositions are useful for treating a subject with a disease or condition, such as a cardiovascular disease. In a further aspect, the cardiovascular disease can be a vascular stenosis or restenosis. Also described herein are methods of making and using the unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Abstract: A dust bin and a vacuum cleaner combination provided with the dust bin are provided. The dust bin includes a dust chamber, a dust inlet detachably connected to a dust suction apparatus, and a sealing structure for implementing the sealing between the dust suction apparatus and the dust bin. The dust suction apparatus has a dust outlet joined to the dust inlet. The vacuum cleaner combination includes a dust suction apparatus and a dust bin detachably connected to the dust suction apparatus. The dust suction apparatus has a housing and a dust cup assembly connected to the housing. The dust cup assembly includes a cup body and a filter apparatus disposed in the cup body. Compared with the prior art, in the present invention, a detachable multi-purpose dust bin with a simple structure is disposed, so that the dust collection chamber of the vacuum cleaner is flexibly increased.

Abstract: Aspects of the disclosure relate to methods and systems for regenerating a sensor chip surface, including techniques for reuse of a single sensor chip in multiple sampling cycles by regenerating a surface of the sensor chip between successive sampling cycles. A method is provided for reusing an integrated device to process a sample, the sample being divided into a plurality of aliquots, the method comprising: loading a first aliquot of the plurality of aliquots into at least some of a plurality of chambers of the integrated device; sampling analytes of the first aliquot while the analytes are present in the at least some of the plurality of chambers; removing the first aliquot from the at least some of the plurality of chambers of the integrated device; and loading a second aliquot of the plurality of aliquots into the at least some of the plurality of chambers of the integrated device.

Abstract: Methods and devices for ultrasensitive detection of target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices enable ultrasensitive determination of the concentration of target molecules.

Abstract: Provided herein are methods and integrated devices for improved sequencing of nucleic acid and peptide biomolecules. The present disclosure relates to improved mechanisms for protecting a luminescent label from photo-induced damage through the use of quenching moieties. Further provided herein are methods for improved immobilization of quenching moieties and other molecules of interest through functionalization with chemical moieties, such as click chemistry handles, capable of participating in cross-linking reactions. Quenching moieties may be immobilized to the surface of a sample well in a sequencing substrate or apparatus in a manner that minimizes or eliminates photobleaching of the labeled molecule. The disclosed methods provide for minimized photodamage, increased sensitivity, accuracy and length of reads during nucleic acid and polypeptide sequencing.

Abstract: Methods of preparing surfaces of sample wells are provided. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form an antifouling overlay over a metal oxide surface of the sample well. In some aspects, methods of passivating and/or selectively functionalizing a sample well surface are provided.

Abstract: Provided are a unimolecular nanoparticle, a composition thereof, and methods of use thereof, and includes 1) a dendritic polymer having a molecular weight of about 500-120,000 Da and terminating in hydroxyl, amino or carboxylic acid groups; 2) cationic polymers attached to at least a majority of the terminating groups of the dendritic polymer via a pH-sensitive linker, wherein each cationic polymer comprises a polymeric backbone attached to cationic functional groups and to weakly basic groups by disulfide bonds, wherein the molar ratio of cationic functional groups to weakly basic groups ranges from 1:1-5:1, and has a molecular weight from about 1,000-5,000 Da; and 3) poly(ethylene glycol) attached to a plurality of cationic polymers and having a terminal group selected from a targeting ligand, OH, O-alkyl, NH2 , biotin, or a dye, wherein the terminal group of at least one poly(ethylene glycol) is having a molecular weight of about 1,000-15,000 Da.